Czech Journal of Animal Science, 2007 (vol. 52), issue 5

Effect of pre-stimulation on milk flow pattern and distribution of milk constituents at a quarter level

V. Tančin, M. Uhrinčať, L. Mačuhová, R.M. Bruckmaier

Czech J. Anim. Sci., 2007, 52(5):117-121 | DOI: 10.17221/2234-CJAS  

The aim of this study was to investigate milk flow patterns and milk composition in relation to pre-milking udder stimulation. The milk of one quarter of each of the sixteen cows was removed separately and in the course of milking it was divided into six fractions (P – cisternal milk during milking without stimulation and the first 300 ml during milking with pre-stimulation, 0-25%, 25-50%, 50-75%, 75-100%, 75-100%, MS-machine stripping) and into five portions (25%, 50%, 75%, 100%, 100% + MS). Two milkings were performed during two consecutive evening milkings with or without manual stimulation. Pre-stimulation resulted in...

The effect of sex and slaughter weight on intramuscular fat content and its relationship to carcass traits of pigs

I. Bahelka, E. Hanusová, D. Peškovičová, P. Demo

Czech J. Anim. Sci., 2007, 52(5):122-129 | DOI: 10.17221/2233-CJAS  

Carcass quality and intramuscular fat content of castrates and gilts are compared and a possible dependence of intramuscular fat content on sex and slaughter weight is determined in the pig population of the Slovak Republic. A total of 129 pigs of three different genotypes were included in the experiment. After slaughter, the carcasses were weighed and backfat thickness was measured. On the next day, carcass dissection described by Walstra and Merkus (1995) was determined. Four prime cuts (shoulder, loin, ham and belly) were further dissected to meat, bones and fat with skin. Intramuscular fat content was analysed in a laboratory from the samples (100...

Lean and fat development in the whole body and hams of hybrid pigs studied by magnetic resonance tomography

V. Margeta, G. Kralik, G. Kušec, U. Baulain

Czech J. Anim. Sci., 2007, 52(5):130-137 | DOI: 10.17221/2230-CJAS  

The aim of this study was to determine the influence of MHS-genotype and feeding regime on the growth and development of muscle and fatty tissue in the whole body as well as in hams of hybrid pigs. The experiment was carried out on 72 barrows that were divided into 4 groups regarding the MHS-genotype (NN and Nn) and feeding regime (standard and intensive). Data necessary to determine the volume of examined tissues were obtained by means of magnetic resonance tomography. During the fattening period there were no statistically significant differences between pig groups with respect to total lean content of the body although the feeding regime effect...

An MspI PCR-RFLP detecting a single nucleotide polymorphism at alpha-lactalbumin gene in goat

Lan X.Y., C.Y. Pan, H. Chen, C.L. Zhang, A.L. Zhang, L. Zhang, J.Y. LI, C.Z. Lei

Czech J. Anim. Sci., 2007, 52(5):138-142 | DOI: 10.17221/2231-CJAS  

Alpha-lactalbumin (α-LA, LALBA) was strongly correlated with the nutritional value and the functional properties of whey and whey products. However, there are not many studies of goat LALBA gene and its polymorphisms in literary sources. In this paper, on the basis of PCR-SSCP and DNA sequencing, one M63868:g.1897T>C mutation in exon 3 at LALBA locus identified a Single Nucleotide Polymorphism (SNP): p.L100P of the LALBA protein, which could be detected by MspI endonuclease. Hence, we described a method based on MspI PCR-RFLP for SNP detection at the LALBA locus in 801 goats of China. Frequencies...

Possibilities of electrochemical techniques in metallothionein and lead detection in fish tissues

S. Křížková, O. Zítka, V. Adam, M. Beklová, A. Horna, Z. Svobodová, B. Sures, L. Trnková, L. Zeman, R. Kizek

Czech J. Anim. Sci., 2007, 52(5):143-148 | DOI: 10.17221/2232-CJAS  

In the present paper, we report on the use of adsorptive transfer stripping technique in connection with chronopotentiometric stripping analysis for metallothionein determination and of differential pulse anodic stripping voltammetry for lead detection in tissues of wild perch (Perca fluviatilis, n = 6) from the Svratka River in Brno, Czech Republic. Primarily, we determined the content of MT in tissues (muscles, gonads, liver and spleen) of perch. We measured the highest content of MT in spleen and liver (100-350 ng MT per gram of fresh weight). We assume that the content of MT determined in perch tissues is probably related with the...