Czech Journal of Animal Science, 2006 (vol. 51), issue 6

Methods of variance component estimation

D. Rasch, O. Mašata

Czech J. Anim. Sci., 2006, 51(6):227-235 | DOI: 10.17221/3933-CJAS  

Estimation of variance components is a method often used in population genetics and applied in animal breeding. Even experienced population geneticists nowadays feel lost if confronted with the huge set of different methods of variance component estimation. Especially because there exists no uniformly best method, a decision which method should be used is often difficult to take. This paper gives a complete overview of methods existing in the simplest case of a one-way lay-out and demonstrates some of them by a numerical example for which the true situation is known. Of course, the one-way lay-out is of limited practical interest but can best be used...

The relationship between conformations of dams and daughters in Czech Holsteins

J. Bouška, M. Vacek, M. Štípková, E. Němcová, P. Pytloun

Czech J. Anim. Sci., 2006, 51(6):236-240 | DOI: 10.17221/3934-CJAS  

The relationship between type traits in Holstein cows over generations was analysed. The analysed data set included a total of 12 157 dam-daughter pairs with linear scores of 14 type traits including a final score and general characteristics of type. Significant relationships existed between linear type traits in dams and their daughters. Pearson correlation coefficients ranged from r = 0.20 to r = 0.63 except the rump angle. A negative correlation (r = -0.02) was found for this trait. A similar tendency resulted from the analysis of the relationship between values of general characteristics and the final type score.

Milk protein genes CSN1S1, CSN2, CSN3, LGB and their relation to genetic values of milk production parameters in Czech Fleckvieh

J. Kučerová, A. Matějíček, O.M. Jandurová, P. Sørensen, E. Němcová, M. Štípková, T. Kott, J. Bouška, J. Frelich

Czech J. Anim. Sci., 2006, 51(6):241-247 | DOI: 10.17221/3935-CJAS  

Genotypes of milk protein genes, alphaS1-casein (CSN1S1), beta-casein (CSN2), kappa-casein (CSN3) and beta-lactoglobulin (LGB), were detected in 440 individuals of Czech Fleckvieh breed using a PCR-RFLP method. Breeding values of genotyped animals were obtained from the Official Database of Progeny Testing. A granddaughter design including 5 grandsires, 33 sons and 402 granddaughters was used for evaluation. Statistical analysis was carried out in SAS program using a restricted maximum likelihood method (REML). The aim of this study was to determine allele and genotype frequencies of observed genes and...

The pattern of development for gene expression of sterol regulatory element binding transcription factor 1 in pigs

J. Jiang, Z. XU, X. Han, F. Wang, L. Wang

Czech J. Anim. Sci., 2006, 51(6):248-252 | DOI: 10.17221/3936-CJAS  

Sterol regulatory element-binding transcription factor 1 (SREBF1) has been implicated as a key regulator of adipocyte differentiation and lipid metabolism. The pattern of SREBF1 gene expression in different growth stages and the relation with adipose deposition is studied. Fifteen female Duroc × Landrace × Yorkshire pigs in five groups, each group of three pigs at live weight 1, 30, 50, 70 and 90 kg, were used to study the developmental gene expression of SREBF1 in the subcutaneous adipose tissue by means of semi-quantitative RT-PCR. The results showed that porcine SREBF1 mRNA was present in a very low concentration...

The use of performance test parameters for selection of gilts before their placement into breeding

N. Kernerová, J. Václavovský, V. Matoušek, Z. Hanyková

Czech J. Anim. Sci., 2006, 51(6):253-261 | DOI: 10.17221/3937-CJAS  

The objectives of the paper were to evaluate carcass value in reared gilts on the basis of carcass analysis during the station test of purebred progeny of pigs and ultrasonically with Sonomark-100 or Piglog-105 instruments in the framework of valid methodology for performance testing, and to verify a pos-sibility of prediction of backfat thickness and/or average daily weight gain since birth and lean meat content of gilts as the elements of objectification for the evaluation of their body condition. In total 54 gilts of two breeds were evaluated: the dam breed Czech Large White and the sire breed Czech Large White - sire line. Four-parameter Richards...

The dynamics of biogenic amine formation in muscle tissue of carp (Cyprinus carpio)

P. Kordiovská, L. Vorlová, I. Borkovcová, R. Karpíšková, H. Buchtová, Z. Svobodová, M. Křížek, F. Vácha

Czech J. Anim. Sci., 2006, 51(6):262-270 | DOI: 10.17221/3938-CJAS  

The effect of various storage temperatures (3 ± 2°C; 24 ± 1°C and -18 ± 1°C) on the formation of biogenic amines in the muscle tissue of four hybrid lines of carp (Cyprinus carpio) with respect to microbial contamination was studied. Biogenic amines were determined by fluorescence detection HPLC based on pre-column dansylchloride derivatization. The studied microbiological parameters included total plate count, coliform bacteria count and psychrotrophic bacteria count. Concentrations of biogenic amines (putrescine, cadaverine, spermidine, spermine, tyramine, histamine and tryptamine) determined in the muscle tissue of...

Effect of different agronomical measures on yield and quality of autumn saved herbage during winter grazing - 2nd communication: Crude protein, energy and ergosterol concentration

W. Opitz Von Boberfeld, K. Banzhaf, F. Hrabe, J. Skladanka, S. Kozlowski, P. Golinski, L. Szeman, J. Tasi

Czech J. Anim. Sci., 2006, 51(6):271-277 | DOI: 10.17221/3939-CJAS  

In addition to the results published in the first communication (Opitz von Boberfeld et al., 2006) this paper presents crude protein, energy (ME) and ergosterol concentrations of autumn saved herbage at different sites in Central Germany, Poland, Hungary and the Czech Republic. Within these sites, the influence of the factors pre-utilisation and winter harvest date was tested over three consecutive years. Related to the different climatic conditions of the sites, crude protein concentrations of the growths pre-utilised in July varied from 149 g/kg in November to 134 g/kg of dry matter (DM) in January. The influence of climatic conditions was different...