Czech J. Anim. Sci., 2014, 59(1):26-34 | DOI: 10.17221/7191-CJAS

Estimation of the optimal ratio of standardized ileal digestible tryptophan to lysine for finishing barrows fed low protein diets supplemented with crystalline amino acidsOriginal Paper

C.Y. Xie1, G.J. Zhang1, F.R. Zhang1, S.H. Zhang1, X.F. Zeng1, P.A. Thacker2, S.Y. Qiao1
1 State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, P.R. China
2 Department of Animal and Poultry Science, University of Saskatchewan, Saskatoon, Canada

Optimum standardized ileal digestible (SID) tryptophan (Trp) to lysine (Lys) ratio was estimated for 67-96 kg barrows fed low protein diets supplemented with crystalline amino acids (AA). One hundred and fifty Yorkshire × Landrace barrows, with an average initial body weight (BW) of 67.3 ± 3.2 kg, were used in a 28-day feeding trial. All dietary treatments were based on corn, wheat bran, and soybean meal and were formulated to provide 10.5% crude protein and 12.6 MJ/kg metabolizable energy. The dietary SID Lys was set at 0.61% ensuring that Lys was marginally deficient for barrows of this weight range. Graded levels of crystalline l-Trp were added to the basal diet to produce diets providing SID Trp to Lys ratios of 0.131, 0.164, 0.197, 0.230, and 0.262. There were improvements in weight gain (linear and quadratic effect P < 0.01) and feed intake (linear effect P = 0.04) with increasing dietary SID Trp to Lys ratio. Increased SID Trp to Lys ratio resulted in a decrease in the serum urea nitrogen (SUN) content (linear and quadratic effect P < 0.01). The serum concentration of Trp increased with increasing dietary SID Trp to Lys ratio (linear effect P = 0.03, quadratic effect P = 0.08). Estimates of the optimum SID Trp to Lys ratios were 0.203, 0.197, and 0.214 for weight gain, feed conversion ratio, and SUN, respectively, using a broken-line model while a quadratic model produced optimum SID Trp to Lys ratios of 0.251, 0.224, and 0.249 for the same parameters. The results of this experiment indicate that the SID Trp to Lys ratio for finishing barrows is at least 0.203, which is higher than the SID ratio of Trp to Lys currently recommended by the National Research Council (NRC, 2012).

Keywords: tryptophan requirement; pig; performance; amino acid ratio

Published: January 31, 2014  Show citation

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Xie CY, Zhang GJ, Zhang FR, Zhang SH, Zeng XF, Thacker PA, Qiao SY. Estimation of the optimal ratio of standardized ileal digestible tryptophan to lysine for finishing barrows fed low protein diets supplemented with crystalline amino acids. Czech J. Anim. Sci. 2014;59(1):26-34. doi: 10.17221/7191-CJAS.
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    References

    1. AOAC (2003): Official Methods of Analysis. 17th Ed. Association of Official Analytical Chemists, Arilington, USA.
    2. ARC (1981): The Nutrient Requirements of Pigs. Commonwealth Agricultural Bureau, Farnham Royal, UK.
    3. Baker D.H. (1986): Problems and pitfalls in animal experiments designed to establish dietary requirements for essential nutrients. Journal of Nutrition, 116, 2339-2349. Go to original source... Go to PubMed...
    4. Boisen S. (2003): Ideal dietary amino acid profiles for pigs. In: D'Mello J.P.F. (ed.): Amino Acids in Animal Nutrition. CABI Publishing, Edinburgh, UK, 157-168. Go to original source...
    5. Boisen S., Hvelplund T., Weisbjerg M.R. (2000): Ideal amino acid profiles as a basis for feed protein evaluation. Livestock Production Science, 64, 239-251. Go to original source...
    6. BSAS (2003): Nutrient Requirement Standards for Pigs. British Society of Animal Science, Penicuik, UK.
    7. CAAS (2009): Tables of Feed Composition and Nutritive Value. 20th Ed. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
    8. Chung T.K., Baker D.H. (1992): Ideal amino acid pattern for 10-kilogram pigs. Journal of Animal Science, 70, 3102-3111. Go to original source... Go to PubMed...
    9. Cromwell G.L. (2004): Identifying the limiting amino acids in complex and cereal grain-based diets to minimize nitrogen excretion. In: Proc. of the Midwest Swine Nutrition Conference. Ohio University Press, Columbus, USA, 69-83.
    10. D'Mello J.P.F. (2003): An outline of pathways in amino acid metabolism. In: D'Mello J.P.F. (ed.): Amino Acids in Animal Nutrition. CABI Publishing, Edinburgh, UK, 71-86. Go to original source...
    11. Eder K., Nonn H., Kluge H., Peganova S.(2003): Tryptophan requirement of growing pigs at various body weights. Journal of Animal Physiology and Animal Nutrition, 87, 336-346. Go to original source... Go to PubMed...
    12. Fuller M.F., McWilliam R., Wang T.C., Giles L.R. (1989): The optimum dietary amino acid pattern for growing pigs. 2. Requirements for maintenance and for tissue protein accretion. British Journal of Nutrition, 62, 255-267. Go to original source... Go to PubMed...
    13. Guzik A.C., Shelton J.L., Southern L.L., Kerr B.J., Bidner T.D. (2005): The tryptophan requirement of growing and finishing barrows. Journal of Animal Science, 83, 1303-1311. Go to original source... Go to PubMed...
    14. Kendall D.C., Gaines A.M., Kerr B.J., Allee G.L. (2007): True ileal digestible tryptophan to lysine ratios in ninety- to one hundred twenty-five-kilogram barrows. Journal of Animal Science, 85, 3004-3012. Go to original source... Go to PubMed...
    15. Kerr B.J., McKeith F.K., Easter R.A. (1995): Effects on performance and carcass characteristics of nursery to finisher pigs fed reduced crude protein, amino acid-supplemented diets. Journal of Animal Science, 73, 433-440. Go to original source... Go to PubMed...
    16. Le Floc'h N., Jondreville C., Matte J.J., Seve B. (2006): Importance of sanitary environment for growth performance and plasma nutrient homeostasis during the post-weaning period in piglets. Archives of Animal Nutrition, 60, 23-34. Go to original source... Go to PubMed...
    17. Le Floc'h N., Gondret F., Matte J.J., Quesnel H. (2012): Towards amino acid recommendations for specific physiological and patho-physiological states in pigs. Proceedings of the Nutrition Society, 71, 425-432. Go to original source... Go to PubMed...
    18. Lewis A.J. (2001): Amino acids in swine nutrition. In: Lewis A.J., Southern L.L. (eds): Swine Nutrition. CRC Press, Boca Raton, USA, 145-164. Go to original source...
    19. Loughmiller J.A., Nelssen J.L., Goodband R.D., Tokach M.D., Titgemeyer E.C., Kim I.H. (1998): Influence of dietary total sulfur amino acids and methionine on growth performance and carcass characteristics of finishing gilts. Journal of Animal Science, 76, 2129-2137. Go to original source... Go to PubMed...
    20. NRC (2012): Nutrient Requirement of Swine. 11th Ed. National Academies Press, Washington, USA.
    21. NSNG (2010): National Swine Nutrition Guide Tables on Nutrient Recommendations, Ingredient Composition, and Use Rates. US Pork Center of Excellence, Ames, USA.
    22. Pedersen C., Boisen S. (2001): Studies on the response time for plasma urea nitrogen as a rapid measure for dietary protein quality in pigs. Animal Science, 51, 209-216. Go to original source...
    23. Pesti G.M., Vedenov D., Cason J.A., Billard L. (2009): A comparison of methods to estimate nutritional requirements from experimental data. British Poultry Science, 50, 16-32. Go to original source... Go to PubMed...
    24. Quant A.D. (2008): Standardized ileal digestible tryptophan to lysine ratios in growing pigs fed U.S. type and non U.S. type feedstuffs. MSc Thesis. University of Kentucky, Lexington, USA.
    25. Rademacher M., Sauer W.C., Jansman A.J.M. (2009): Standardized Ileal Digestibility of Amino Acids in Pigs. Evonik Degussa GmbH, Hanau-Wolfgang, Germany.
    26. Robbins K.R., Saxton A.M., Southern L.L. (2006): Estimation of nutrient requirements using broken-line regression analysis. Journal of Animal Science, 84, E155-E165. Go to original source... Go to PubMed...
    27. Stein H.H., Pedersen C., Wirt A.R., Bohlke R.A. (2005): Additivity of values for apparent and standardized ileal digestibility of amino acids in mixed diets fed to growing pigs. Journal of Animal Science, 83, 2387-2395. Go to original source... Go to PubMed...
    28. Stein H.H., Seve B., Fuller M.F., Moughan P.J., de Lange C.F. (2007a): Invited review: Amino acid bioavailability and digestibility in pig feed ingredients: terminology and application. Journal of Animal Science, 85, 172-180. Go to original source... Go to PubMed...
    29. Stein H.H., Fuller M.F., Moughan P.J., Seve B., Mosenthin R., Jansman A.J.M., Fernandez J.A., de Lange C.F.M. (2007b): Definition of apparent, true, and standardized ileal digestibility of amino acids in pigs. Livestock Science, 109, 282-285. Go to original source...
    30. Susenbeth A. (2006): Optimum tryptophan : lysine ratio in diets for growing pigs: analysis of literature data. Livestock Science, 101, 32-45. Go to original source...
    31. Tuitoek K., Young L.G., de Lange C.F., Kerr B.J. (1997): The effect of reducing excess dietary amino acids on growingfinishing pig performance: an evaluation of the ideal protein concept. Journal of Animal Science, 75, 1575-1583. Go to original source... Go to PubMed...
    32. Wang T.C., Fuller M.F. (1989): The optimum dietary aminoacid pattern for growing pigs. 1. Experiments by aminoacid deletion. British Journal of Nutrition, 62, 77-89. Go to original source... Go to PubMed...
    33. Wiltafsky M.K., Schmidtlein B., Roth F.X. (2009): Estimates of the optimum dietary ratio of standardized ileal digestible valine to lysine for eight to twenty-five kilograms of body weight pigs. Journal of Animal Science, 87, 2544-2553. Go to original source... Go to PubMed...
    34. Zhang G.J., Song Q.L., Xie C.Y., Chu L.C., Thacker P.A., Htoo J.K., Qiao S.Y. (2012): Estimation of the ideal standardized ileal digestible tryptophan to lysine ratio for growing pigs fed low crude protein diets supplemented with crystalline amino acids. Livestock Science, 149, 260-266. Go to original source...

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