Czech Journal of Animal Science, 2019 (vol. 64), issue 2

Expression of ESR1, PRLR, GHR, and IGF1R in mammary glands of Hu sheep with four teatsOriginal Paper

Lei Zhang, Fang Peng, Fangbai Yu, Lu Wan, Zhan-Qin Zhou

Czech J. Anim. Sci., 2019, 64(2):49-58 | DOI: 10.17221/3/2018-CJAS  

Supernumerary teats are commonly found in sheep. To investigate the expression levels of hormone receptors in supernumerary teats of Hu sheep, mammary tissue samples were collected from two groups of Hu sheep, four- and two-teat sheep, during adolescent (A), pregnancy (P), lactation (L), and non-pregnancy (N). Using hematoxylin and eosin staining, we found that mammary alveoli were denser in the four-teat sheep than in the two-teat sheep during P and L. Immunohistochemistry and Western blotting were used to detect the expression patterns and relative protein expression levels of the estrogen receptor 1 (ESR1), prolactin receptor (PRLR),...

Comparison of different semen extenders and cryoprotectant agents to enhance cryopreservation of rabbit spermatozoaOriginal Paper

Paula Domingo, Maite Olaciregui, Noelia González, Ignacio De Blas, Lydia Gil

Czech J. Anim. Sci., 2019, 64(2):59-66 | DOI: 10.17221/53/2018-CJAS  

The purpose of this research was to find a suitable protocol to enhance frozen rabbit sperm preservation analysing the role that seminal plasma (SP) plays and the effect of different cryoprotectant agents on sperm quality 0 and 2 h after thawing. Sperm samples were pooled and divided in eight fractions. Four of them were diluted with BotuCrio^® (extender A), INRA 96^® plus 6% glycerol (extender B), 6% N, N-dimethylformamide (extender C) and 6% N-methyl-2-pyrrolidone (extender D), respectively. The other four fractions were centrifuged and the supernatant was discarded in order to eliminate SP. Each sample was then resuspended...

Using nuclear microsatellite data to trace the gene flow and population structure in Czech horsesOriginal Paper

Lenka Putnová, Radek Štohl, Irena Vrtková

Czech J. Anim. Sci., 2019, 64(2):67-77 | DOI: 10.17221/2/2018-CJAS  

Based on a data set comprising 2879 animals and 17 nuclear microsatellite DNA markers, we propose the most comprehensive in-depth study mapping the genetic structure and specifying the assignment success rates in horse breeds at the Czech population scale. The STRUCTURE program was used to perform systematic Bayesian clustering via the Markov chain Monte Carlo estimation, enabling us to explain the population stratification and to identify genetic structure patterns within breeds worldwide. In total, 182 different alleles were found over all the populations and markers, with the mean number of 10.7 alleles per locus. The expected heterozygosity ranged...

Influence of interspecific hybridization on fitness-related traits in Siberian sturgeon and Russian sturgeonOriginal Paper

Sahana Shivaramu, Doi Thi Vuong, Miloš Havelka, Hana Šachlová, Ievgen Lebeda, Vojtěch Kašpar, Martin Flajšhans

Czech J. Anim. Sci., 2019, 64(2):78-88 | DOI: 10.17221/165/2018-CJAS  

Polyploidy in sturgeons makes them highly susceptible to interspecific hybridization, and these interspecific hybrids have been described in nature as well as in captivity. Nevertheless, the fitness-related traits between sturgeon hybrids and pure species have been poorly compared as yet. In the present study, we compared the reproductive parameters such as fertilization rate and hatching rate, growth traits and genetic polymorphism in the artificially produced hybrids of the Siberian sturgeon (Acipenser baerii) and Russian sturgeon (A. gueldenstaedtii) with their purebreds. Fertilization and hatching rates were found to...

mRNA Expression of CYP2E1, CYP2A19, CYP1A2, HSD3B, SULT1A1 and SULT2A1 genes in surgically castrated, immunologically castrated, entire male and female pigs and correlation with androstenone, skatole, indole and Improvac-specific antibody levelsOriginal Paper

Anna Kubešová, Kamil Šťastný, Martin Faldyna, Zbyšek Sládek, Iva Steinhauserová, Gabriela Bořilová, Aleš Knoll

Czech J. Anim. Sci., 2019, 64(2):89-97 | DOI: 10.17221/159/2018-CJAS  

This study aimed to obtain a comprehensive look at the influence of castration on mRNA expression of the genes CYP2E1, CYP1A2, CYP2A19, HSD3B, SULT2A1 and SULT1A1 and their correlation with boar taint compounds (androstenone, skatole and indole) and Improvac-specific antibodies in a Czech commercial hybrid (Large White × Landrace (sow) × Duroc (boar)). Pigs were divided into groups of entire male pigs (NC), pigs castrated surgically (SC), pigs immunologically castrated and slaughtered 8 weeks (IM8) or 15 weeks (IM15) after the second dose of Improvac, and gilts (GI). Hepatic mRNA expression, measured by quantitative...