Czech Journal of Animal Science, 2016 (vol. 61), issue 1

Kinetics of hydrogen peroxide generated from live and dead ram spermatozoa and the effects of catalase and oxidase substrates additionOriginal Paper

M. Alomar, M. Alzoabi, M. Zarkawi

Czech J. Anim. Sci., 2016, 61(1):1-7 | DOI: 10.17221/8662-CJAS  

The generation of hydrogen peroxide (H2O2) by ram spermatozoa (spz) was measured using a flurometric assay with 10-acetyl-3,7-dihydroxyphenoxazine agent as a probe for H2O2 detection. The kinetics of H2O2 production from both live and dead spz at 1 × 106, 3 × 106, and6 × 106 spz/well concentrations were assessed in the tyrode albumin lactate (TAL) medium every 15 min for 120 min. An increase in H2O2 production from both live and dead spz was noted with a significant difference (P < 0.05) between the 1 × 106...

Feasibility of rearing brook char fingerlings in an intensive recirculating hatchery as a complementary species to rainbow troutOriginal Paper

M. Buřič, J. Bláhovec, J. Kouřil

Czech J. Anim. Sci., 2016, 61(1):8-14 | DOI: 10.17221/8663-CJAS  

Aquaculture, as the fastest growing agriculture sector, is currently focused on exploring the development of effective intensive recirculating systems (RAS). The use of intensive RAS requires a stable supply of fingerlings throughout the year. Salmonids are a highly important aquaculture species, with rainbow trout Oncorhynchus mykiss often reared in freshwater RASs. The dominant position of rainbow trout has triggered the investigation of a wider diversification of species, including brook char Salvelinus fontinalis. Brook char has the potential to be reared in facilities similar to those used for rainbow trout, but it is not known...

Inbreeding depression in the Lizard canary breed estimated by pedigree analysisOriginal Paper

F. Cecchi, G. Giacalone, G. Paci

Czech J. Anim. Sci., 2016, 61(1):15-21 | DOI: 10.17221/8664-CJAS  

The influence of inbreeding depression on phenotypic traits of the Lizard canary from an Italian breeder was evaluated. The following traits were studied: birds' life span, number of surviving offspring, and morphological traits (type of plumage, spangles, feather quality, breast, cap, ground colour, wings and tail, eyelashes, covert feathers, beak, legs and feet, habits, and size). The effects of inbreeding on genetic diseases and on the health of the breed were also considered. The inbreeding coefficient of each bird was computed by genealogical data. The distribution of inbreeding in the whole population was analyzed and eleven different inbreeding...

Acute sulforaphane action exhibits hormonal and metabolic activities in the rat: in vivo and in vitro studiesOriginal Paper

M. Okulicz, I. Hertig

Czech J. Anim. Sci., 2016, 61(1):22-31 | DOI: 10.17221/8665-CJAS  

So far, only the chronic effect of sulforaphane (SF) on metabolism was examined. This study sheds more light on SF potential ability of regulating lipid, carbohydrate, and hormonal metabolism during its acute action in in vivo and in vitro conditions. In the in vivo trial, rats were given once intragastrically 10 or 20 mg/kg of SF and were decapitated 4 h after the single intragastric treatment. The serum and the liver were collected to assay lipid, carbohydrate, and hormonal parameters. Additionally, we evaluated the acute direct in vitro action of SF (1.5 h) on basal and insulin-stimulated lipogenesis and basal...

Effects of dietary starch types on rumen fermentation and blood profile in goatsOriginal Paper

S.P. Wang, W.J. Wang, Z.L. Tan

Czech J. Anim. Sci., 2016, 61(1):32-41 | DOI: 10.17221/8666-CJAS  

The objective of the present study was to investigate how feeding a diet containing a type of purified starch entirely produced from normal or high amylose maize grains would affect rumen fermentation and blood profile in goats. Twenty rumen-cannulated growing goats were assigned equally to one of two dietary treatments according to a randomized controlled trial design and fed two total mixed rations with similar ingredients and chemical composition except for the types of purified maize starch. One type was the high amylose starch (HAS), and the other was the normal amylose starch (NAS). Compared to goats fed the NAS diet, goats fed the HAS diet had...

Preservation effects of melatonin on the quality and fertility of native Fars rooster semen during liquid storageOriginal Paper

M. Meamar, A.Z. Shahneh, M.J. Zamiri, S. Zeinoaldini, H. Kohram, M.R. Hashemi, S. Asghari

Czech J. Anim. Sci., 2016, 61(1):42-48 | DOI: 10.17221/8667-CJAS  

Liquid or frozen storage of poultry semen negatively affects the sperm motility and has crucial role in reducing fertility. Phospholipids in chicken sperm membrane are composed of high proportions of polyunsaturated fatty acids which are susceptible to lipid peroxidation. One of possible ways to improve semen quality is supplementing the ration with antioxidant compounds such as melatonin. In this study, seventy-two roosters were randomly divided into three equal groups. The first group (control; Group C) was exposed to 14 h light and 10 h darkness. The second group (Group M) was exposed to the same lighting period, but in combination with melatonion...