Czech Journal of Animal Science, 2015 (vol. 60), issue 8
Effects of supplementing vitamin E on in vitro rumen gas production, volatile fatty acid production, dry matter disappearance rate, and utilizable crude proteinOriginal Paper
C. Wei, S.X. Lin, J.L. Wu, G.Y. Zhao, T.T. Zhang, W.S. Zheng
Czech J. Anim. Sci., 2015, 60(8):335-341 | DOI: 10.17221/8402-CJAS 
Two in vitro trials were carried out to study the effects of supplementing vitamin E (VE) on rumen fermentation. In Trial I, four levels of VE product (purity 50%), i.e. 0, 15, 30, and 60 mg/kg dry matter (DM) of feed (equivalent to 0, 7.5, 15, 30 IU VE/kg DM) were supplemented to a typical feed mixture, respectively, as experimental treatments. The gas test technique of Menke et al. (1979) was used to measure gas and volatile fatty acid (VFA) production. In Trial II, the in vitro incubation technique of Zhao and Lebzien (2000) was used to determine DM disappearance rate and utilizable crude protein...
The role of cyclooxygenase inhibitors in lipopolysaccharide-induced hypophagia in chickenOriginal Paper
M. Zendehdel, A. Baghbanzadeh, B. Yeganeh, S. Hassanpour
Czech J. Anim. Sci., 2015, 60(8):342-350 | DOI: 10.17221/8403-CJAS
Previous studies showed that cyclooxygenase 1 (COX) enzyme has an important role in lipopolysaccharide (LPS)-induced hypophagia in mammals but the effect of COX on LPS-induced hypophagia has not been studied in avian species. The current study was designed to investigate the effects of Indomethacin, a non-selective cyclooxygenase inhibitor, Aspirin (irreversible cyclooxygenase inhibitor), Piroxicam (a selective COX-1 inhibitor), and Celecoxib (a selective COX-2 inhibitor) on LPS-induced hypophagia in 3-h food-deprived (FD3) cockerels. One hundred and sixty ROSS 308 chickens were randomly divided into 5 experiments and 4 treatment groups...
Effects of bovine colostrum on performance, survival, and immunoglobulin status of suckling piglets during the first days of lifeOriginal Paper
M. Viehmann, C. Unterweger, M. Ganter, B.U. Metzler-Zebeli, M. Ritzmann, I. Hennig-Pauka
Czech J. Anim. Sci., 2015, 60(8):351-358 | DOI: 10.17221/8404-CJAS
Supplementation of bovine colostrum (BC) has shown to improve growth performance, intestinal development, and immune response in early-weaned pigs. Little is known about whether BC may have similar effects in neonatal piglets. In the present study, the effect of BC supplementation on mortality, growth performance, and blood parameters (plasma proteins and white blood count) of suckling piglets in the first 10 days of life was investigated under practical conditions with special emphasis on low birth weight piglets. In total, 258 newborn piglets from 30 multiparous sows in a commercial breeding unit were randomly assigned to two different treatment...
Effects of chitosan on nitric oxide production and inducible nitric oxide synthase activity and mRNA expression in weaned pigletsOriginal Paper
J. Li, B. Shi, S. Yan, L. Jin, Y. Guo, T. Li
Czech J. Anim. Sci., 2015, 60(8):359-366 | DOI: 10.17221/8405-CJAS
The effects of chitosan on nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) activity and gene expression in vivo or vitro were investigated in weaned piglets. In vivo, 180 weaned piglets were assigned to five dietary treatments with six replicates. The piglets were fed on a basal diet supplemented with 0 (control), 100, 500, 1000, and 2000 mg chitosan/kg feed, respectively. In vitro, the peripheral blood mononuclear cells (PBMCs) from a weaned piglet were cultured respectively with 0 (control), 40, 80, 160, and 320 µg chitosan/ml medium. Results showed that serum NO concentrations on...
Age and sex-related changes in superoxide dismutase activity in bovine tissuesOriginal Paper
M. Giergiel, M. Kankofer
Czech J. Anim. Sci., 2015, 60(8):367-374 | DOI: 10.17221/8406-CJAS
The influence of age, gender, and type of tissue on superoxide dismutase (SOD) activity in bovine organs and tissues was investigated. The investigated material consisted of fragments of tissues and organs (liver, heart, lung, kidney, skeletal muscles, and diaphragm) from healthy cows (n = 15), bulls (n = 15), and female calves (n = 12) collected immediately after slaughter at the slaughterhouse. The total SOD activity was measured in tissue and organ homogenates by spectrophotometric method. PAGE electrophoresis and Western blotting technique with specific anti-SOD antibodies as well as zymography confirmed the presence...
Apparent ileal digestibility of protein and amino acids in protein feedstuffs and trypsin activity in the small intestine in broiler chickensOriginal Paper
M. Foltyn, M. Lichovníková, V. Rada, A. Musilová
Czech J. Anim. Sci., 2015, 60(8):375-382 | DOI: 10.17221/8407-CJAS
Coefficients of ileal apparent digestibility (CIAD) for crude protein (CP) and amino acids in five protein feedstuffs: corn distillers dried grains with solubles (DDGS), raw full-fat soybean (RFFSB), extruded full fat soybean (EFFSB), soybean meal (SBM), and rapeseed meal (RSM) were determined using 5-week-old male broiler chickens. Trypsin activity in digesta in the ileum and jejunum were also measured. CIAD of CP was the highest in SBM (0.73). The lowest (P < 0.05) CIAD of CP was determined for RFFSB (0.45). There was no significant difference in CIAD of CP between RSM (0.61) and DDGS (0.62). The CIAD values for all essential amino acids...