Czech Journal of Animal Science, 2015 (vol. 60), issue 6
Male pronucleus formation after ICSI: effect of oocyte cysteine or sperm Triton X-100 treatmentsOriginal Paper
E. Garcia-Mengual, M.A. Silvestre, I. Salvador, A. Cebrian-Serrano, E. Garcia-Rosello
Czech J. Anim. Sci., 2015, 60(6):241-249 | DOI: 10.17221/8237-CJAS 
In pigs, intracytoplasmic sperm injection (ICSI) efficiency is still poor. The inadequate decondensation of the sperm chromatin, its transformation into the male pronucleus (MPN) together with the subsequent inability to activate the oocyte, seem to be the main causes of the low ICSI efficiency. In order to improve the MPN formation we took two different approaches. On the one hand, the in vitro culture (IVC) medium post-ICSI was supplemented with 1.71mM cysteine (CYS). Alternatively, the sperm membrane was digested with Triton X-100 (TX) before ICSI, to improve the exposure of the sperm chromatin to the oocyte cytoplasm. After 6 h post-ICSI,...
Oxidative stress and motility in tench Tinca tinca spermatozoaOriginal Paper
V. Dzyuba, J. Cosson, B. Dzyuba, M. Rodina
Czech J. Anim. Sci., 2015, 60(6):250-262 | DOI: 10.17221/8238-CJAS
The attachment of the urinary bladder to the seminal duct near the anal aperture in tench constitutes a potential risk for urine contamination of sperm during collection, leading to spontaneous activation of sperm motility by urine hypotonicity. It was hypothesized that sperm hypotonic exposure can provoke oxidative stress which could be involved in sperm quality degradation. Our study aimed to describe spermatozoa motility parameters and levels of oxidative stress in activating media (AM) of differing osmolality. Tench sperm samples were collected from 6 males into Kurokura 180 immobilizing medium (IM) (180mM NaCl, 2.68mM KCl, 1.36mM CaCl2...
Association of heat shock protein 70 with motility of frozen-thawed sperm in bullsOriginal Paper
X.-G. Zhang, J.-Y. Hong, G.-J. Yan, Y.-F. Wang, Q.-W. Li, J.-H. Hu
Czech J. Anim. Sci., 2015, 60(6):256-262 | DOI: 10.17221/8239-CJAS
Heat shock protein 70 (HSP70) is considered as a gene which affects semen quality traits. The present study attempted to investigate the relationship between the HSP70 expression level and motility of bull sperm during the process of freezing-thawing. Semen samples were collected from 5 QinChuan bulls by artificial vagina. Sperm motility and plasma membrane integrity of the semen samples at three stages (fresh, after equilibration, and frozen-thawed) were evaluated. The HSP70 expression level at the three stages was detected using real-time PCR. The results indicated that HSP70 expression level, membrane...
Contribution of domestic and Interbull records to reliabilities of single-step genomic breeding values in dairy cattleOriginal Paper
J. Bauer, J. Přibyl, L. Vostrý
Czech J. Anim. Sci., 2015, 60(6):263-267 | DOI: 10.17221/8240-CJAS
The method of approximating reliabilities of genomic breeding values in the single-step genomic BLUP evaluation procedure of Misztal et al. (2013) was used to evaluate the increase in reliability of breeding values for milk production in dairy cattle brought about by the inclusion of genomic data. Three strategies for approximation of reliabilities were compared: using only domestic records from performance testing of cows in the Czech Holstein dairy cattle population, using the same records in combination with Interbull breeding values of sires expressed as deregressed proofs, and using only the Interbull breeding values of sires expressed as deregressed...
Time-dependent changes in milk fatty acid composition of ewes fed a winter ration supplemented with linseed or sunflower oilsOriginal Paper
L. Soják, J. Blaško, R. Kubinec, R. Górová, A. Hengerics Szabó, P. Májek, M. Margetín
Czech J. Anim. Sci., 2015, 60(6):268-280 | DOI: 10.17221/8241-CJAS
The effects of adding sunflower and linseed oils to a standard winter ration with a lower concentrate content on the milk fatty acid composition in lactating ewes were investigated. Eighteen dairy ewes randomly chosen from the ewe flock were divided into three groups: the first group was fed a winter ration, the second one a winter ration supplemented with sunflower oil, and the third a winter ration supplemented with linseed oil for a period of 12 days. In the treatment groups, the concentrate was partially replaced by 3.0 g/100 g sunflower or linseed oils. Milk samples were taken daily from morning milking for the analysis of fatty acid composition...
Estimation of genetic parameters for reproductive traits in Mehraban sheepOriginal Paper
R. Yavarifard, N. Ghavi Hossein-Zadeh, A.A. Shadparvar
Czech J. Anim. Sci., 2015, 60(6):281-288 | DOI: 10.17221/8242-CJAS
Genetic parameters for basic and composite reproductive traits in Mehraban sheep were estimated. Data included 10 257 records on reproductive performances of 5813 lambs from 69 sires and 603 dams which were collected from 1994 to 2011 in the Mehraban breeding station in Hamedan province, western Iran. Studied traits were litter size at birth (LSB), litter size at weaning (LSW), litter mean weight per lamb born (LMWLB), litter mean weight per lamb weaned (LMWLW), total litter weight at birth (TLWB), and total litter weight at weaning (TLWW). Test of significance to include fixed effects in the statistical model was performed using the GLM procedure...