Czech Journal of Animal Science, 2013 (vol. 58), issue 5

Retention of carotenoids in egg yolks of laying hens supplemented with heterotrophic Chlorella Original Paper

V. Kotrbáček, M. Skřivan, J. Kopecký, O. Pěnkava, P. Hudečková, I. Uhríková, J. Doubek

Czech J. Anim. Sci., 2013, 58(5):193-200 | DOI: 10.17221/6747-CJAS  

The present study investigated the effect of 1 and 2% feed supplementation with biomass of Chlorella grown through heterotrophic fermentation on the concentration of total and individual carotenoids in egg yolks. A total of twenty-four Hisex Brown laying hens aged 56 weeks were included in the experiment. The layers were kept individually in cages. They were divided into three groups of eight birds and fed a diet typical for laying hens. Control birds (C) received only a basal diet, while experimental diets (P1 and P2) were supplemented with 1 and 2% (i.e. 10 and 20 g/kg) dry disintegrated Chlorella biomass. Egg yolk deposition of...

Effects of a species-specific probiotic formulation on multiresistant Escherichia coli isolates from the gut of veal calvesOriginal Paper

B. Ripamonti, E. Tirloni, S. Stella, C. Bersani, A. Agazzi, S. Maroccolo, G. Savoini

Czech J. Anim. Sci., 2013, 58(5):201-207 | DOI: 10.17221/6748-CJAS  

In this study, 254 Escherichia coli isolates from faecal samples of veal calves were evaluated for antimicrobial susceptibility using the disk diffusion method. During the experimental period, six mass antibiotic treatments were administered to the animals (about one treatment per month). The active principles used were oxytetracycline, colistin, tylosin, doxycycline, chlortetracycline, and sulphonamides. An extremely high resistance prevalence (> 70%) towards penicillin, sulphonamide, tetracycline, ampicillin, and spyramicin was detected. Sixty E. coli isolates could be defined as multiresistant, showing resistance to at least...

Activity time budget patterns of sheep and goats co-grazing on semi-natural species-rich dry grasslandOriginal Paper

P. Pokorná, P. Hejcmanová, M. Hejcman, V. Pavlů

Czech J. Anim. Sci., 2013, 58(5):208-216 | DOI: 10.17221/6749-CJAS  

Activity time budget patterns and grazing response to sward and environmental conditions were investigated for paddocks of sheep and goats co-grazing for conservation purposes on a semi-natural species-rich dry grassland community endangered by shrub and tall perennial plant encroachment in a protected nature reserve in South Moravia (Czech Republic). Grazing was conducted by a rotational stocking system for 14 weeks in late grazing season in 2008 with 60 dry ewes and 20 goats. Twelve ewes and four goats were observed; grazing, ruminating, idling and other activities (salt licking, social interactions, walking), along with topographical position in...

Transcript analysis of Heat shock protein 72 and protein 53 of 4-cell mouse embryos following Cryotop vitrificationOriginal Paper

A. Habibi, N. Farrokhi, F. Morreira da Silva, A. Hosseini, B.F. Bettencourt, F. Amidi

Czech J. Anim. Sci., 2013, 58(5):217-226 | DOI: 10.17221/6750-CJAS  

The effects of two different concentrations of cryoprotectants on survival and developmental capacity of four-cell mouse embryos were compared by Cryotop vitrification to demonstrate that lower concentrations provide the same results as higher previously reported concentrations with lesser negative molecular impact on embryo cells. For this latest, embryos were compared via transcript analyses of Heat shock protein 72 (Hsp72) and protein 53 (p53). Four-cell embryos were obtained from superovulated female mice and randomly assignedto one of three following groups: (i) control (non-vitrified), (ii)...

Kaempferitrin improves meat quality of broiler chickensOriginal Paper

H.-B. Xiao, J. Fang, Z.-L. Sun

Czech J. Anim. Sci., 2013, 58(5):227-231 | DOI: 10.17221/6751-CJAS  

Angiopoietin-like protein 3 (Angptl3) may promote adipose formation. The objective of this study was to investigate the effect of kaempferitrin, a 3,7-diglycosyl flavone, on meat quality in broiler chickens and the mechanisms involved. One thousand two hundred broiler chickens were offered commercial diet that was supplemented with 0.0 (control), 0.1, 0.3, or 0.9% kaempferitrin, respectively. After 42 days, kaempferitrin (0.3 or 0.9%) treatment significantly increased the lightness of meat colour. Kaempferitrin (0.3 or 0.9%) supplementation decreased breast muscle drip loss, breast muscle crude fat, breast muscle malondialdehyde level, and hepatic...

Intestinal histology of newborn goat kids fed lyophilized bovine colostrumOriginal Paper

W.M. Nordi, D.B. Moretti, A.L. Lima, P. Pauletti, I. Susin, R. Machado-Neto

Czech J. Anim. Sci., 2013, 58(5):232-241 | DOI: 10.17221/6752-CJAS  

Enteric histology of newborn goat kids fed lyophilized bovine colostrum (LBC) was studied. At 0, 7, and 14 h of life 15 male newborns received 5% of body weight of lyophilized bovine colostrum and 14 male newborns goat colostrum (GC), both with 55 mg/ml of IgG. Samples of duodenum, jejunum, and ileum were collected at 18, 36, and 96 h of life for analyses of villus height, crypt depth, muscle layer thickness, partial volume of the absorptive mucosa (Vv), density of the absorptive mucosa (Sv), and quantification of goblet cells. Three animals were sampled without colostrum intake (0 h). The histomorphometry was not different between GC and LBC in all...