Czech Journal of Animal Science, 2013 (vol. 58), issue 11

The association of CAPN1, CAST, SCD, and FASN polymorphisms with beef quality traits in commercial crossbred cattle in the Czech RepublicOriginal Paper

K. Kaplanová, A. Dufek, E. Dračková, J. Simeonovová, J. Šubrt, I. Vrtková, J. Dvořák

Czech J. Anim. Sci., 2013, 58(11):489-496 | DOI: 10.17221/7044-CJAS  

Genetic variability of four polymorphisms (CAPN1, CAST, SCD, and FASN) was evaluated and the relationships between them and the beef quality traits (tenderness and fatty acid composition) in Czech crossbred cattle population were assessed. Totally 331 animals were genotyped using multiplex PCR-RFLP. For the CAST NM174003.2:c.155C>T the CC genotype was associated with higher Warner-Bratzler shear force (CC > CT, P < 0.001; CC > TT, P < 0.05) and our results (CC > TT > CT) suggest a possible effect of overdominance. The CAPN1 AF252504.2:c.947G>C...

Effects of supplementary vegetable and animal fats on the slaughter values of fatteners, meat quality, and fatty acid profile in pigsOriginal Paper

D. Lisiak, E. Grześkowiak, K. Borzuta, S. Raj, P. Janiszewski, G. Skiba

Czech J. Anim. Sci., 2013, 58(11):497-511 | DOI: 10.17221/7045-CJAS  

The study was conducted on 32 hybrids of (Polish Large White × Danish Landrace) × Duroc breeds fed similar energy content feed, however differing in fat diet supplements. The animals were divided into four groups in which the following fat supplements were added to the feed: A - rapeseed and linseed oils, B - rapeseed and fish oils + swine fat, C - linseed and fish oils, D - rapeseed and linseed oils + lard. The carcass slaughter value was estimated and physicochemical and sensory traits of longissimus lumborum (LL) and triceps brachii (TB) muscles were determined. The content of fatty acids in the subcutaneous fat of the loin as...

The luteal blood flow, area and pixel intensity of corpus luteum, levels of progesterone in pregnant and nonpregnant mares in the period of 16 days after ovulationOriginal Paper

J. Šichtař, R. Rajmon, K. Hošková, D. Řehák, L. Vostrý, H. Härtlová

Czech J. Anim. Sci., 2013, 58(11):512-519 | DOI: 10.17221/7046-CJAS  

The objective of the present study was to examine if luteal blood flow (LBF) monitoring could be used as an additional prognostic tool for early pregnancy diagnosis, and we particularly focused on the differences in LBF between pregnant and nonpregnant mares. Furthermore, other possible developmental differences of corpus luteum (CL) between pregnant and nonpregnant mares were evaluated. The CL (n = 119) of 27 mares were monitored once daily in B- and Power-Doppler Mode on days 1, 2, 9, 12, and 16 after ovulation (day 0 = ovulation). The data were evaluated using the MIXED Linear Model with repeated measures, and parameters were estimated...

Fertility enhancing effects of methanolic leaf extract of Dracaena arborea in albino rats (Rattus norvegicus)Original Paper

S.O. Ekere, C.N. Okoye, A.F. Udoumoh

Czech J. Anim. Sci., 2013, 58(11):520-524 | DOI: 10.17221/7047-CJAS  

The effects of methanolic extract of Dracaena arborea on mean testicular weight, mean cauda epididymal sperm reserve, and testicular morphology were evaluated. A total of sixty mature male Sprague Dawley rats were divided into three equal groups. The first group (A) received distilled water while the other two groups (B and C) received orally the methanolic leaf extracts of Dracaena arborea in two doses (100 and 500 mg/kg, respectively) daily for 84 days. Following oral administration of the extract, mean testicular weight, mean cauda epididymal sperm reserve, and testicular morphology were determined on days 28, 42, 56, 70, and 84....

Novel SNPs of the porcine TRIP12 are associated with water holding capacity of meatOriginal Paper

H.T.P. Loan, E. Murani, S. Maak, S. Ponsuksili, K. Wimmers

Czech J. Anim. Sci., 2013, 58(11):525-533 | DOI: 10.17221/7048-CJAS  

Degradation of proteins during maturation of meat, mediated by the calpain/calpastatin system and the ubiquitination system, largely affects the tenderness and the water holding capacity (WHC) of meat. The thyroid hormone receptor interacting protein 12 (TRIP12) is known as a HECT domain-containing E3 ubiquitin-protein ligase that recognizes protein substrates for ubiquitination. This study aims to identify polymorphisms of the TRIP12 gene and to evaluate the relationship between genotype, transcript abundance, and meat quality traits in pigs. Two synonymous SNPs (XM_003484315.1:c.2211T>C, c.4957A>C) were identified that segregated...