Czech Journal of Animal Science, 2011 (vol. 56), issue 8
Association analysis of seven candidate genes with performance traits in Czech Large White pigs
F. Weisz, T. Urban, P. Chalupová, A. Knoll
Czech J. Anim. Sci., 2011, 56(8):337-344 | DOI: 10.17221/169/2010-CJAS 
In this study association analyses were performed between genes tagged SNP (IGF2, NAMPT, DGAT1, MYF4, MC3R, MC4R and MYOD1), performance traits (backfat thickness, lean meat content, average daily gain from birth to the end of the test, average daily gain in test) and estimated breeding values (EBVs) in a population of Czech Large White sows (n = 101). Genotyping of all SNPs was performed by SNapShot with the exception of SNP within NAMPT gene for which HpaII PCR-RFLP assay was used. The following significant associations between genes tagged SNPs and traits or EBVs were found out: DGAT1...
The effect of depuration on heavy metals, petroleum hydrocarbons, and microbial contamination levels in Paphia undulata (Bivalvia: Veneridae)
M.M. El-Gamal
Czech J. Anim. Sci., 2011, 56(8):345-354 | DOI: 10.17221/2395-CJAS
The depuration of 8 heavy metals (Zn, Pb, Ni, Mn, Cu, Cr, Co and Cd), total petroleum hydrocarbons and pathogenic bacteria of Paphia undulata was tested and the survival of depurated clams was evaluated. Investigated samples were collected from Ismailia, Egypt. The initial metal concentrations were significantly higher in the whole soft tissues than in water and sediment except for Mn. After 24 h depuration, Zn, Pb, Ni, Mn, Cu, Cr and Cd were significantly reduced to 44, 23, 25, 17, 61, 41, and 75%, respectively. After three days of depuration the reduction was significant only for Cu, Cr, Co and Cd (27, 15, 23 and 52%, respectively). The...
Relationships between seminal plasma composition and sperm quality parameters of the Salmo trutta macrostigma (Dumeril, 1858) semen: with emphasis on sperm motility
Y. Bozkurt, F. Öğretmen, Ö. Kökçü, U. Erçin
Czech J. Anim. Sci., 2011, 56(8):355-364 | DOI: 10.17221/2394-CJAS
The mineral and organic composition of seminal plasma, physical spermatological parameters and their physiological relationships were investigated in Salmo trutta macrostigma. The seminal plasma contained 121.0 ± 0.37mM/l (Na+), 8.18 ± 0.03mM/l (K+), 7.23 ± 0.03 mg/dl (Ca2+), 3.19 ± 0.02 mEq/l (Mg++), 0.48 ± 0.02 g/dl total protein, 6.07 ± 0.06 mg/dl cholesterol, 6.24 ± 0.08 mg/dl triglyceride and 9.97 ± 0.39 mg/dl urea. The following physical spermatological parameters were found out: sperm volume 13.93 ± 0.84 ml, sperm motility 80.37 ± 2.36%, movement...
Stability of estimated breeding values for average daily gain in Pannon White rabbits
I. Nagy, J. Farkas, P. Gyovai, I. Radnai, Z. Szendrő
Czech J. Anim. Sci., 2011, 56(8):365-369 | DOI: 10.17221/2398-CJAS
Stability of estimated breeding values for average daily gain (ADG) between 5 and 10 weeks of age was analysed for 47 242 Pannon White rabbits, reared in 7470 litters and born between 2000 and 2008. The dataset was divided into 5 successive 5-year periods: (1) 2000-2004, (2) 2001-2005, (3) 2002-2006, (4) 2003-2007, and (5) 2004-2008. Then, after selecting the appropriate part of the pedigree for these sub-datasets, genetic parameters and breeding values were estimated for ADG using REML and BLUP methods. In the applied models sex, year-month, animal and random litter effects were considered. Estimated heritabilities for all 5 periods from 1 to 5 were...
High resolution melting as an alternative method to genotype diacylglycerol O-acyltransferase 1 (DGAT1) K232A polymorphism in cattle
A. Abdolmohammadi, H. Atashi, P. Zamani, C. Bottema
Czech J. Anim. Sci., 2011, 56(8):370-376 | DOI: 10.17221/2393-CJAS
PCR-RFLP analysis is a common method for genotyping the DGAT1 K232A polymorphism in cattle. Our purpose was to develop a high resolution melting (HRM) assay in order to genotype the polymorphic alleles. Firstly, the PCR-RFLP method was used and the 411 bp products including the DGAT1 polymorphism were digested by CfrI enzyme. Direct sequencing was performed to confirm genotypes of the K232A polymorphism for 30 samples that presented different PCR-RFLP patterns. It was determined according to sequencing results that partial enzyme digestion had occurred for some samples. A 130 bp fragment including the polymorphism...
Effect of in ovo ghrelin administration on subsequent serum insulin and glucose levels in newly-hatched chicks
A. Lotfi, H. Aghdam-Shahryar, J. Ghiasi-Ghalehkandi, H. Kaiya, N. Maheri-Sis
Czech J. Anim. Sci., 2011, 56(8):377-380 | DOI: 10.17221/2396-CJAS
Ghrelin is a regulatory peptide in glucose homeostasis in animal species. Its effect in the avian embryo is unclear. The aim of this study was to investigate the effects of in ovo ghrelin administration on serum glucose and insulin levels of hatched chicks. A total of 250 fertilized eggs were divided into 5 groups; group T1 as control (without injection), group T2 (in ovo injected with 50 ng/egg ghrelin on day 5), group T3 (in ovo injected with 100 ng/egg ghrelin on day 5), group T4 (in ovo injected with 50 ng/egg ghrelin on day 10) and group T5 (in ovo injected with 100 ng/egg ghrelin on day 10). After...