Czech Journal of Animal Science, 2011 (vol. 56), issue 4
Relationship between the insertion/deletion polymorphism within the promoter and the intron 1 sequence of the PRNP gene and milk performance traits in cattle
J. Strychalski, U. Czarnik, M. Pierzchała, C.S. Pareek
Czech J. Anim. Sci., 2011, 56(4):151-156 | DOI: 10.17221/1436-CJAS 
Current studies on the prion protein (PRNP) gene polymorphism focus primarily on the causative mutations that influence BSE susceptibility in cattle. The specific genetic structure determined by the insertion/deletion (indel) polymorphism within the 23 bp promoter sequence and the 12 bp intron 1 sequence of the PRNP gene, and its genomic location suggest that this polymorphism can be a potential QTL marker. The objective of the present study was to determine whether the indel polymorphism within the promoter sequence (23 bp) and the intron 1 sequence (12 bp) of the PRNP gene can be used as a factor differentiating...
Genetic parameters for linear type traits in Czech Holstein cattle
E. Němcová, M. Štípková, L. Zavadilová
Czech J. Anim. Sci., 2011, 56(4):157-162 | DOI: 10.17221/1435-CJAS
The objectives of this study were to estimate genetic parameters for 18 currently recorded linear conformation traits in Czech Holstein cattle. The data set consisted of 78 886 first-lactating Holstein cows. Traits, all scored on a 9-point scale, were fore udder attachment, rear udder height, udder depth, rear udder width, central ligament, teat length, front teat placement, rear teat position, stature, angularity, chest width, body depth, rump angle, rump width, rear legs rear view, rear leg set (side view), foot angle, and bone quality. Uni- and bivariate animal models were used. The models included fixed effects of herd-date of classification-classifier,...
Intra-line and inter-line genetic diversity in sire lines of the Old Kladruber horse based on microsatellite analysis of DNA
L. Vostrý, O. Kracíková, B. Hofmanová, V. Czerneková, T. Kott, J. Přibyl
Czech J. Anim. Sci., 2011, 56(4):163-175 | DOI: 10.17221/1437-CJAS
The Old Kladruber horse is the oldest original Czech horse breed - gene resource, with pedigree records spanning three centuries. Because the population is closed, there is a concern about the loss of genetic variation. The genetic diversity within and amongst sire lines was evaluated using microsatellite markers and based on pedigree information. The DNA analysis, covering 16 microsatellite loci, revealed that the genetic diversity (observed heterozygosity = 0.637, genetic diversity = 0.678 and the mean number of alleles = 8.25) for the Old Kladruber horse is consistent with other populations of horses. Inter-line genetic difference in sire lines...
Effect of missense mutation Asp298Asn in MC4R on growth and fatness traits in commercial pig crosses in the Czech Republic
V. Dvořáková, R. Stupka, M. Šprysl, J. Čítek, M. Okrouhlá, E. Kluzáková, H. Kratochvílová
Czech J. Anim. Sci., 2011, 56(4):176-180 | DOI: 10.17221/1305-CJAS
The current knowledge of factors regulating voluntary feed intake in pigs is quite limited. The objective of this study was to test the influence of the missense mutation p.Asp298Asn (AF087937:c.746G>A) of the MC4R gene on selected production traits in pig crosses. These crosses are commonly used on commercial farms in the Czech Republic. The allele frequencies of c.746G>A were as follows: G allele - 0.59 and A allele - 0.41. We detected statistically significant differences in the content of intramuscular fat in the musculus longissimus lumborum et thoracis, and a similar trend was observed in shoulder and...
Milk performance of dairy cows supplemented with rape seed oil, peanut oil, and sunflower seed oil
X.J. Dai, C. Wang, Q. Zhu
Czech J. Anim. Sci., 2011, 56(4):181-191 | DOI: 10.17221/1434-CJAS
The objective of the study was to investigate the effects of supplementing different plant oils to the basal diet on milk yield and milk composition in mid-lactating dairy cows. Forty Chinese Holstein dairy cows averaging 120 days in milk (DIM) at the start of the experiment (body weight = 580 ± 18.2 kg; milk yield = 33.0 ± 2.00 kg/day) were used in a completely randomized block design. The animals were assigned to four dietary treatments according to DIM and milk yield, and supplemented with no oil (control), 2% rapeseed oil (RSO), 2% peanut oil (PNO) and 2% sunflower seed oil (SFO). Milk yield and milk composition (fat, protein, and...
Molecular profiling of bacterial species in the geese cecum
B.Y. Liu, Z.Y. Wang, H.R. Wang, P. Hu, D. Xu, Q. Wang
Czech J. Anim. Sci., 2011, 56(4):192-203 | DOI: 10.17221/1433-CJAS
The purpose of this study was to analyse the microbial diversity in the caecum of geese using a 16S ribosomal RNA gene (rRNA) clone library approach. A total of 160 clones and 124 clones were sequenced and phylogenetically analysed from the contents and mucosa of the caecum of Yang Zhou geese, respectively. The result indicated that there was a rich variety of bacteria in the caecum contents. Forty-six operational taxonomic units (OTUs) based on a 98% similarity criterion were classified in the contents of goose caecum, as compared to 29 OTUs based on a 97% similarity criterion in the mucosa of goose caecum. The sequences were assigned to 7 and 5 groups...