Czech Journal of Animal Science, 2010 (vol. 55), issue 6

Evaluation of 11 microsatellite loci for their use in paternity testing in Yugoslav Pied cattle (YU Simmental cattle)

J. Stevanovic, Z. Stanimirovic, V. Dimitrijevic, M. Maletic

Czech J. Anim. Sci., 2010, 55(6):221-226 | DOI: 10.17221/183/2009-CJAS  

Eleven microsatellite loci (TGLA227, BM2113, TGLA53, ETH10, SPS115, TGLA126, TGLA122, INRA023, ETH3, ETH225, BM1824) were evaluated for their use in paternity testing in the Yugoslav Pied cattle (YU Simmental cattle) population in Serbia. A total of 40 animals were tested. At the 11 tested loci, a total of 91 alleles were detected. The mean number of alleles per locus was 8.273. Polymorphism information content (PIC) values ranged from 0.58 to 0.88 with the mean value of 0.72. The most informative loci were: TGLA53 (14 alleles, PIC = 0.88), TGLA227 (11 alleles, PIC = 0.82), INRA023 (11 alleles, PIC = 0.86), BM2113...

An association of C/T polymorphism in exon 2 of the bovine insulin-like growth factor 2 gene with meat production traits in Polish Holstein-Friesian cattle

L. Zwierzchowski, E. Siadkowska, J. Oprządek, K. Flisikowski, E. Dymnicki

Czech J. Anim. Sci., 2010, 55(6):227-233 | DOI: 10.17221/94/2009-CJAS  

The effect of the insulin-like growth factor 2 (IGF2) gene polymorphism - a g.292C>T transition in exon 2 on feed uptake and conversion, growth rates, and meat production traits in Polish Holstein-Friesian cattle was investigated. A total of 291 young bulls were genotyped using restriction fragment length polymorphism (RFLP-BsrI). Animals were slaughtered at the age of 11 or 15 months and carcass traits were examined. At the age of 11 months the association was shown of the CC genotype with higher cold carcass weight, daily gain and meat content (%) in valuable cuts. At 15 months of age the CC genotype bulls showed higher live...

A comparison of the follicular dynamics in heifers of the Czech Fleckvieh and Holstein breeds

J. Šichtař, R. Tolman, R. Rajmon, P. Klabanová, P. Berka, J. Volek

Czech J. Anim. Sci., 2010, 55(6):234-242 | DOI: 10.17221/242/2009-CJAS  

Differences in follicular development and repeatability of follicular growth pattern among Czech Fleckvieh (n = 20) and Holstein (n = 23) heifers were investigated. Follicular dynamics was evaluated by daily sonographic scanning during three interovulatory intervals. The mean duration of the interovulatory interval was 20.66 ± 0.32 days, no differences between breeds were observed. The proportion of the non-alterna-ting pattern was nearly the same as that of the alternating pattern (54% and 46%, respectively). The majority of IOIs ≤ 21 days were of the 2-wave pattern (71%) whereas only 29% of them were of the 3-wave pattern....

The influence of housing systems on the air quality and bacterial eggshell contamination of table eggs

M. Vučemilo, B. Vinković, K. Matković, I. Štoković, S. Jakšić, S. Radović, K. Granić, Đ. Stubičan

Czech J. Anim. Sci., 2010, 55(6):243-249 | DOI: 10.17221/64/2009-CJAS  

This paper compares two different housing systems for laying hens producing table eggs, namely a conventional cage system and an aviary, during three summer months, starting from the 20th week of the production cycle. Research was focused on airborne bacteria, fungi and dust levels and on the bacterial eggshell contamination. Levels of airborne bacteria determined in the aviary system were many times higher and ranged from 6.2 × 104 CFU/m3 to 8.9 × 104 CFU/m3, and the levels of airborne fungi ranged from 1.6 × 104 to 1.9 × 104 CFU/m3, while the levels of airborne bacteria and fungi determined in...

Steroid effects on the in vitro LH secretion from pituitary cells of sexually immature carp (Cyprinus carpio L.) under the influence of naltrexone and morphine

M. Sokolowska-Mikolajczyk, M. Socha, P. Epler

Czech J. Anim. Sci., 2010, 55(6):250-257 | DOI: 10.17221/131/2009-CJAS  

Dispersed cells from sexually immature carp (footlings) pituitaries were exposed to estradiol (E2) or testosterone (T) (both at 3 × 10-8 M) in the presence of opioid receptor antagonist naltrexone (10-8 or 10-6 M) and/or agonist - morphine (10-8 or 10-6 M). Naltrexone alone at 10-6 M increased the LH level as compared with the control. Morphine (10-8 or 10-6 M), T and E2 had no influence on LH levels. The combination of T with naltrexone (10-8 M) stimulated LH release if compared with the control or with T alone. Morphine (both concentrations)...

The use of indirect methods for the prediction of lucerne quality in the first cut under the conditions of Central Europe

J. Hakl, J. Šantrůček, P. Fuksa, L. Krajíc

Czech J. Anim. Sci., 2010, 55(6):258-265 | DOI: 10.17221/86/2009-CJAS  

The goal of this study was to compare the growing degree-days (GDD) and predictive equations for alfalfa quality (PEAQ) for the prediction of lucerne quality and to test their accuracy and suitability in the first cut period in Central Bohemia. Several additional stand parameters were verified in order to increase the accuracy of the quality estimate under these environmental conditions. In 2004-2007, the measurement and sampling were repeatedly realized from the late vegetative to the early bloom stage in six replications. For the GDD model across the years, the obtained R2 for NDF, ADF and CP were 0.40, 0.57 and 0.65, respectively. It...