Czech Journal of Animal Science, 2009 (vol. 54), issue 6
Effect of rumen-protected methionine, lysine or both on milk production and plasma amino acids of high-yielding dairy cows
J. Třináctý, L. Křížová, M. Richter, V. Černý, J. Říha
Czech J. Anim. Sci., 2009, 54(6):239-248 | DOI: 10.17221/1730-CJAS 
The objective of this study was to determine the effect of supplemental lysine (Lys), methionine (Met) or both amino acids added in the form of rumen-protected (RP) tablets with copolymer coating to a diet of dairy cows on yield and composition of milk and concentration of plasma amino acids (AA). The experiment was carried out on four high-yielding lactating Holstein cows with average milk production of 33.5 kg/day in the form of Latin square design. The four treatments were as follows: C - control without AA supplementation, L - control plus supplement of RP Lys, M - control plus supplement of RP Met and ML - control plus supplement of RP Met and...
Economic weights of production and functional traits in dairy cattle under a direct subsidy regime
Z. Krupová, J. Huba, J. Daňo, E. Krupa, M. Oravcová, D. Peškovičová
Czech J. Anim. Sci., 2009, 54(6):249-259 | DOI: 10.17221/1731-CJAS
: Economic weights (EW) for the Slovak dairy production system were calculated in Holstein, Slovak Simmental, and Slovak Pinzgau breeds under a direct support regime using a bio-economic approach. EW were calculated for three scenarios (A: without agricultural subsidies; B: agricultural subsidies included in revenues and C: agricultural subsidies included in feeding costs). Quota-free milk production was assumed in all scenarios. The base price of milk was corrected according to real fat, protein and somatic cell content. Under the given economic and production conditions, only the Holstein system in scenario A was economically profitable. All simulated...
Effect of carbohydrates in grass silage-based diets on in sacco ruminal degradability of barley (Hordeum vulgare L. cv. Lomerit) grain ground to different particle sizes
B. Niwińska
Czech J. Anim. Sci., 2009, 54(6):260-269 | DOI: 10.17221/1732-CJAS
The effect of carbohydrates included in grass silage-based diets on in sacco degradability of dry matter (DM), crude protein (CP), neutral detergent fibre (NDF) and cellulose (CE) of barley (Hordeum vulgare L. cv. Lomerit) grain ground to different particle sizes was studied using 3 ruminally cannulated cows. The measurements were carried out as a 3 × 2 Latin square design with treatments being carbohydrates in the concentrate portion of diets (starch in diet I vs. pectin in diet II vs. sucrose in diet III) and particle size of ground barley grain (1.0 vs. 5.7 mm). The diet synchronization index between N and energy supply during the...
Analysis of rabbit cell surface (CD) antigens by means of cross-reactive monoclonal antibodies with specificity for cattle CD antigens
M. Simon, J. Antalíková, Ľ. Horovská, J. Jankovičová, K. Fábryová, S. Hluchý, P. Chrenek, V. Tančin
Czech J. Anim. Sci., 2009, 54(6):270-276 | DOI: 10.17221/1728-CJAS
Studies that involved testing monoclonal antibodies (mAbs) for cross-species reactivity proved to be efficient for the identification of previously unrecognized antigens in a number of different species. Twenty-six mAbs specific to different bovine CD (cluster defined) antigens (CD9, CD18, CD45R, CD41/61, CD62L, MHC class I and bovine IgG light chain molecule) were assayed for reactivity with rabbit peripheral blood leukocytes. Four of the mAbs recognizing CD9 and CD41/61 were reactive with rabbit platelets or granulocytes. These were investigated further by immunoblotting and immunohistochemical staining. The study identified CD9 and CD41/61 molecules...
Effects of lucerne particle size and source of dietary carbohydrates on in situ degradation and ruminal variables in sheep
A. Asadi Alamouti, G.R. Ghorbani, M. Alikhani, H.R. Rahmani, A. Teimouri Yansari, K.H. Südekum
Czech J. Anim. Sci., 2009, 54(6):277-285 | DOI: 10.17221/1729-CJAS
The effects of altering forage particle size and source of rapidly degradable carbohydrates on in situ degradation and ruminal variables were studied in four Iranian male sheep. The study was designed as a Latin square with a 2 × 2 factorial arrangement of treatments including two carbohydrate sources (pelleted beet pulp vs. maize- and barley-based concentrate) and two lucerne particle sizes (2.38 vs. 0.94 mm). Kinetics of disappearance of lucerne, concentrates and mixed samples was studied in situ. Among feed samples, the degradation rate constant of lucerne dry matter was higher (P < 0.02) and disappearance of lucerne neutral...
The effect of water temperature on the number of moults and growth of juvenile signal crayfish Pacifastacus leniusculus Dana
P. Kozák, M. Buřič, J. Kanta, A. Kouba, P. Hamr, T. Policar
Czech J. Anim. Sci., 2009, 54(6):286-292 | DOI: 10.17221/1727-CJAS
The growth rate, frequency of moulting, and intermoult intervals of juvenile Pacifastacus leniusculus were studied under experimental conditions over a period of 3 months. Juveniles were reared individually in small boxes at two different temperatures: 14.31 ± 0.64°C (cold water) and 20.54 ± 0.69°C (warm water). Although the average weight and length of consecutive stages were similar at both temperatures, juveniles in warm water attained more moults. Within the age group juveniles achieved a higher length and weight in warm water than in cold water. Three month-old juveniles reached 147 mg and 18.5 mm in cold water at the 6th...