Czech Journal of Animal Science, 2007 (vol. 52), issue 4

Joint effects of CSN3 and LGB genotypes and their relation to breeding values of milk production parameters in Czech Fleckvieh

A. Matějíček, J. Matějíčková, E. Němcová, O.M. Jandurová, M. Štípková, J. Bouška, J. Frelich

Czech J. Anim. Sci., 2007, 52(4):83-87 | DOI: 10.17221/2273-CJAS  

The objective of this study was to estimate the joint effects of CSN3 and LGB genotypes on breeding values of milk production parameters. CSN3 (kappa-casein) and LGB (beta-lactoglobulin) genotypes of 120 Czech Fleckvieh sires were detected using the PCR-RFLP method. Breeding values of sires were obtained from the Official Database of Progeny Testing. Ten genotype combinations were detected. Genotypes ABAB (25.0%), ABAA (13.3%) and ABBB (13.3%) were the most frequent. Significant effects of genotype combinations on breeding values for fat and protein content were found. The highest breeding values for...

Immunohistochemical localization of adhesion molecules (CD62 and CD18) in the mammary gland of dairy cows

M. Simon, S. Hluchý, Ľ. Horovská, J. Antalíková, J. Čuboň

Czech J. Anim. Sci., 2007, 52(4):88-95 | DOI: 10.17221/2272-CJAS  

Localization of the L-selectin (CD62L) and β2-integrin (CD18) bearing cells in different tissues of the bovine mammary gland was examined. Five dairy cows of Holstein-Friesian breed in the middle of their second and third lactation cycle were used in the study. Blood, milk and udder tissue samples were collected from each cow to estimate the milk somatic cell count (SCC) and bacteriological infection of the mammary gland. The expression of CD62L and CD18 on blood cells, milk cells and parenchymal tissues of udder, Fürstenberg's rosette and the transverse section of the central part of the teat was tested. In the mammary gland quarters the value...

Acid-base homeostasis of blood and pH of abomasum in calves fed non-acidified and acidified milk replacer

V. Vajda, I. Maskaľová, A. Tesfaye

Czech J. Anim. Sci., 2007, 52(4):96-102 | DOI: 10.17221/2271-CJAS  

Experiments were conducted on calves divided into three groups, 6 animals in each, to determine the influence of the intake of non-acidified and acidified milk replacer on the level of acid-base homeostasis in venous blood. The milk replacer was supplied at a dose of 700 g dry matter in 8 l of the liquid in two feedings. The milk replacer was acidified by adding formic acid to ensure the concentration of 0.2% in milk replacer. Venous blood was collected before feeding and 1, 2, 4, and 6 hours after feeding. The samples were analyzed for blood pH, actual bicarbonate HCO3- (mmol/l), base excess BE (mmol/l), partial pressure of carbon...

Pattern of fibre type distribution within muscle fascicles of pigs (Sus scrofa domestica)

D. Škorjanc, A. Šalehar, I. Eržen, M. Kastelic

Czech J. Anim. Sci., 2007, 52(4):103-109 | DOI: 10.17221/2270-CJAS  

In order to determine breed differences in the fascicle structure of skeletal muscles, longissimus dorsi muscles (LD) and semimembranous muscles (SM), Duroc (D), Large White (LW), German Landrace (GL) and Swedish Landrace (SL) boars were analyzed. Fibre type delineation was based on the method of myofibrillar ATPase staining. The fibres in a fascicle were divided into layers according to their relation to the perimysium. In each layer, the proportions of fibre types I, IIA and IIB were determined and normalized according to the fibre type proportion in the whole fascicle. Generally, a high proportion of IIB fibres on the periphery, a prevalence of...

The effects of housing systems and age of hens on the weight and cholesterol concentration of the egg

Ľ. Zemková, J. Simeonovová, M. Lichovníková, K. Somerlíková

Czech J. Anim. Sci., 2007, 52(4):110-115 | DOI: 10.17221/2269-CJAS  

The effects of laying hen age and the housing of laying hens in conventional cages, enriched cages, litter system and outdoor system on egg weight and yolk and egg cholesterol concentration were studied. ISA brown laying hens were used in the experiment. The characteristics were measured at 39, 50, 59, 68 and 75 weeks of age. Fifteen eggs were always collected from each housing system. In total, 300 eggs were analyzed. There were not any significantly different results in the eggs from the hens living in the different housing systems or from the age of laying hens on the weight of eggs. But the housing system and the age of hens had a significant (P...