Czech Journal of Animal Science, 2005 (vol. 50), issue 6
Microsurgical nuclear transfer by intraooplasmic karyoplast injection as an alternative embryo reconstruction method in somatic cloning of pigs and other mammal species; application value of the method and its technical advantages: a review
M. Samiec, M. Skrzyszowska
Czech J. Anim. Sci., 2005, 50(6):235-242 | DOI: 10.17221/4163-CJAS 
The introduction of cell nuclei into enucleated recipient cells, beyond enucleation, is the most significant stage of somatic cloning procedure. Microsurgical transfer of somatic nuclei can be an alternative method of clonal nuclear-cytoplasmic hybrid reconstruction towards cell fusion induced in the electric field, not only from the aspect of molecular mechanisms of nuclear chromatin rearrangement, advantageously influencing epigenetic reprogramming and structural remodelling of exogenous genetic material but also because in recent studies on pig cloning it was proved that the effectiveness of piezo-driven microinjection of ear-derived fibroblast...
Development of testicular dimensions and size, and their relationship to age and body weight in growing Kivircik (Western Thrace) ram lambs
M. Koyuncu, S. Kara Uzun, S. Ozis, S. Duru
Czech J. Anim. Sci., 2005, 50(6):243-248 | DOI: 10.17221/4164-CJAS
Forty-seven Kivircik ram lambs, aged 2 months, raised and managed under an intensive system were used to measure the development of testis diameter and length, and scrotum circumference, length and volume, and their association with the development of body growth. The effects of age and body weight of lambs, type of birth and dam age on the development of the five testicular parameters from weaning at 2-6 months of age were investigated. In ram lambs, the respective least-squares means of testis measurements: testis diameter, testis length, scrotum circumference, scrotum length and scrotum volume were 2.55 ± 0.099 cm, 5.83 ± 0.165 cm,...
Effect of the oestrogen receptor (ESR) gene on reproductive traits of Large White, White Meaty and Landrace pigs
R. Omelka, M. Bauerová, J. Mlynek, B. Buchová, D. Peškovičová, J. Bulla
Czech J. Anim. Sci., 2005, 50(6):249-253 | DOI: 10.17221/4165-CJAS
We investigated the effect of oestrogen receptor (ESR) gene on total number of born (TNB), number of born alive (NBA) and number of weaned (NW) piglets in Large White (LW), White Meaty (WM) and Landrace (L) sows from six Slovak breeding farms. Detection of ESR (Pvu II) genotypes was performed by the PCR-RFLP method. The frequencies of favourable B allele in LW, WM and L were 0.33, 0.25 and 0.08, respectively. We found a highly significant effect of ESR locus on NW (P ≤ 0.01) in LW, however, we observed a negative effect of BB genotype on the trait. In WM, a positive association of B...
Sub-indexes for bulls ofHolsteinbreed in the Czech Republic
P. Šafus, M. Štípková, L. Stádník, J. Přibyl, V. Čermák
Czech J. Anim. Sci., 2005, 50(6):254-265 | DOI: 10.17221/4166-CJAS
Sub-indexes were constructed for bulls of the Holstein breed according to groups of production traits - production index for milk (IPH), sub-indexes for reproduction (IRH), longevity (IDH), health resistance (IOH) and fitness (IFH). Every index for selection for a group of traits applies all available information - breeding values for traits of milk performance, fertility and linear type trait classification. The sub-indexes were compared with the total index (SIH). The accuracy of total selection index (SIH) was 69.30%, the accuracies of the other indexes were as follows: IPH 83.32%, IRH 56.04%, IDH 9.80%, IOH 15.57% and IFH 9.86%. All of the indexes...
Breeding value evaluation in Polish fur animals: Factors affecting pelt prices in the international trading system
H. Wierzbicki
Czech J. Anim. Sci., 2005, 50(6):266-272 | DOI: 10.17221/4167-CJAS
The aim of this study was to determine the contribution of pelt character traits to the price the skins could reach at an auction house. The paper also presents an analysis of trends showing the changes in the contribution of pelt character traits to the pelt price over 4 seasons. 10 177 records of arctic fox pelts sold at the Helsinki Auction House in the seasons 2000-2004 were analysed. The pelts were graded and sorted according to their character traits: size, quality, colour clarity and colour darkness. The significance of the pelt character traits and their effects on the pelt price were tested using the GLM procedure and two linear models (Model...
The effect of the administration of cellulose and fructans with different degree of polymerization to rats on caecal fermentation and biochemical indicators in the serum
J. Juśkiewicz, Z. Zduńczyk, M. Wróblewska
Czech J. Anim. Sci., 2005, 50(6):273-280 | DOI: 10.17221/4168-CJAS
Research was conducted to determine the physiological effect of substituting 5% of maize starch with cellulose, inulin and oligofructose in the diet of rats. The feeding of cellulose and fructans did not influence the food intake and weight gains of animals. The addition of oligofructose and inulin induced an enlargement of the caecum (wall and digesta). Dry matter and pH of caecal digesta were significantly decreased in rats receiving fructan preparations compared to the cellulose and starch groups. Inulin and oligofructose caused a significant increase in the SCFA content produced in the whole caecum (calculated per 100 g BW). Cellulose as well as...
In situ evaluation of ruminal degradability and intestinal digestibility of extruded soybeans
W. Nowak, S. Michalak, S. Wylegała
Czech J. Anim. Sci., 2005, 50(6):281-287 | DOI: 10.17221/4169-CJAS
Two ruminally cannulated Jersey cows were used to determine in situ ruminal degradability and intestinal digestibility of raw (E0) soybeans and soybeans extruded at 145°C (E145), 155°C (E155) and 165°C (E165). The degradation of dry matter (DM) and crude protein (CP) was estimated using nylon bags suspended in rumen for 2, 4, 8, 12, 16, 24 and 48 h calculating the effective ruminal degradabilities (ERD) for an assumed outflow rate of 0.06/h. Four extra sample bags were incubated for 12 h to determine CP digestibility using the mobile nylon bag method. Cows were fed a ration of 30% concentrate and 70% meadow hay. Soybean extrusion at all temperatures...