Czech Journal of Animal Science, 2005 (vol. 50), issue 5

Molecular conditions of the cell nucleus remodelling/reprogramming process and nuclear-transferred embryo development in the intraooplasmic karyoplast injection technique: a review

M. Samiec, M. Skrzyszowska

Czech J. Anim. Sci., 2005, 50(5):185-195 | DOI: 10.17221/4142-CJAS  

The introduction of cell nuclei into enucleated recipient cells, beyond enucleation, is the most significant stage of somatic cloning procedure. Microsurgical transfer of somatic nuclei can be an alternative method of clonal nuclear-cytoplasmic hybrid reconstruction towards cell fusion induced in the electric field, not only from the aspect of molecular mechanisms of nuclear chromatin rearrangement, advantageously influencing epigenetic reprogramming and structural remodelling of exogenous genetic material, but also because it was proved in recent studies on pig cloning that the effectiveness of piezo-driven microinjection of ear-derived fibroblast...

Non-contact thermometry in the milking stopping control system

I. Karas, R. Gálik

Czech J. Anim. Sci., 2005, 50(5):196-200 | DOI: 10.17221/4148-CJAS  

The paper deals with the detection of "idle milking" times for individual quarters of the udder in a group of dairy cows (randomly selected) in a parallel 2 × 12 milking parlour. A non-contact laser thermometer Raynger ST-6 was used to measure temperatures of the inner surfaces of liners instantly after milking. In a group of 12 dairy cows, the minimum liner temperature after milking was 15.3°C, the maximum temperature was 28.9°C. It follows from the regression correlation that an increase in the cooling time by 1 second decreases the temperature of the liner inner surface by 0.0324°C. On average, fore left quarters were milked idle 2.55 min, fore...

Effect of different forms of bacterial inoculants on the fermentation process of ensiled crushed maize moisture grains

P. Doležal, L. Zeman

Czech J. Anim. Sci., 2005, 50(5):201-207 | DOI: 10.17221/4149-CJAS  

Crushed high-moisture maize grains were ensiled in laboratory silos with different (water-soluble and granulated) forms of biological inoculants. The characteristics of the quality of silage fermentation process were analysed. The water-soluble inoculant product (WSI) Bonsilage and a microgranulated product (MGI) were compared with untreated control (UC). The water-soluble inoculant increased (P < 0.01) acetic acid (AA) production and the ratio LA/AA, decreased pH value, and inhibited production of ammonia (P < 0.05). The best results and the highest quality of preserved silage were obtained when the WSI preparation was used....

Effect of dietary vitamin C supplement on reproductive performance of aviary pheasants

S. Nowaczewski, H. Kontecka

Czech J. Anim. Sci., 2005, 50(5):208-212 | DOI: 10.17221/4150-CJAS  

The effects of three doses of vitamin C (100, 200 and 300 mg/kg) added to the feed of reproductive pheasants on egg production, egg fertility and parameters of hatchability were investigated. In experiments I (1999) and II (2000) birds were kept in outdoor aviaries. In 2000, air temperatures recorded on the farm during the experiment were high (25-30oC), differing considerably from those recorded in the preceding years. In experiment I no statistically significant differences were shown between the control pheasants and those supplemented with 100 and 200 mg/kg vitamin C in terms of egg production, egg fertility and parameters of hatchability....

Chemical composition of flesh of diploid and triploid population of tench (Tinca tinca, Linnaeus 1758)

H. Buchtová, L. Vorlová, Z. Svobodová, M. Flajšhans

Czech J. Anim. Sci., 2005, 50(5):213-219 | DOI: 10.17221/4152-CJAS  

The objective of this study was to determine differences in relevant parameters of the basic chemical composition of flesh (descriptors: dry matter - DM, crude proteins - CP, net proteins - NP and net muscle proteins - NMP, fat - F, ash - A) of communal stock of diploid (2n) and triploid (3n) tench (Tinca tinca L.) of the same origin, in relation to sex (F: female vs. M: male) and age (T3: 36 months, after overwintering vs. T3+: 42 months, after one summer in the pond). Altogether 137 siblings of tench were studied. The basic chemical composition of flesh of 2n and 3n tench of T3...

Production value, chemical composition and colour of fillets of the reciprocal hybrid of Siberian sturgeon with green sturgeon (Acipenser baeri Br × (Acipenser baeri × Acipenser medirostrisAyres)

B. Jankowska, R. Kolman, M. Szczepkowski, T. Żmijewski

Czech J. Anim. Sci., 2005, 50(5):220-225 | DOI: 10.17221/4160-CJAS  

Fillet yield, colour, chemical composition and fatty acid composition in the reciprocal hybrid (SSZ) of Siberian sturgeon with its crossbred with green sturgeon were analysed. It was found that fillet yield in relation to the weight of gutted and deheaded carcass did not deviate from the parent forms. SSZ does not keep the salmon-pink colour of tissue, which is typical of the father form. Its tissue contains more (P ≤ 0.01) protein and less (P ≤ 0.01) water than the parental tissue as well as less (P ≤ 0.01) fat than the father's tissue. The percentage of MUFA in tissue lipids corresponds to their proportion in the...

Associations of stockpersons' personalities and attitudes with performance of dairy cattle herds

J. L. Panamá Arias, M. Špinka

Czech J. Anim. Sci., 2005, 50(5):226-234 | DOI: 10.17221/4162-CJAS  

We collected questionnaires from 128 stockpersons on 31 Czech dairy farms with the aim to examine three questions: (i) whether the stockpersons' personality profiles, their attitudes and beliefs about cows, and their age/experience were related to farm performance indicators such as milk yield per standardised lactation, culling of cows or costs of veterinary treatments; (ii) whether stockpersons' personalities and attitudes were related to their age, gender and duration of their experience with cattle; and, (iii) how the personality traits of stockpersons differed from the same traits in the general Czech population. The NEO Big Five Personality Inventory...