Czech Journal of Animal Science, 2004 (vol. 49), issue 3
In vitro aging of porcine oocytes
I. Petrová, M. Sedmíková, E. Chmelíková, D. ©vestková, R. Rajmon
Czech J. Anim. Sci., 2004, 49(3):93-98 | DOI: 10.17221/4285-CJAS 
Porcine oocytes matured in vitro develop in various ways if they are further cultivated. In our studies these oocytes were cultivated for 1 to 5 days (in vitro aging). During the 1st day of aging, most of them remained at the stage of metaphase II (98%). Then many oocytes underwent the spontaneous parthenogenetic activation. The portion of activated oocytes reached its peak after 2 or 3 days of aging in vitro (39 or 45%). The portion of fragmented oocytes peaked at the same time (28%). During subsequent aging in vitro (i.e. day 4 or 5 of aging), the portion of lysed oocytes significantly increased (30 or 37%). The...
A relationship between the PCR-RFLP polymorphism in porcine MYOG, MYOD1 and MYF5 genes and microstructural characteristics of m. longissimus lumborum in Pietrain × (Polish Large White × Polish Landrace) crosses
D. Kłosowska, J. Kurył, G. Elminowska-Wenda, W. Kapela, K. Walasik, M. Pierzcha, D. Cie¶lak, J. Bogucka
Czech J. Anim. Sci., 2004, 49(3):99-107 | DOI: 10.17221/4286-CJAS
Muscle fibre formation takes place during embryonic development and is regulated by the MyoD gene family, which consists of four genes, MYOD1, myogenin, MYF5 and MRF4. A relationship was studied between MYOD1, myogenin and MYF5 genotypes and microstructural characteristics of the m. longissimus lumborum in pigs - crosses: Pietrain × (Polish Large White × Polish Landrace). The data included 115 unrelated animals slaughtered at about 105 kg live body weight. Within 45 min after exsanguination, samples were taken from the m. longissimus lumborum, frozen in liquid nitrogen and later...
Implications of b-glucanase and pentosanase enzymes in low-energy low-protein barley and wheat based broiler diets
N. Senkoylu, H. Akyurek, Samli HE
Czech J. Anim. Sci., 2004, 49(3):108-114 | DOI: 10.17221/4287-CJAS
This study was conducted to test the effects of a commercial enzyme (with beta-glucanase and pentosanase activities) supplemented into low-protein low-energy barley and wheat based broiler diets on broiler performance. The enzyme was added at 500 g/ton into broiler grower and finisher diets consisting of mainly wheat at 76%, 85% or barley 67%, 75%, respectively. Four dietary treatments were wheat, wheat + enzyme, barley, barley + enzyme. Each treatment had six replications. This experiment was planned according to a completely randomised design by placing ten 14-day-old mixed male and female chicks into one experimental cage unit with wire floor. Cobb...
The use of video image analysis for fat content estimation
P. Pipek, J. Jeleníková, L. Sarnovský
Czech J. Anim. Sci., 2004, 49(3):115-120 | DOI: 10.17221/4288-CJAS
The composition of selected cuts of cattle carcasses was determined in connection with the search for new methods of carcass classification. The content of adipose tissue and intramuscular fat in the cross-section of beef loin was estimated. A total of 79 samples was taken for investigations in a broad range of cattle category. The classical extraction method in Soxhlet extractor was compared with video image analysis, which measured the ratio of muscle to fat areas. The size and the shape of the musculus longissimus lumborum et thoracis (MLLT) and its ratio in the loin cross-section was also estimated. A good correlation (r = 0.99,...
Fish Communities in the Poodří Protected Landscape Area (the Odra River basin)
B. Lojkásek, S. Lusk, K. Hala, V. Lusková
Czech J. Anim. Sci., 2004, 49(3):121-130 | DOI: 10.17221/4289-CJAS
The Odra River, 55.2 kmof its length, is winding through the Poodří Protected Landscape Area, which has total area of 81,5 km2. More than 80% of its stream has retained the natural character of its riverbed and the hydrological regime with regular floods. Ichthyologic research was carried out in the period 1997-2001. It took place in 7 localities comprising 3 localities situated in main tributaries and 4 localities of former river arms. Gill nets and electrofishing were used for the research on limnetic biotopes. A total of 22 fish species in the streams (Odra, Lubina, Ondřejnice, and Bílovka Rivers) and 4 other species in stagnant water...