Czech Journal of Animal Science - Latest articles
Results 31 to 60 of 99:
Effects of two protein levels on the performance of chicken males with different growth intensities
Jan Tyl, Eva Tùmová, Darina Chodová
Czech J. Anim. Sci., 2025, 70(5):194-201 | DOI: 10.17221/44/2025-CJAS 
At present, genetic selection programs produce chicken genotypes with different growth intensities, which might have variable requirements for dietary protein. The objective of this study was to compare the response of three different genotypes to two levels of crude protein in feed mixtures. Cockerels of fast-growing Ross 308, medium-growing Hubbard JA 757 and slow-growing ISA Dual chickens were used in the study. Each genotype was fed diets that differed in protein level: the control group (C) received commercial feed, and the experimental group was fed a diet with a 6% lower protein content (LP). The daily weight gain (DWG) and feed conversion ratio...
Different particle sizes of laying hens diets and it’s effect on blood biochemical parameters, ileal digesta viscosity and nitrogen retention
Ondøej ©»astník, Dana Zále¹áková, Jakub Novotný, Vojtìch Kumbár, Andrea Roztoèilová, Eva Mrkvicová, Leo¹ Pavlata
Czech J. Anim. Sci., 2025, 70(5):183-193 | DOI: 10.17221/11/2025-CJAS
The aim of this work was to evaluate the effect of different particle sizes of hens diets on blood biochemical parameters, ileal digesta viscosity and nitrogen retention. In the experimental part, the effects of different physical structure were investigated on two groups of laying hens of the Bovans Brown hybrid from 76 to 80 weeks of age. A finely ground mash diet [geometric mean diameter (GMD), 632 µm] and a coarsely ground mash diet (GMD, 1 258 µm) with the equal nutritional content were used. In the experiment, the particle sizes of the feed mixtures were analysed and compared with the particle size of unaccepted feed residues using...
Mesobiliverdin IXα-enriched microalgae feed improves gut health in weaned piglets under LPS challenge
Tz-Shian Liao, Pei-En Chang, Yi-Ting Chen, Ching-Yi Chen, Cheng-Wei Tom Chang, Jon YutakaTakemoto, Yuan-Yu Lin
Czech J. Anim. Sci., 2025, 70(4):139-146 | DOI: 10.17221/159/2024-CJAS
This study aimed to evaluate the effect of mesobiliverdin IXα-enriched microalgae (MBV-SP) on growth, diarrhoea prevalence, intestinal morphology, antioxidant capacity, and inflammatory cytokines in lipopolysaccharide (LPS)-induced enteritis in weaned piglets. Seventy-two 28-day-old piglets were randomly divided into four groups: control (NC), LPS, tylosin, and MBV-SP, with saline/LPS (100 µg/kg) administered intraperitoneally on day 14. Results showed reduced mortality and diarrhoea rates in the tylosin + LPS and MBV-SP + LPS groups compared to the LPS group. Histological analysis revealed improved villus morphology and decreased crypt...
Impact of microclimatic conditions on sperm production in Czech Holstein bulls: A correlational study
Filipp Georgijeviè Savvulidi, Jaromír Ducháèek, Martin Ptáèek, Matú¹ Ga¹parík, Veronika Kováøová, Radim Codl, Ludìk Stádník
Czech J. Anim. Sci., 2025, 70(4):121-129 | DOI: 10.17221/21/2025-CJAS
In this study, the influence of microclimatic conditions in different seasons of the year (including the hottest months) on the quantitative and qualitative characteristics of ejaculates of Czech Holstein bulls was assessed. Bulls were kept in the facility with no forced ventilation or air conditioning. To determine the influence, the temperature-humidity index (THI) was calculated based on the microclimatic parameters measured directly in the facility with the animals. Bull sperm was obtained using an artificial vagina on five occasions throughout the year, in different seasons of the year including the hottest months. Semen was assessed for...
Effects of low-protein diet supplemented with exogenous protease on growth performance and intestinal health of broiler chickens
Xin Hao, Guifeng Zhang, Yilin Ge, Jie Wang, Weiren Yang, Lijie Yang
Czech J. Anim. Sci., 2025, 70(4):147-159 | DOI: 10.17221/18/2025-CJAS
This experiment was conducted to study the effects of low-protein diet supplemented with exogenous protease on performance and intestinal health of broilers. A total of 560 one-day-old male Arbor Acres broiler chickens were randomly divided into 5 treatments with 8 replicates (12 birds per replicate) in a completely randomised design. The treatments were as follows: (1) maize-soybean meal basal diet (CON); (2) negative control with crude protein being 1% lower than in CON (NCON); (3) NCON + 12 000 U/kg coated alkaline protease (NCAP); (4) NCON + 16 000 U/kg alkaline protease (NAP); (5) NCON + 16 000 U/kg keratinase (NKA). The remaining 80 birds (10 replicates,...
Relationships among clinical mastitis test-day records, somatic cell counts, and linear udder conformation traits in Czech dairy cows
Jan Vaøeka, Ludmila Zavadilová, Matú¹ Ga¹parík, Miloslava ©típková, Lubo¹ Vostrý
Czech J. Anim. Sci., 2025, 70(4):130-138 | DOI: 10.17221/12/2025-CJAS
This study evaluated test-day records of clinical mastitis (CM), somatic cell count, and nine udder conformation traits. Somatic cell count was log-transformed into somatic cell score (SCS) in 10 periods, each 30 days long and overall, for the first lactation. CM is a complex disease closely connected with somatic cell count. The optimum udder conformation traits significantly affect dairy cattle health. The CM binary trait was monitored in seven periods throughout lactation, each 50 days long, and for the whole lactation. A logistic regression model was used to estimate the risk of CM. The model included a fixed effect of herd-year-season, age...
Whole-genome resequencing data reveal the genetic diversity of local chickens in southern Zhejiang and surrounding areas in China
Yawen Zhang, Bo Zhang, Ziwei Li, Fengxiang Hou, Yan Zhao, Junjie Jin, Bin Song, Wenjie Gu
Czech J. Anim. Sci., 2025, 70(3):113-120 | DOI: 10.17221/186/2024-CJAS
China’s local chicken breeds, especially those in the Zhejiang Province, are rich in genetic diversity owing to environmental heterogeneity and complex ecosystems. Distinctive local breeds have emerged through long-term natural selection and domestication. We investigated the genetic diversity and population structure of local chickens in southern Zhejiang and surrounding areas using whole-genome resequencing of 129 chickens from seven populations. A total of 1.8 terabytes of raw data was obtained, and 4 802 728 single nucleotide polymorphisms were detected. The Xianju chicken population exhibited the highest genetic diversity, while Yandang...
Association between conventional semen variables and sperm freezability in rams
Aizhan Makhanbetova, Filipp Georgijeviè Savvulidi, Martin Ptáèek, Lucie Langerová, Beybit Kulataev, Nurlan Malmakov
Czech J. Anim. Sci., 2025, 70(3):93-101 | DOI: 10.17221/185/2024-CJAS
Assessments of routine ejaculated samples were used to evaluate the conventional semen variables (sperm motility, semen volume, sperm concentration) as initial decision-making criteria to freeze or not to freeze. However, the association of these attributes to the cryotolerance of ram sperm has not been adequately studied yet. The aim of this study was therefore to analyse if the semen volume, sperm motility and concentration can be used to predict post-thaw ram sperm motility. Additionally, in the present study we tested if the order of ejaculate sampling (first versus second, within a single collection session) and the breed of rams influenced...
LGALS3 promotes the proliferation of Liaoning Cashmere goat skin fibroblasts by regulating the expression of PIEZO1
Mei Jin, Weiyu Fan, Jinxu Xu
Czech J. Anim. Sci., 2025, 70(3):102-112 | DOI: 10.17221/72/2024-CJAS
Long non-coding RNA (lncRNA) plays a role in the reconstruction of hair follicles and in the formation and growth of cashmere fibres. One cashmere growth-associated lncRNA was successfully screened out at the early stage in the laboratory, which was named LncRNA MTC. In this study, the binding of galectin 3 (LGALS3) to LncRNA MTC was examined through RNA immunoprecipitation (RIP) in skin fibroblasts of Liaoning Cashmere goats. The pLenti-LGALS3-His-IRES-EGFP lentiviral overexpression vector was built, and the result of CCK8 indicated that the overexpression of LGALS3 significantly enhanced the viability of skin fibroblasts (P < 0.05)....
Analysis of the mare breeding population of Haflinger breed in the Czech Republic
Michaela Kuli¹»áková, Eva Sobotková, Radek Filipèík
Czech J. Anim. Sci., 2025, 70(3):83-92 | DOI: 10.17221/8/2025-CJAS
The aim of this study was to analyse the conformation traits and body dimensions of Haflinger mares registered in the Czech Republic studbook. The study focused on the effects of age at studbook entry, year of birth, proportion of Arabian genes, and country of origin on various parameters. Data were collected from 825 mares, including scores obtained during the studbook entry evaluation and body measurements such as withers height measured with a stick (WHS), withers height measured with a tape (WHT), chest girth (CG), and cannon bone circumference (CBC). Based on these data, the bone index (BI) and body massiveness index (MI) were calculated. Statistical...
Analysis of ejaculate parameters and sperm morphology in roosters of initial laying strains
Ladislav Máchal, Tomá¹ Kopec, Zuzana Reèková, Radek Filipèík, Milan Veèeøa, Vojtìch Pe¹an, Eva Tùmová
Czech J. Anim. Sci., 2025, 70(2):72-81 | DOI: 10.17221/203/2024-CJAS
The aim of this study was to evaluate the influence of laying hybrid lines of domestic fowl (Gallus gallus f. domestica) and their age on ejaculate parameters in roosters. Parameters assessed included ejaculate volume, sperm concentration, total sperm count (TSC), percentage of normal spermatozoa, and sperm motility. Additionally, morphological defects were observed: defects of the acrosome, head, neck, tail, and immature spermatozoa. The study included 120 roosters of the Barred Plymouth Rock, Sussex Light, Rhode Island Red, and Rhode Island White laying lines. Ejaculate was repeatedly evaluated at the ages of 34, 43, and 52 weeks. The...
Broilers responses to dietary wormwood administration under Eimeria-challenged conditions
David Zapletal, Radka Dob¹íková, Martina Kos»uková, Vlastimil ©imek, Helena Støíbrná, Bøetislav Koudela
Czech J. Anim. Sci., 2025, 70(2):55-63 | DOI: 10.17221/188/2024-CJAS
Some herbs can be used as phytogenic additives as they naturally contain specific bioactive phytochemicals that are effective in controlling coccidiosis and some other poultry diseases due to their immunostimulatory effects and they can be also nutritious. The aim of the study was to evaluate the effect of Artemisia absinthium (WW) meal inclusion to a diet on the production performance, oocyst output and minerals content of meat in broilers infested by Eimeria oocysts. Ross 308 broilers were randomly assigned to four dietary groups. Broilers in the control group were fed basal diets without coccidiostat or WW. Broilers of the R...
Selected measurements of pork carcass geometry and association with slaughter value
Piotr Janiszewski, Dariuzs Lisiak, Karol Borzuta, Eugenia Grze¶kowiak, Piotr ¦lósarz
Czech J. Anim. Sci., 2025, 70(2):43-54 | DOI: 10.17221/161/2024-CJAS
The aim of the research was to use a vision technique to assess the relations between anatomical pork carcass traits, carcass geometry and slaughter value. The measurements in the experiment were performed on 128 selected pork carcasses. The 3D image of the carcass was assessed, especially its shape. Based on the carcass curvatures the correlation coefficients for these surfaces and slaughter value traits were calculated. The possibility of using the external dimensions and geometry of pork carcass for the accurate estimation of meatiness and the share of primal cuts using the technique of acquiring carcass images in a 3D configuration was confirmed....
Effect of dehulled lupin seeds in feed mixture on muscle protein quality of ducklings
Eva Straková, Lucie V¹etièková, Pavel Suchý, Martin Kutlva¹r
Czech J. Anim. Sci., 2025, 70(2):64-71 | DOI: 10.17221/9/2025-CJAS
The aim of the study was to prepare and test the effect of diets with 50% and 100% replacement of soybean meal with dehulled seeds of white lupin (Lupinus albus) variety Zulika. One hundred and eighty Cherry Valley duckling were divided to control group (C) and two experimental groups E50 (50% replacement of soybean meal with dehulled white lupin meal) and E100 (100% replacement of soybean meal with dehulled white lupin meal). There were evaluated the production parameters and the amino acid spectrum of breast and thigh muscles up to 42 days of age. The effect of diets containing lupin meal had no negative effect...
Association between polymorphism within rabbit IGF1 gene and slaughter weight in Termond White rabbits
Anna Migda³, Sylwia Ewa Pa³ka, Micha³ Kmiecik, Olga Jarnecka, Ewelina Semik-Gurgul, £ukasz Migda³
Czech J. Anim. Sci., 2025, 70(1):33-41 | DOI: 10.17221/59/2024-CJAS
Growth traits belong to the most important economic traits in livestock. One of the genes involved in vertebrate growth and development is insulin-like growth factor 1 (IGF1). Therefore, in our study we hypothesised that within the sequence of rabbit IGF1 gene it is possible to identify a polymorphism that may influence growth, carcass or meat traits in rabbits. We identified 6 polymorphisms (g.89259430T>C; g.89259338C>G; g.89259328T>C; 89210029A>G; 89210349C>G and g.89194199C>T) within introns of the IGF1 gene. One polymorphism, g.89194199C>T, was analysed using polymerase chain reaction high-resolution melting...
Somatic cells and bacteriological examination of milk samples of goats
Barbora Gancárová, Kristína Tvaro¾ková, Michal Uhrinèa», Lucia Maèuhová, Juliana Maèuhová, Vladimír Tanèin
Czech J. Anim. Sci., 2025, 70(1):26-32 | DOI: 10.17221/163/2024-CJAS
Mastitis, mainly caused by contagious bacteria, is an important disease in dairy goat production, especially subclinical mastitis. The aim of the research was to study the relationship between somatic cell count (SCC) and presence of mastitis pathogens in goat milk during the period 2022–2024. A total of 987 udder-half milk samples were obtained from 5 goat farms in Slovakia. The presence of pathogens was determined by bacterial cultivation and grown colonies were identified by MALDI-TOF MS at the species level. Fossomatic 7 was used to determine SCC. To evaluate the frequency distribution of the samples, the following groups of SCC were...
Validation of the evaluation of longevity by weighted analysis
Daniela Fulínová, Jiøí Bauer, Lubo¹ Vostrý
Czech J. Anim. Sci., 2025, 70(1):17-25 | DOI: 10.17221/157/2024-CJAS
We applied the Interbull validation methodology, a widely accepted method in animal breeding, to assess novel weighted and nonweighted repeatability models for the prediction of breeding values for longevity in Czech Holstein cattle. The population included in the estimation also contained 58 704 animals with genotypes. Performance records from 1 055 814 cows in the full dataset and from 831 995 cows in the trimmed dataset were used for evaluation. Both linear models included effects of herd-year-period, animal, permanent environment, and correction for milk production of individual cows in relation to herd average lactation curve, differing...
Mid-infrared milk screening as a phenotyping tool for feed efficiency in dairy cattle
Ludmila Zavadilová, Eva Ka¹ná, Zuzana Krupová, Alena Pechová, Petr Fleischer, Soòa ©losárková
Czech J. Anim. Sci., 2025, 70(1):1-16 | DOI: 10.17221/165/2024-CJAS
Feed efficiency (FE) is one of the most essential traits in dairy cattle, primarily due to the high cost of feed, which constitutes a significant portion of dairy herd expenses. Unfortunately, assessing FE in individual cows requires precise measurement of feed consumption, a labour-intensive and expensive process that is impractical for group-fed cows on production farms. Efforts have been made to predict FE or, more precisely, dry matter intake (DMI), using predictors such as a body weight (BW), milk yield (MY), and milk composition. Recently, Fourier transform mid-infrared (FT-MIR) spectroscopy has been proposed as a tool to enhance the accuracy...
A decision support system for herd health management for dairy farms
Jan Saro, Tomá¹ ©ubrt, Helena Bro¾ová, Robert Hlavatý, Jan Rydval, Jaromír Ducháèek, Ludìk Stádník
Czech J. Anim. Sci., 2024, 69(12):502-515 | DOI: 10.17221/178/2024-CJAS
Industrial dairy farms boast highly advanced health monitoring and disease diagnosis systems. But without easily accessible, user-friendly web platforms for real-time decision-making, most dairy farmers cannot proactively manage herd health management and optimize treatments based on disease prediction and prevention. To bridge this gap, we have developed a web application of a Decision support system (DSS) for dairy health management based on machine learning. The system architecture combines a Flask backend with a React frontend and scalable cloud data storage and includes preprocessing, data integration, predictive modelling, and cost analysis....
Effects of tryptophan supplementation on performance, intestinal morphology and protein abundance of tight junction protein and indoleamine 2, 3-dioxygenase in weaned pigs
Pei Mao, Naizhi Hu, Mengmeng Gao, Lingping Zhao, Chengsheng Song, Wenfeng Ma
Czech J. Anim. Sci., 2024, 69(12):493-501 | DOI: 10.17221/65/2024-CJAS
This study determined the effect of tryptophan (Trp) supplementation on the protein abundance of intestinal tight junction and indoleamine 2, 3-dioxygenase (IDO) in weaned pigs. Ninety-six White × Landrace × Duroc piglets (5.31 ± 0.54 kg) were selected in a growth trial and the experiment lasted for 30 days. The basal diet based on corn and soybean contained Trp at 2.4 g/kg. The dietary treatments consisted of a basal diet or a basal diet + 500 mg/kg Trp. On the 31st day, 12 pigs (1 pig per pen) were randomly selected and slaughtered in order to collect samples for subsequent analysis. Dietary supplementation with Trp improved the villus...
Effect of dehulled lupin seeds in feed mixture on muscle protein quality of broiler chickens
Eva Straková, Lucie V¹etièková, Pavel Suchý, Martin Kutlva¹r
Czech J. Anim. Sci., 2024, 69(12):484-492 | DOI: 10.17221/156/2024-CJAS
The aim of the study was to prepare and test the effect of diets with 50% and 100% replacement of soybean meal with dehulled seeds of white lupin (Lupinus albus) variety Zulika. Two hundred and forty ROSS 308 broiler chickens were divided into control group (C) and two experimental groups E50 (50% replacement of soybean meal with dehulled white lupin meal) and E100 (100% replacement of soybean meal with dehulled white lupin meal). The production parameters and the amino acid spectrum of breast and thigh muscles were evaluated up to 34 days of age. Final live weight of broiler chickens was not significantly affected...
Evaluation of weaning diets for sustainable indoor largemouth bass (Micropterus salmoides) larviculture
Jovanka Lukiæ, Norbert Vass, László Ardó, Jelena Stanivuk, Éva Lengyel-Kónya, Nata¹a Goliæ, Zsuzsanna Jakabné Sándor, Uro¹ Ljubobratoviæ
Czech J. Anim. Sci., 2024, 69(12):471-483 | DOI: 10.17221/129/2024-CJAS
This research evaluated the suitability of commercially available larval feeds, Otohime B2 (OB2), Aller Infa (AI), and Aqua Start (AS), and one Experimental Feed (EF), for the weaning of largemouth bass (Micropterus salmoides), LMB. Feeds were presented with various ω-3 fatty acid levels/bioavailability (high in OB2 and AI), fat percentage (high in OB2 and AS), free amino acid and short peptide (FAA + SP) levels (high in OB2), and various soluble protein (SPR) levels (high in AS and EF). Fish were co-fed Artemia plus OB2 from the 19th to 22nd day post-hatching (DPH), then Artemia in addition...
Pedigree analysis of the Latvian Warmblood horse heavy type population
Viktorija Nikonova, Daina Jonkus, Liga Paura
Czech J. Anim. Sci., 2024, 69(11):462-469 | DOI: 10.17221/147/2024-CJAS
A breeding program has been developed for the Latvian warmblood heavy type horses in Latvia, with the aim to preserve this type, because the number of these horses is small and the status of the local endangered breed has been granted. The aim of the study was to analyse the genetic structure, inbreeding and effective population size of the Latvian warmblood heavy type (LSB) horse population. Horses that were living at the time of the study on January 1, 2023 were used for the study. A total of 374 genetic resources live horses were analysed. Population structure analysis, inbreeding and effective population size were investigated. Calculations...
Dietary antioxidant supplementation improves the in vitro quality and antioxidant capacity of Colombian Creole stallion semen
Maria Claudia Puerta, Laura Henao-Salazar, Isabel Catalina Vélez, Sergio León, Banjamín Rojano, Giovanni Restrepo, Alexandra Úsuga
Czech J. Anim. Sci., 2024, 69(11):450-461 | DOI: 10.17221/98/2024-CJAS
Dietary antioxidant supplementation has become popular for managing male infertility because of its impact on the antioxidant capacity of semen. This study evaluates the effect of dietary antioxidant supplementation on the seminal characteristics of Colombian Creole horses. Semen from six Colombian Creole stallions was collected using an artificial vagina. The stallions were randomly allocated to a control group (CG) or a supplementation group (SG) that received a multi-antioxidant supplement. A repeated measures design, with a wash out period of two weeks between treatments, was used. Sperm motility and kinematics were assessed using a computer-assisted...
The effect of the dried Gracilaria spp. undergoing different drying methods on in vitro rumen fermentation
Farwa Shakeel, Yeni Widiawati, Sharly Asmairicen, Agung Purnomoadi, Agustin Herliatika, Yenny Nur Anggraeny, Setiasih Setiasih, Muhammad Rizwan, Ruslan Abdul Gopar, Antonius Antonius, Igit Atang Sunante, Idris Idris, Susi Riyanti, Winwin Widaringsih, Faizan Ahmad Shakir, Saad Irfan
Czech J. Anim. Sci., 2024, 69(11):441-449 | DOI: 10.17221/75/2024-CJAS
Seaweed has an important role in the mitigation of enteric methane (CH4) production by ruminant animals. The utilisation and its effectiveness in enteric CH4 reduction require a preservation process. The aim of the study was to investigate the effect of different drying processes on the effectiveness of seaweed Gracilaria spp. in reducing CH4 production assessed through an in vitro method. Three drying techniques, sun-drying, oven-drying, and freeze-drying, were applied to produce a dried product of Glacilaria spp. Rice straw basal diets combined with concentrate at the 70 : 30% were...
Quality and in vitro fertilising ability of cryostored Pinzgau bull insemination doses
Lucia Olexiková, Linda Dujíèková, Jaromír Va¹íèek, Andrej Balá¾i, Andrea Svoradová, Lenka Ku¾elová, Alexander Makarevich, Peter Ivaniè, Peter Chrenek
Czech J. Anim. Sci., 2024, 69(11):431-440 | DOI: 10.17221/142/2024-CJAS
Applicability of cryopreserved insemination doses is dependent on the quality of the semen after thawing. The aim of the study was to check the options of new markers for the assessment of sperm quality to predict their fertilisation ability. In five bulls of the Pinzgau breed, the sperm fertilising ability was determined using the in vitro fertilisation. Bulls were divided according to the achieved blastocyst rate: bulls with good fertilising ability (NOB – 38.6%, KAZ – 28.9%, GAL – 29.3%) and bulls with low fertilising ability (LOH – 19.4%, NUS – 22.1%). In addition to the sperm motility and morphology,...
A survey on rabbit meat perception and consumption in seven countries
Katalin Szendrõ, Antonella Dalle Zotte, Nikoletta Fülöp, Mónika Zita Nagy
Czech J. Anim. Sci., 2024, 69(10):419-429 | DOI: 10.17221/123/2024-CJAS
The aim of the study was to examine the rabbit consumption habits and opinions of people living in countries that regularly consume rabbit meat. Snowball sampling of data collection was used, a total of 1 860 error-free questionnaires were evaluated. Most of the completed questionnaires came from Hungary, Italy, Spain, China, Poland, France and Mexico. It is to note that 21.3% of respondents have not yet consumed rabbit meat: China (32.1%), Hungary (24.7%), Poland (22.4%), and France (4.8%). The main reasons for not consuming rabbit meat were emotional reasons (22.3%), it does not fit their dietary habits (15.7%). There are 28.7% of respondents...
Glutamine protects cow's ruminal epithelial cells from acid-induced injury in vitro
Yuanxiao Li, Yan Yu, Feiyan Zhao, Zihan Zhao, Mengying Dou, Zhijun Cao, Wang Li, Ke Ding, Cai Zhang
Czech J. Anim. Sci., 2024, 69(10):410-418 | DOI: 10.17221/170/2023-CJAS
This study was conducted to investigate the effects and mechanisms of glutamine (Gln) on the repair of acid-induced injury in dairy cow ruminal epithelial cells (RECs) in vitro. Dairy cow RECs were cultured in a medium with pH of 5.5 for 3 h and subsequently treated with various concentrations of Gln (4, 8, 12, 32 mmol/l) for 12 h. The messenger ribonucleic acid (mRNA) expression levels of occludin (OCLN), claudin 1 (CLDN1), toll-like receptor 2 (TLR2), toll-like receptor 4 (TLR4) and genes for inflammatory factors were measured using reverse transcription-quantitative real-time PCR (RT-qPCR)....
Comparative study: Efficacy of egg-yolk vs soy lecithin-based diluent in preservation of chilled bovine semen – Bacteriology and sperm quality
Michal Ïuraèka, Filip Benko, Miroslava Kaèániová, Eva Tvrdá
Czech J. Anim. Sci., 2024, 69(10):400-409 | DOI: 10.17221/138/2024-CJAS
This study compared the efficacy of the egg yolk (EY) and soy lecithin (SL) semen extender during 72-h storage at 4 °C in the presence/absence of streptomycin, penicillin, lincomycin, and spectinomycin. Bull semen samples (n = 12) were obtained and stored in either ANDROMED® (SL extender) or TRILADYL CSS® (EY extender). After 24, 48, and 72 h of storage at 4 °C, bacterial colonies (CFU – colony forming units) were grown and counted on tryptic soy and blood agar, and the bacterial isolates were identified using the MALDI-TOF MS Biotyper. Sperm motility, mitochondrial membrane potential (MMP), membrane...
Approach to creating an intelligent system for free-range livestock farming
Radka Malinova, Pencho Malinov, Evgeni Valchev, Todorka Glushkova
Czech J. Anim. Sci., 2024, 69(10):389-399 | DOI: 10.17221/90/2024-CJAS
The development of intelligent systems for the tracking of free-range livestock is a challenge to both information and communication technology (ICT) scientists and those in the animal sciences. Cyber-physical systems make it possible to track and control processes involving intelligent objects from the physical and virtual worlds. In the case of free-range grazing, it is necessary to manage processes in two domains ‒ that of the intelligent pasture management and that of the animals. Due to the differences in the conditions of different types of pastures – plain or high land and the characteristics of the cattle breeds, ready-made...