Czech J. Anim. Sci., 2026, 71(5):208-219 | DOI: 10.17221/6/2025-CJAS

Evaluation of the feeding value of microbially fermented cottonseed meal by in vitro methodOriginal Paper

Yutong Liu ORCID...1, Jiaming Luan1, Xin Feng1, Jinying Zhou1, Chunyin Geng1,2
1 Agricultural College, Yanbian University, Yanji, P.R. China
2 Ministry of Education, Engineering Research Center of North-East Cold Region Beef Cattle Science & Technology Innovation, Yanbian University, Yanji, P.R. China

In this study, different strains were used to ferment single/mixed cottonseed meal (cottonseed meal, corn flour, bran ratio of 7 : 2 : 1) substrates, and their feeding value was evaluated by an in vitro fermentation trial, to select suitable fermentation strains for cottonseed meal. For this purpose, two experiments (Exp.) were conducted. Exp. 1: Evaluation of the effect of fermentation of single cottonseed meal by different strains, which consisted of ten treatment groups: control (CON), Saccharomyces No. 1 (T1), Saccharomyces Fubon (T2), Saccharomyces Lallemand (T3), Lactobacillus (T4), Bacillus licheniformis (T5), Bacillus subtilis 10071 (T6), Bacillus subtilis 10089 (T7), Aspergillus niger (T8), and Monascus purpureus Went (T9). Exp. 2: Evaluation of the effect of fermentation of mixed cottonseed meal by different strains. The treatment groups were the same as in Exp. 1, numbered CON, M1, M2, M3, M4, M5, M6, M7, M8, and M9. The results showed that in both the single cottonseed meal group and the mixed cottonseed meal group free gossypol (FG) and methane (CH4) were significantly reduced and the gas production, total volatile fatty acids (TVFA), and in vitro digestibility of nutrients (P < 0.05) significantly increased, with Saccharomyces No. 1 showing the optimal effect. This study provides a theoretical basis for screening the suitable strains for fermenting cottonseed meal.

Keywords: detoxification efficiency; free gossypol; in vitro gas production; microorganisms; rumen fermentation

Received: January 14, 2025; Accepted: May 11, 2026; Prepublished online: May 28, 2026; Published: May 29, 2026  Show citation

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Liu Y, Luan J, Feng X, Zhou J, Geng C. Evaluation of the feeding value of microbially fermented cottonseed meal by in vitro method. Czech Journal of Animal Science. 2026;71(5):208-219. doi: 10.17221/6/2025-CJAS.
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