Czech J. Anim. Sci., 2025, 70(4):139-146 | DOI: 10.17221/159/2024-CJAS

Mesobiliverdin IXα-enriched microalgae feed improves gut health in weaned piglets under LPS challengeOriginal Paper

Tz-Shian Liao1, Pei-En Chang2, Yi-Ting Chen ORCID...1, Ching-Yi Chen ORCID...1, Cheng-Wei Tom Chang3, Jon YutakaTakemoto4, Yuan-Yu Lin ORCID...1
1 Department of Animal Science and Technology, National Taiwan University, Taipei City, Taiwan
2 Institute of Biotechnology, National Taiwan University, Taipei City, Taiwan
3 Department of Chemistry and Biochemistry, Utah State University, Logan, Utah, USA
4 Department of Biology, Utah State University, Logan, Utah, USA

This study aimed to evaluate the effect of mesobiliverdin IXα-enriched microalgae (MBV-SP) on growth, diarrhoea prevalence, intestinal morphology, antioxidant capacity, and inflammatory cytokines in lipopolysaccharide (LPS)-induced enteritis in weaned piglets. Seventy-two 28-day-old piglets were randomly divided into four groups: control (NC), LPS, tylosin, and MBV-SP, with saline/LPS (100 µg/kg) administered intraperitoneally on day 14. Results showed reduced mortality and diarrhoea rates in the tylosin + LPS and MBV-SP + LPS groups compared to the LPS group. Histological analysis revealed improved villus morphology and decreased crypt depth in the MBV-SP group. Catalase activity was notably higher in the MBV-SP group. Both tylosin and MBV-SP supplementation reduced pro-inflammatory cytokines, with MBV-SP exhibiting a more pronounced effect. In conclusion, MBV-SP mitigated diarrhoea, improved antioxidant capacity, and modulated inflammatory cytokines in weaned piglets with LPS-induced enteritis, highlighting its potential as a dietary supplement for the gut health in piglets.

Keywords: lipopolysaccharide; mesobiliverdin IXα; microalgae; weaned piglets

Received: October 4, 2024; Accepted: March 7, 2025; Prepublished online: April 28, 2025; Published: April 30, 2025  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Liao T, Chang P, Chen Y, Chen C, Chang CT, YutakaTakemoto J, Lin Y. Mesobiliverdin IXα-enriched microalgae feed improves gut health in weaned piglets under LPS challenge. Czech J. Anim. Sci. 2025;70(4):139-146. doi: 10.17221/159/2024-CJAS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Al-Sadi R, Boivin M, Ma T. Mechanism of cytokine modulation of epithelial tight junction barrier. Front Biosci (Landmark Ed). 2009 Jan;14(7):2765-78. Go to original source... Go to PubMed...
    2. Chang CT, Takemoto JY, Chang PE, AlFindee MN, Lin YY. Effects of mesobiliverdin IXalpha-enriched microalgae feed on gut health and microbiota of broilers. Front Vet Sci. 2021 Jan;7:586813. Go to original source... Go to PubMed...
    3. Drewe J, Beglinger C, Fricker G. Effect of ischemia on intestinal permeability of lipopolysaccharides. Eur J Clin Invest. 2001;31(2):138-44. Go to original source... Go to PubMed...
    4. Fan C, Han J, Liu X, Zhang F, Long Y, Xie Q. Modulation of hypoxia-inducible factor-1alpha/cyclo-oxygenase-2 pathway associated with attenuation of intestinal mucosa inflammatory damage by Acanthopanax senticosus polysaccharides in lipopolysaccharide-challenged piglets. Br J Nutr. 2019 Sep;122(6):666-75. Go to original source... Go to PubMed...
    5. Furbeyre H, van Milgen J, Mener T, Gloaguen M, Labussiere E. Effects of dietary supplementation with freshwater microalgae on growth performance, nutrient digestibility and gut health in weaned piglets. Animal. 2017 Feb;11(2):183-92. Go to original source... Go to PubMed...
    6. Furbeyre H, van Milgen J, Mener T, Gloaguen M, Labussiere E. Effects of oral supplementation with Spirulina and Chlorella on growth and digestive health in piglets around weaning. Animal. 2018 Nov;12(11):2264-73. Go to original source... Go to PubMed...
    7. Gresse R, Chaucheyras-Durand F, Fleury MA, van de Wiele T, Forano E, Blanquet-Diot S. Gut microbiota dysbiosis in postweaning piglets: Understanding the keys to health. Trends Microbiol. 2017 Oct;25(10):851-73. Go to original source... Go to PubMed...
    8. He L, Zhou X, Huang N, Li H, Cui Z, Tian J, Jiang Q, Liu S, Wu J, Li T, Yao K, Yin Y. Administration of alpha-ketoglutarate improves epithelial restitution under stress injury in early-weaning piglets. Oncotarget. 2017 Aug;8(54):91965-78. Go to original source... Go to PubMed...
    9. Ito T, Chen D, Chang CW, Kenmochi T, Saito T, Suzuki S, Takemoto JY. Mesobiliverdin IXα enhances rat pancreatic islet yield and function. Front Pharmacol. 2013 Mar 23;4:50. Go to original source... Go to PubMed...
    10. Lalles JP, Bosi P, Smidt H, Stokes CR. Nutritional management of gut health in pigs around weaning. Proc Nutr Soc. 2007 May;66(2):260-8. Go to original source... Go to PubMed...
    11. Li Y, Zhao X, Jiang X, Chen L, Hong L, Zhuo Y, Lin Y, Fang Z, Che L, Feng B, Xu S, Li J, Wu D. Effects of dietary supplementation with exogenous catalase on growth performance, oxidative stress, and hepatic apoptosis in weaned piglets challenged with lipopolysaccharide. J Anim Sci. 2020 Mar;98(3):skaa067. Go to original source... Go to PubMed...
    12. Liao TS, Chen CY, Lin CS, Chang CT, Takemoto JY, Lin YY. Mesobiliverdin IXα-enriched microalgae feed additive eliminates reliance on antibiotic tylosin to promote intestinal health of weaning piglets. J Anim Physiol Anim Nutr (Berl). 2023 Nov;107(6):1368-75. Go to original source... Go to PubMed...
    13. Merx MW, Weber C. Sepsis and the heart. Circulation. 2007 Aug;116(7):793-802. Go to original source... Go to PubMed...
    14. Miller BG, Whittemore CT, Stokes CR, Telemo E. The effect of delayed weaning on the development of oral tolerance to soya-bean protein in pigs. Br J Nutr. 1994 Apr;71(4):615-25. Go to original source... Go to PubMed...
    15. Pluske JR, Hampson DJ, Williams IH. Factors influencing the structure and function of the small intestine in the weaned pig: A review. Livest Prod Sci. 1997 Nov;51(1-3):215-36. Go to original source...
    16. Rhouma M, Fairbrother JM, Beaudry F, Letellier A. Post weaning diarrhea in pigs: Risk factors and non-colistin-based control strategies. Acta Vet Scand. 2017 May;59(1):31. Go to original source... Go to PubMed...
    17. Takemoto JY, Chang CWT, Chen D, Hinton G. Heme-derived bilins. Isr J Chem. 2019 May;59(5):378-86. Go to original source...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content