Czech J. Anim. Sci., 2025, 70(3):102-112 | DOI: 10.17221/72/2024-CJAS

LGALS3 promotes the proliferation of Liaoning Cashmere goat skin fibroblasts by regulating the expression of PIEZO1Original Paper

Mei Jin, Weiyu Fan, Jinxu Xu
Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery, College of Life Sciences, Liaoning Normal University, Dalian, P.R. China

Long non-coding RNA (lncRNA) plays a role in the reconstruction of hair follicles and in the formation and growth of cashmere fibres. One cashmere growth-associated lncRNA was successfully screened out at the early stage in the laboratory, which was named LncRNA MTC. In this study, the binding of galectin 3 (LGALS3) to LncRNA MTC was examined through RNA immunoprecipitation (RIP) in skin fibroblasts of Liaoning Cashmere goats. The pLenti-LGALS3-His-IRES-EGFP lentiviral overexpression vector was built, and the result of CCK8 indicated that the overexpression of LGALS3 significantly enhanced the viability of skin fibroblasts (P < 0.05). A total of 15 proteins interacting with LGALS3 were identified through co-immunoprecipitation (CO-IP) combined mass spectrometry, which were primarily rich in ECM-receptor interaction, focal adhesion, PI3K-AKT, and other signalling pathways. Moreover, PIEZO1 refers to a mechanically sensitive ion channel protein that is capable of regulating the cell proliferation. As revealed by qPCR results, overexpression of LGALS3 significantly upregulated the expression of PIEZO1, which was effectively interfered by siRNA. The result of CCK8 indicated that siPIEZO1 significantly inhibited cell proliferation, whereas overexpression of LGALS3 protected cells from the suppression of siPIEZO1 (P < 0.01). This study revealed that LGALS3 is capable of stimulating the proliferation of skin fibroblasts by regulating PIEZO1. The effect of LGALS3 on the proliferation of skin fibroblasts from the protein interaction was explored, and this study is expected to lay a certain scientific basis for the research on the hair follicle development mechanism of Liaoning Cashmere goats.

Keywords: co-immunoprecipitation; lentiviral overexpression vector; LncRNA MTC; RNA immunoprecipitation; siRNA

Received: May 16, 2024; Accepted: February 26, 2025; Prepublished online: March 24, 2025; Published: March 25, 2025  Show citation

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Jin M, Fan W, Xu J. LGALS3 promotes the proliferation of Liaoning Cashmere goat skin fibroblasts by regulating the expression of PIEZO1. Czech J. Anim. Sci. 2025;70(3):102-112. doi: 10.17221/72/2024-CJAS.
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