Czech J. Anim. Sci., 2023, 68(6):255-265 | DOI: 10.17221/178/2022-CJAS
Effects of dietary protease supplementation on behaviour, slaughter performance, meat quality and immune organ indices of broilersOriginal Paper
- 1 College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, P.R. China
- 2 Anhui Province Key Laboratory of Animal Nutrition Regulation and Health, Anhui, P.R. China
This study was conducted to investigate the effects of protease supplementation on the behaviour, slaughter performance, meat quality, and immune organ indices of broilers. A total of 240 one-day-old Arbor Acres broiler chickens were randomly allocated to four treatments, with six replicates per treatment and 10 broilers per replicate. Chickens were fed corn-soybean meal basal diets supplemented with 0, 4, 8, and 12 IU/g of protease. Compared with 0 IU/g of protease supplementation, 12 IU/g of protease increased the average and minimum time of feeding and drinking behaviour (P < 0.05); increased the average, maximum, and minimum time of preening behaviour (P < 0.05); and decreased feeding, walking, and stretching frequency (P < 0.05). pH24h of breast and thigh muscles had improved (P < 0.05). The shear force of the breast muscle and the cooking loss of the thigh muscle had decreased (P < 0.05). Compared with 0 IU/g of protease, 4 IU/g of protease increased the average and minimum time of lying behaviour (P < 0.05) and decreased stretching, scratching, and aggressive frequency (P < 0.05). The shear force of the thigh muscle and spleen index had enhanced (P < 0.05). Protease can be used as an additive in a broiler diet for production.
Keywords: enzyme; normal and abnormal behaviour; carcass traits; meat characteristics; immunity; poultry
Received: October 26, 2022; Accepted: April 28, 2023; Published: June 16, 2023 Show citation
ACS | AIP | APA | ASA | Harvard | Chicago | Chicago Notes | IEEE | ISO690 | MLA | NLM | Turabian | Vancouver |
References
- Angel CR, Saylor W, Vieira SL, Ward N. Effects of a monocomponent protease on performance and protein utilisation in 7- to 22-day-old broiler chickens. Poult Sci. 2011 Oct 1;90(10):2281-6.
Go to original source...
Go to PubMed...
- Appleby MC, Mench JA, Hughes BO. Poultry behaviour and welfare. London: CABI Pub; 2004.
Go to original source...
- Ashie INA, Sorensen TL, Nielsen PM. Effects of papain and a microbial enzyme on meat proteins and beef tenderness. J Food Sci. 2006 Jul 20;67(6):2138-42.
Go to original source...
- Chen XD, Ma QG, Tang MY, Ji C. Development of breast muscle and meat quality in Arbor Acres broilers, Jingxing 100 crossbred chickens and Beijing fatty chickens. Meat Sci. 2007 Mar 19;77(2):220-7.
Go to original source...
Go to PubMed...
- Choi YM, Kim BC. Muscle fiber characteristics, myofibrillar protein isoforms, and meat quality. Livest Sci. 2009 Jun 1;122(2-3):105-18.
Go to original source...
- Cowieson AJ, Abdollahi MR, Zaefarian F, Pappenberger G, Ravindran V. The effect of a mono-component exogenous protease and graded concentrations of ascorbic acid on the performance, nutrient digestibility and intestinal architecture of broiler chickens. Anim Feed Sci and Technol. 2018 Jan;235:128-37.
Go to original source...
- Dalolio FS, Vaz DP, Moreira J, Albino LFT, Valadares LR. Carcass characteristics of broilers fed enzyme complex. Biotechnol Anim Husbandry. 2016 Jan 22;31(2):153-62.
Go to original source...
- Doneva M, Miteva D, Dyankova S, Nacheva I, Metodieva P, Dimov K. Efficiency of plant proteases bromelain and papain on turkey meat tenderness. Biotechnol Anim Husbandry. 2015 Jul 14;31(3):407-13.
Go to original source...
- Duarte M, Gionbelli MP. 162 Fetal Programming and Meat Quality. J Anim Sci. 2021 Oct 8;99(Suppl 3):87.
Go to original source...
- Eklund B, Jensen P. Domestication effects on behavioural synchronisation and individual distances in chickens (Gallus gallus). Behav Processes. 2011 Feb 14;86(2):250-6.
Go to original source...
Go to PubMed...
- Feng Y, Yang XJ, Wang YB, Li WL, Liu Y, Yin RQ, Yao JH. Effects of immune stress on performance parameters, intestinal enzyme activity and mRNA expression of intestinal transporters in broiler chickens. Asian-Australas J Anim Sci. 2012 May 1;25(5):701-7.
Go to original source...
Go to PubMed...
- Fru-Nji F, Kluenter A, Fischer M, Pontoppidan K. A feed serine protease improves broiler performance and increases protein and energy digestibility. J Poult Sci. 2015 Aug 20;48(4):239-46.
Go to original source...
- Ghosh TK, Haldar S, Bedford MR, Muthusami N, Samanta I. Assessment of yeast cell wall as replacements for antibiotic growth promoters in broiler diets: Effects on performance, intestinal histo-morphology and humoral immune responses. J Anim Physiol Anim Nutr. 2011 Apr 30;96(2):275-84.
Go to original source...
Go to PubMed...
- Ikram A, Ambreen S, Tahseen A, Azhar A, Tariq K, Liaqat T, Zahid MBB, Rahim MA, Khalid W, Nasir N. Meat tenderisation through plant proteases - A mini review. Int J Biosci. 2021 Jan 20;18(1):102-12.
- Jabbar A, Tahir M, Khan RU, Ahmad N. Interactive effect of exogenous protease enzyme and dietary crude protein levels on growth and digestibility indices in broiler chickens during the starter phase. Trop Anim Health Prod. 2020 Nov 21;53: 23.
Go to original source...
Go to PubMed...
- Kamel NF, Naela, Ragaa M, El-Banna RA, Mohamed FF. Effects of a monocomponent protease on performance parameters and protein digestibility in broiler chickens. Agric Agric Sci Procedia. 2015 Dec;6:216-25.
Go to original source...
- Law FL, Zulkifli I, Soleimani AF, Liang JB, Awad EA. The effects of low-protein diets and protease supplementation on broiler chickens in a hot and humid tropical environment. Asian-Australas J Anim Sci. 2017 Dec 19;31(8):1291-300.
Go to original source...
Go to PubMed...
- Lee K, Hong YH, Lee S, Jang SI, Park M, Bautista DA, Ritter GD, Jeong W, Jeoung H, An D, Lillehoj EP, Lillehoj HS. Effects of anticoccidial and antibiotic growth promoter programs on broiler performance and immune status. Res Vet Sci. 2012 Jan 3;93(2):721-8.
Go to original source...
Go to PubMed...
- Lu P, Choi J, Yang C, Mogire M, Liu S, Lahaye L, Adewole D, Rodas-Gonzalez A, Yang C. Effects of antibiotic growth promoter and dietary protease on growth performance, apparent ileal digestibility, intestinal morphology, meat quality, and intestinal gene expression in broiler chickens: A comparison. J Anim Sci. 2020 Aug 10;98(9): skaa254.
Go to original source...
Go to PubMed...
- Mahmood T, Mirza MA, Nawaz H, Shahid M. Exogenous protease supplementation of poultry by-product meal-based diets for broilers: effects on growth, carcass characteristics and nutrient digestibility. J Anim Physiol Anim Nutr. 2017 Apr 28;102(1):e233-41.
Go to original source...
Go to PubMed...
- Matkawala F, Nighojkar S, Kumar A, Nighojkar A. Microbial alkaline serine proteases: Production, properties and applications. World J Microbiol Biotechnol. 2021 Mar 17;37(4): 12 p.
Go to original source...
Go to PubMed...
- Owsley WF, Orr DE, Tribble LF. Effects of age and diet on the development of the pancreas and the synthesis and secretion of pancreatic enzymes in the young pig. J Anim Sci. 1986 Aug;63(2):497-504.
Go to original source...
Go to PubMed...
- Peek HW, van der Klis JD, Vermeulen B, Landman WJM. Dietary protease can alleviate negative effects of a coccidiosis infection on production performance in broiler chickens. Anim Feed Sci Technol. 2009 Mar 30;150(1-2):151-9.
Go to original source...
- Riber AB, de Jong IC, van de Weerd HA, Steenfeldt S. Environmental enrichment for broiler breeders: An undeveloped field. Front Vet Sci. 2017 Jun 9;4: 6 p.
Go to original source...
Go to PubMed...
- Sarica S, Yurtseven S, Polat I. Supplementing diets for broilers that are low in crude protein and amino acids with protease. S Afr J Anim Sci. 2020 Aug 20;50(4):521-8.
Go to original source...
- Senaratna D, Samarakone TS, Gunawardane WWDA. Preference for different intensities of red light as affected by the age, temporal variation and behaviour of broiler birds. Trop Agric Res. 2015 Nov 23;25(2):146-57.
Go to original source...
- Sheikh SA, Wani MA, Rokade JJ, Tyagi PK, Biswas A, Tyagi PK, Mandal AB. Effect of feeding decorticated cottonseed meal with or without protease on blood biochemicals and immune status of broiler chicken. Indian J Poult Sci. 2016 Dec 3;50(1):28-32.
- Stefanello C, Vieira SL, Rios HV, Simoes CT, Sorbara JOB. Energy and nutrient utilisation of broilers fed soybean meal from two different Brazilian production areas with an exogenous protease. Anim Feed Sci Technol. 2016 Oct 23;221(Part B):267-73.
Go to original source...
- Xu X, Wang HL, Pan L, Ma XK, Tian QY, Xu YT, Long SF, Zhang ZH, Piao XS. Effects of coated proteases on the performance, nutrient retention, gut morphology and carcass traits of broilers fed corn or sorghum based diets supplemented with soybean meal. Anim Feed Sci Technol. 2017 Jan;223:119-27.
Go to original source...
- Yang JX, Maria TC, Zhou B, Xiao FL, Wang M, Mao YJ, Li Y. Quercetin improves immune function in Arbor Acre broilers through activation of NF-κB signaling pathway. Poult Sci. 2020 Jan 24;99(2):906-13.
Go to original source...
Go to PubMed...
- Zuo J, Ling B, Long L, Li T, Lahaye L, Yang C, Feng D. Effect of dietary supplementation with protease on growth performance, nutrient digestibility, intestinal morphology, digestive enzymes and gene expression of weaned piglets. Anim Nutr. 2015 Nov 6;1(4):276-82.
Go to original source...
Go to PubMed...
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.