Czech J. Anim. Sci., 2022, 67(7):266-274 | DOI: 10.17221/138/2021-CJAS

Effect of carboxymethyl chito-oligosaccharide on cysteine absorption in intestinal porcine epithelial cellsOriginal Paper

Huayu Huang1, Chen Zhang1, Luqiu Feng1, Naixiu Wang1, Kai Wang1, Zhenhui Song1, Ling Gan*,1,2,3
1 College of Veterinary Medicine, Southwest University, Chongqing, P.R. China
2 Key Laboratory of Pig Industry Sciences, Ministry of Agriculture and Rural Affairs, Chongqing, P.R. China
3 Chongqing Key Laboratory of Pig Industry Sciences, Chongqing, P.R. China

Oxidative stress is associated with retarded growth and the initiation and progression of diseases in pigs. The carboxymethylation of chito-oligosaccharide (COS) can improve its antioxidant activity for such issues. Herein, an orthogonal experiment L9 (34) test design was used to optimise the preparation conditions of carboxymethyl (CM)-COS. Fourier-transform infrared analysis evidenced the carboxymethylation of COS, and an in vitro study indicated that the hydroxyl radical scavenging ability of CM-COS is superior to that of COS. CM-COS can also better promote the absorption of cysteine and increase the expression of the amino acid transport system b0,+ in intestinal porcine epithelial cells. The results suggested that CM-COS can effectively resist oxidative damage by promoting cysteine absorption mediated by transport system b0,+, which provides important information regarding the antioxidative damage application of COS and CM-COS in the pig farming industry.

Keywords: carboxymethyl modification; antioxidant activity; amino acid transport system; intestinal health

Published: July 30, 2022  Show citation

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Huang H, Zhang C, Feng L, Wang N, Wang K, Song Z, Gan L. Effect of carboxymethyl chito-oligosaccharide on cysteine absorption in intestinal porcine epithelial cells. Czech J. Anim. Sci. 2022;67(7):266-274. doi: 10.17221/138/2021-CJAS.
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