Czech J. Anim. Sci., 2021, 66(8):331-338 | DOI: 10.17221/57/2021-CJAS

Efficacy of poly (lactic-co-glycolic acid) microparticles as a gonadotropin-releasing hormone analogue delivery system to stimulate ovulation of peled Coregonus peledOriginal Paper

Jindřiška Knowles*,1, Jakub Vysloužil2, Jan Muselík2, Vlastimil Stejskal1, Jan Kouřil1, Peter Podhorec1
1 Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Institute of Aquaculture and Protection of Waters, University of South Bohemia in České Budějovice, České Budějovice, Czech Republic
2 Department of Pharmaceutical Technology, Faculty of Pharmacy, Masaryk University, Brno, Czech Republic

The aim of the study was to determine the efficacy of poly (lactic-co-glycolic acid) microparticles as a carrier of gonadotropin-releasing hormone analogues (GnRHa) for induction of ovulation in peled Coregonus peled. Female peled were injected intraperitoneally with 1) saline solution only (control), 2) mammalian GnRHa at 25 µg/kg body weight, 3) GnRHa in 753-type microparticles at 50 µg/kg, or 4) GnRHa in 653-type microparticles at 50 µg/kg. Blood plasma samples were taken on days 0, 4, 8, and 12 post-injection. All hormone treatments induced synchronous ovulation and higher cumulative ovulation compared to controls. Hormone treatments did not affect relative fecundity or the percentage of eyed eggs. Testosterone level decreased toward the onset of ovulation. On day eight of the trial, the testosterone level was significantly lower in hormone-treated groups compared to the control group. The level of 17β-oestradiol showed a decreasing trend post-injection, with the lowest observed level on day eight. Our results demonstrate that ovulation can be induced in the peled by the sustained - release of GnRHa in poly (lactic-co-glycolic acid) microparticles, but the treatment does not improve reproductive performance.

Keywords: induction of ovulation; GnRHa; salmonids; sustained drug release; steroid feedback

Published: July 28, 2021  Show citation

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Knowles J, Vysloužil J, Muselík J, Stejskal V, Kouřil J, Podhorec P. Efficacy of poly (lactic-co-glycolic acid) microparticles as a gonadotropin-releasing hormone analogue delivery system to stimulate ovulation of peled Coregonus peled. Czech J. Anim. Sci. 2021;66(8):331-338. doi: 10.17221/57/2021-CJAS.
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References

  1. Agulleiro MJ, Anguis V, Canavate JP, Martinez-Rodriguez G, Mylonas CC, Cerda J. Induction of spawning of captivereared Senegal sole (Solea senegalensis) using different administration methods for gonadotropin-releasing hormone agonist. Aquaculture. 2006 Jun 30;257(1-4):511-24. Go to original source...
  2. Anderson K, Pankhurst N, King H, Elizur A. Effects of GnRHa treatment during vitellogenesis on the reproductive physiology of thermally challenged female Atlantic salmon (Salmo salar). PeerJ. 2017 Oct 20;5: 24 p. Go to original source... Go to PubMed...
  3. Arabaci M, Diler I, Sari M. Induction and synchronisation of ovulation in rainbow trout, Oncorhynchus mykiss, by administration of emulsified buserelin (GnRHa) and its effects on egg quality. Aquaculture. 2004 Aug 2;237 (1-4):475-84. Go to original source...
  4. Billard R, Reinaud P, Hollebecq M, Breton B. Advancement and synchronisation of spawning in Salmo gairdneri and S. trutta following administration of LRH-A combined or not with pimozide. Aquaculture. 1984 Dec 15;43(1-3):57-66. Go to original source...
  5. Bobe J, Labbe C. Egg and sperm quality in fish. Gen Comp Endocrinol. 2010 Feb 1;165(3):535-48. Go to original source... Go to PubMed...
  6. Bonnet E, Fostier A, Bobe J. Microarray-based analysis of fish egg quality after natural or controlled ovulation. BMC Genom. 2007 Dec;8(1):1-17. Go to original source... Go to PubMed...
  7. Breton B, Weil C, Sambroni E, Zohar Y. Effects of acute versus sustained administration of GnRHa on GtH release and ovulation in the rainbow trout, Oncorhynchus mykiss. Aquaculture. 1990 Dec 15;91(3-4):373-83. Go to original source...
  8. da Silva MTS, Pinto JC. Influence of encapsulated aroma compounds on the formation and morphology of gelatin microparticles. Macromol Symp. 2019 Feb 15;383(1): 11 p. Go to original source...
  9. Dabrowski K, Ciereszko A, Ramseyer L, Culver D, Kestemont P. Effects of hormonal treatment on induced spermiation and ovulation in the yellow perch (Perca flavescens). Aquaculture. 1994 Feb 15;120(1-2):171-80. Go to original source...
  10. Dickhoff WW, Yan L, Plisetskaya EM, Sullivan CV, Swanson P, Hara A, Bernard MG. Relationship between metabolic and reproductive hormones in salmonid fish. Fish Physiol Biochem. 1989 Jun;7(1):147-55. Go to original source... Go to PubMed...
  11. Duvvuri S, Janoria KG, Mitra AK. Development of a novel formulation containing poly (d,l-lactide-co-glycolide) microspheres dispersed in PLGA-PEG-PLGA gel for sustained delivery of ganciclovir. J Control Release. 2005 Nov 28;108(2-3):282-93. Go to original source... Go to PubMed...
  12. Garber AF, Fordham SE, Symonds JE, Trippel EA, Berlinsky DL. Hormonal induction of ovulation and spermiation in Atlantic cod (Gadus morhua). Aquaculture. 2009 Nov 1;296(1-2):179-83. Go to original source...
  13. Gordeeva NV, Karmanova OG, Shitova MV. Genetic and morphoecological characteristics of peled Coregonus peled acclimatised in lakes of Tuva Republic. J Ichthyol. 2008 Sep 30;48(8):573-82. Go to original source...
  14. Goren A. Field trials demonstrate the efficacy and commercial benefit of a GnRHa implant to control ovulation and spermiation in salmonids. In: Goetz FW, Thomas P, editors. Proceeding of the 5th International Symposium on the Reproductive Physiology of Fish; 1995 Jul 2-8; University of Texas at Austin. Austin, TX: Fish Symposium 95; 1995. p. 99-101.
  15. Hochman L. Chov sihu [Culture of coregonid fishes]. Vodnany, Czech Republic: RIFCH Vodnany; 1987. 16 p. Czech.
  16. Jerez S, Fakriadis I, Papadaki M, Martin M, Cejas JR, Mylonas CC. Spawning induction of first-generation (F1) greater amberjack Seriola dumerili in the Canary Islands, Spain using GnRHa delivery systems. Fishes. 2018 Sep;3(3): 22 p. Go to original source...
  17. Kallert DM. Der Einfluss exogener und endogener Parameter auf den Erbrutungserfolg bei Salmoniden [The influence of exogenous and endogenous parameters on the fertilization-success of salmonids]. Bayreuth, Germany: Bezirk Oberfranken; 2009. 144 p. German.
  18. Lin Z, Gao W, Hu H, Ma K, He B, Dai W, Wang X, Wang J, Zhang X, Zhang Q. Novel thermo-sensitive hydrogel system with paclitaxel nanocrystals: High drug-loading, sustained drug release and extended local retention guaranteeing better efficacy and lower toxicity. J Control Release. 2014 Jan 28;174:161-70. Go to original source... Go to PubMed...
  19. Makadia HK, Siegel SJ. Poly lactic-co-glycolic acid (PLGA) as biodegradable controlled drug delivery carrier. Polymers. 2011 Sep;3(3):1377-97. Go to original source... Go to PubMed...
  20. Matejkova J, Podhorec P. Sustained drug delivery system in fish and the potential for use of PLGA microparticles: A review. Vet Med-Czech. 2019 Jul 25;64(7):287-93. Go to original source...
  21. Matousek J, Gebauer T, Stejskal V. Effect of weaning initiation time and feed pellet size on peled Coregonus peled (Gmelin 1789) larviculture. Aquac Res. 2020 May; 51(5):2150-4. Go to original source...
  22. Mikolajczyk T, Kuzminski H, Dobosz S, Goryczko K, Enright W. The effects of GonazonTM, a commercially available GnRH analogue, on induction of ovulation and egg quality in cultured European whitefish (Coregonus lavaretus L.). Adv Limnol. 2007 Jan;60:187-94.
  23. Mikolajczyk T, Sokolowska-Mikolajczyk M, Szczerbik P, Duc M, Goryczko K, Dobosz S, Glogowski J, Epler P, Enright WJ. The effects of the GnRH agonist, azagly-nafarelin (GonazonTM), on ovulation and egg viability in the European grayling (Thymallus thymallus L.). Aquaculture. 2008 Sep 1;281(1-4):126-30. Go to original source...
  24. Mylonas CC, Hinshaw JM, Sullivan CV. GnRHa-induced ovulation of brown trout (Salmo trutta) and its effects on egg quality. Aquaculture. 1992 Sep 15;106(3-4):379-92. Go to original source...
  25. Noori A, Amiri BM, Mirvaghefi A, Baker DW. LHRHa induced ovulation of the endangered Caspian brown trout (Salmo trutta caspius) and its effect on egg quality and two sex steroids: Testosterone and 17α hydroxyprogestrone. Aquac Res. 2010 May;41(6):871-7. Go to original source...
  26. Norberg B, Bjornsson BT, Brown CL, Wichardt UP, Deftos LJ, Haux C. Changes in plasma vitellogenin, sex steroids, calcitonin, and thyroid hormones related to sexual maturation in female brown trout (Salmo trutta). Gen Comp Endocrinol. 1989 Aug 1;75(2):316-26. Go to original source... Go to PubMed...
  27. Olito C, Loopstra D, Hansen P. Acceleration of sexual maturation in chinook salmon broodstock using luteinising hormone-releasing hormone analog. N Am J Aquac. 2001 Jul 1;63(3):208-14. Go to original source...
  28. Park W, Lee CH, Lee CS, Kim DJ, Kim JH, Tamaru CS, Sohn YC. Effects of a gonadotropin-releasing hormone analog combined with pimozide on plasma sex steroid hormones, ovulation and egg quality in freshwater-exposed female chum salmon (Oncorhynchus keta). Aquaculture. 2007 Oct 3;271(1-4):488-97. Go to original source...
  29. Savini D, Occhipinti-Ambrogi A, Marchini A, Tricarico E, Gherardi F, Olenin S, Gollasch S. The top 27 animal alien species introduced into Europe for aquaculture and related activities. J Appl Ichthyol. 2010 Aug;26(Suppl. 2):1-7. Go to original source...
  30. Schwach G, Oudry N, Delhomme S, Luck M, Lindner H, Gurny R. Biodegradable microparticles for sustained release of a new GnRH antagonist - part I: Screening commercial PLGA and formulation technologies. Eur J Pharm Biopharm. 2003 Nov 1;56(3):327-36. Go to original source... Go to PubMed...
  31. Shah NK, Wang Z, Gupta SK, Le Campion A, Meenach SA. Sustained release of a model water-soluble compound via dry powder aerosolizable acetalated dextran microparticles. Pharm Dev Technol. 2019 Oct 21;24(9):1133-43. Go to original source... Go to PubMed...
  32. Siepmann J, Siepmann F. Microparticles used as drug delivery systems. In: Richtering W, editor. Smart colloidal materials. Berlin: Springer-Verlag Berlin Heidelberg; 2006. p. 15-21. Go to original source...
  33. Stejskal V, Matousek J, Prokesova M, Podhorec P, Sebesta R, Drozd B. Combined effect of weaning time and co-feeding duration on growth and survival of peled Coregonus peled (Gmelin) larvae. Aquac Nutr. 2018 Feb;24(1):434-41. Go to original source...
  34. Strobel SA, Hudnall K, Arbaugh B, Cunniffe JC, Scher HB, Jeoh T. Stability of fish oil in calcium alginate microcapsules cross-linked by in situ internal gelation during spray drying. Food Bioproc Tech. 2020 Feb;13(2):275-87. Go to original source...
  35. Svinger VW, Policar T, Steinbach C, Polakova S, Jankovych A, Kouril J. Synchronization of ovulation in brook char (Salvelinus fontinalis, Mitchill 1814) using emulsified dArg 6Pro9NEt sGnRHa. Aquac Int. 2013 Aug;21(4):783-99. Go to original source...
  36. Szmyt M, Piech P, Panasiewicz G, Niewiadomski P, PietrzakFiecko R. Gonadal steroid fluctuations and reproduction results of European grayling (Thymallus thymallus L.) from broodstock farmed in a recirculation aquaculture system. Anim Reprod Sci. 2021 Jan 1;224: 9 p. Go to original source... Go to PubMed...
  37. Taranger GL, Haux C, Stefansson SO, Bjornsson BT, Walther BT, Hansen T. Abrupt changes in photoperiod affect age at maturity, timing of ovulation and plasma testosterone and oestradiol-17β profiles in Atlantic salmon, Salmo salar. Aquaculture. 1998 Mar 1;162(1-2):85-98. Go to original source...
  38. Vazirzadeh A, Hajimoradloo A, Esmaeili HR, Akhlaghi M. Effects of emulsified versus saline administration of GnRHa on induction of ovulation in rainbow trout, Oncorhynchus mykiss. Aquaculture. 2008 Aug 1;280(1-4):267-9. Go to original source...
  39. Vyslouzil J, Dvorackova K, Kejdusova M, Rabiskova M. Priprava lecivych mikrocastic metodou odparovani rozpoustedla [Preparation of Medicinal Microparticles by Solvent Evaporation Method]. Chem Listy. 2013 Jan; 107:16-23. Czech.
  40. Zarski D, Fontaine P, Roche J, Alix M, Blecha M, Broquard C, Krol J, Milla S. Time of response to hormonal treatment but not the type of a spawning agent affects the reproductive effectiveness in domesticated pikeperch, Sander lucioperca. Aquaculture. 2019 Mar 30;503:527-36. Go to original source...
  41. Zohar Y, Mylonas CC. Endocrine manipulations of spawning in cultured fish: From hormones to genes. In: Lee CS, Donaldson EM, editors. Reproductive biotechnology in Finfish aquaculture. 1st ed. Amsterdam: Elsevier; 2001. p. 99-136. Go to original source...

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