Czech J. Anim. Sci., 2018, 63(11):429-434 | DOI: 10.17221/122/2017-CJAS

Effects of chilled storage and pH of activating solution on different motility parameters in burbot (Lota lota) spermOriginal Paper

Zoltán Bokor1, Balázs Csorbai1, Levente Várkonyi1, Zsolt Szári2, Ferenc Fodor2, István Ittzés3, Daniel Żarski4, Béla Urbányi1, Gergely Bernáth*,1
1 Department of Aquaculture, Szent István University, Gödöllő, Hungary
2 Balaton Fish Management Non-Profit Ltd, Siófok, Hungary
3 Self-entrepreneur, H-2425 Nagykarácsony, Hungary
4 Department of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Olsztyn, Poland

The effects of a simple saline solution prepared using two different pH (4.4 and 8.5) on sperm motility in burbot were investigated. Results were recorded during a 96-hour chilled storage (4°C) in 24-hour intervals. Measurements were focused on the detailed characteristics of motility using 12 parameters obtained from the Computer-assisted Sperm Analysis (CASA). Significantly higher progressive motility (pMOT), distance average path (DAP), distance curved line, distance straight line (DSL), average path velocity (VAP), curvilinear velocity, straight line velocity, and beat cross frequency (BCF) were observed with the activating solution buffered at pH 8.5 in comparison with pH 4.4. Already after 24 h a significant reduction was measured in pMOT (0 h: 49 ± 24%, 24 h: 12 ± 7%). Similar decreasing tendency was recorded only after 72 h in DAP (0 h: 26 ± 4 µm/s, 72 h: 19 ± 9 µm/s), DSL (0 h: 21 ± 5 µm/s, 72 h: 17 ± 8 µm/s), VAP (0 h: 59 ± 9 µm/s, 72 h: 43 ± 21 µm/s), and BCF (0 h: 28 ± 2 Hz, 72 h: 18 ± 10 Hz). The response of different investigated CASA parameters to different treatments varied in our experiments. According to our studies, numerous burbot sperm motility parameters are sensitive to chilled storage and to low pH of the activating solution. Our results could support the effective sperm quality assessment and successful artificial propagation process in burbot.

Keywords: short-term storage; activation; Lota lota; sperm analysis; CASA parameters

Published: November 30, 2018  Show citation

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Bokor Z, Csorbai B, Várkonyi L, Szári Z, Fodor F, Ittzés I, et al.. Effects of chilled storage and pH of activating solution on different motility parameters in burbot (Lota lota) sperm. Czech J. Anim. Sci. 2018;63(11):429-434. doi: 10.17221/122/2017-CJAS.
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