Czech J. Anim. Sci., 2016, 61(4):194-202 | DOI: 10.17221/8851-CJAS

Effects of retinoic acid on the synthesis of selenoprotein and the antioxidative indices of bovine mammary epithelial cells in vitroOriginal Paper

L. Jin, S. Yan, B. Shi, R. Sheng, H. Shi, Y. Zhao, J. Li
College of Animal Science, Inner Mongolia Agricultural University, Hohhot, P.R. China

The present study was conducted to examine the effects of retinoic acid (RA) on the synthesis of selenoprotein and the antioxidative indices of bovine mammary epithelial cells (BMEC) in vitro and to explore the antioxidative mechanisms of RA in the BMEC. The subconfluenced BMEC were divided into six treatments with six replicates per treatment and cultured in a Dulbecco's Modified Eagle's Medium/F12 media (10% fetal bovine serum, 5 µg/ml ovine prolactin, 10 ng/ml epidermal growth factor, 1 g/ml hydrocortisone, 0.5% insulin-transferrin-selenium) containing different levels of RA (0 (control), 0.05, 0.1, 0.2, 1 or 2 mg/ml) for 24 h. Addition of RA promoted the proliferation of BMEC, increased the activities of catalase, superoxide dismutase, total antioxidant capacity, glutathione peroxidase (GPX), thioredoxin reductase (TRXR), and the content of selenoprotein P (SELP) in a dose-dependent manner (P < 0.05). The optimal RA dose was 1 μg/ml. However, positive effect of RA tended to be suppressed when RA was increased to 2 μg/ml. The expressions of mRNA and protein of GPX in BMEC were up-regulated by RA in a quadratic dose-response relationship (P < 0.01), and the addition of 1 μg/ml RA showed the best effect. The mRNA expressions of TRXR1 and SELP as well as the protein expression of TRXR1 were higher at 1-2 μg/ml RA. These results suggested that RA promoted antioxidant function of BMEC by regulating the synthesis of selenoprotein including GPX, TRXR, and SELP in vitro.

Keywords: vitamin A; antioxidant function; dairy cows

Published: April 30, 2016  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Jin L, Yan S, Shi B, Sheng R, Shi H, Zhao Y, Li J. Effects of retinoic acid on the synthesis of selenoprotein and the antioxidative indices of bovine mammary epithelial cells in vitro. Czech J. Anim. Sci. 2016;61(4):194-202. doi: 10.17221/8851-CJAS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Arner E.S.J., Holmgren A. (2000): Physiological functions of thioredoxin and thioredoxin reductase. European Journal of Biochemistry, 267, 6102-6109. Go to original source... Go to PubMed...
    2. Balmer J.E., Blomhoff R. (2002): Gene expression regulation by retinoic acid. Journal of Lipid Research, 43, 1773-1808. Go to original source... Go to PubMed...
    3. Basirico L., Morera P., Dipasquale D., Troscher A., Serra A., Mele M., Bernabucci U. (2015): Conjugated linoleic acid isomers strongly improve the redox status of bovine mammary epithelial cells (BME-UV1). Journal of Dairy Science, 98, 7071-7082. Go to original source... Go to PubMed...
    4. Bruzelius K., Hoac T., Sundler R., Onning G., Akesson B. (2007): Occurrence of selenoprotein enzyme activities and mRNA in bovine mammary tissue. Journal of Dairy Science, 90, 918-927. Go to original source... Go to PubMed...
    5. Bruzelius K., Purup S., James P., Onning G., Akesson B. (2008): Biosynthesis of selenoproteins in cultured bovine mammary cells. Journal of Trace Elements in Medicine and Biology, 22, 224-233. Go to original source... Go to PubMed...
    6. Bruzelius K., Sundler R., Pagmantidis V., Akesson B. (2010): Regulation of selenoprotein mRNA expression by hormones and retinoic acid in bovine mammary cells. Journal of Trace Elements in Medicine and Biology, 24, 251-256. Go to original source... Go to PubMed...
    7. Burk R.F., Hill K.E. (2005): Selenoprotein P: an extracellular protein with unique physical characteristics and a role in selenium homeostasis. Annual Review of Nutrition, 25, 215-235. Go to original source... Go to PubMed...
    8. Chen C.C., Wang J.K. (1999): p38 but not p44/42 mitogen-activated protein kinase is required for nitric oxide synthase induction mediated by lipopolysaccharide in RAW 264.7 macrophages. Molecular Pharmacology, 55, 481-488. Go to original source...
    9. Chu F.F., Esworthy R.S., Lee L., Wilczynski S. (1999): Retinoic acid induces GPX2 gene expression in MCF-7 human breast cancer cells. The Journal of Nutrition, 129, 1846-1854. Go to original source... Go to PubMed...
    10. David O.S., Jason S.C., Joshua D.N., Adam J.F. (2012): The potential of nitric oxide releasing therapies as antimicrobial agents. Landes Bioscience, 33, 271-279. Go to original source... Go to PubMed...
    11. De Marchi F.E., Palin M.F., dos Santos G.T., Lima L.S., Benchaar C., Petit H.V. (2015): Flax meal supplementation on the activity of antioxidant enzymes and the expression of oxidative stress- and lipogenic-related genes in dairy cows infused with sunflower oil in the abomasum. Animal Feed Science and Technology, 199, 41-50. Go to original source...
    12. Ferret P.J., Soum E., Negre O., Wollman E.E., Fradelizi D. (2000): Protective effect of thioredoxin upon NOmediated cell injury in THP1 monocytic human cells. Biochemical Journal, 346, 759-765. Go to original source...
    13. Hill K.E., Xia Y., Akesson B., Boeglin M.E., Burk R.F. (1996): Selenoprotein P concentration in plasma is an index of selenium status in selenium-deficient and seleniumsupplemented Chinese subjects. The Journal of Nutrition, 126, 138-145. Go to original source... Go to PubMed...
    14. Hill K.E., McCollum G.M., Burk R.F. (1997): Determination of thioredoxin reductase activity in rat liver supernatant. Analytical Biochemistry, 253, 123-125. Go to original source... Go to PubMed...
    15. Jin L., Yan S.M., Shi B.L., Bao H.Y., Gong J., Guo X.Y., Li J.L. (2014): Effect of vitamin A on milk performance, antioxidant and immune function of dairy cows. Animal Feed Science and Technology, 192, 15-23. Go to original source...
    16. Kim S.H., Johnson V.J., Sharma R.P. (2004): Selenium attenuates lipopolysaccharide-induced oxidative stress responses through modulation of p38 MAPK and NFkappaB signaling pathways. Experimental Biology and Medicine, 229, 203-213. Go to original source... Go to PubMed...
    17. Kohrle J. (2005): Selenium and the control of thyroid hormone metabolism. Thyroid, 15, 841-853. Go to original source... Go to PubMed...
    18. Kurosawa T., Nakamura H., Yamaura E., Fujino H., Matsuzawa Y., Kawashima T., Murayama T. (2009): Cytotoxicity induced by inhibition of thioredoxin reductases via multiple signaling pathways: role of cytosolic phospholipase A2α-dependent and -independent release of arachidonic acid. Journal of Cellular Physiology, 219, 606-616. Go to original source... Go to PubMed...
    19. Lima L.S., Palin M.F., Santos G.T., Benchaar C., Petit H.V. (2015): Effects of supplementation of flax meal and flax oil on mammary gene expression and activity of antioxidant enzymes in mammary tissue, plasma and erythrocytes of dairy cows. Livestock Science, 176, 196-204. Go to original source...
    20. Liu L., Lin Y., Liu L., Bian Y., Zhang L., Gao X., Li Q. (2015): 14-3-3γ regulates lipopolysaccharide-induced inflammatory responses and lactation in dairy cow mammary epithelial cells by inhibiting NF-κB and MAPKs and up-regulating mTOR signaling. International Journal of Molecular Sciences, 16, 16622-16641. Go to original source... Go to PubMed...
    21. Livak K.J., Schmittgen T.D. (2001): Analysis of relative gene expression data using real time quantitative PCR and the 2-ΔΔCt method. Methods, 25, 402-408. Go to original source... Go to PubMed...
    22. Ma X.M., Yang Z.B., Yang W.R., Song M.L. (2005): Effect of different vitamin A levels in diets on antioxidant ability of beef cattle. Chinese Journal of Animal Nutrition, 17, 31-35. (in Chinese)
    23. Machino T., Hashimoto S., Maruoka S., Gon Y., Hayashi S., Mizumura K., Nishitoh H., Ichijo H., Horie T. (2003): Apoptosis signal-regulating kinase 1-mediated signaling pathway regulates hydrogen peroxide-induced apoptosis in human pulmonary vascular endothelial cells. Critical Care Medicine, 31, 2776-2781. Go to original source... Go to PubMed...
    24. Menth M., Schmutzler C., Mentrup B., Hoang-Vu C., Takahashi K., Honjoh T., Kohrle J. (2005): Selenoprotein expression in Hürthle cell carcinomas and in the human Hürthle cell carcinoma line XTC. UC1. Thyroid, 15, 405-416. Go to original source... Go to PubMed...
    25. Miranda R., Blanco A., Negro C. (2009): Accumulation of dissolved and colloidal material in papermaking - Application to simulation. Chemical Engineering Journal, 148, 385-393. Go to original source...
    26. Palacios A., Piergiacomi V., Catala A. (1996): Vitamin A supplementation inhibits chemiluminescence and lipid peroxidation in isolated rat liver microsomes and mitochondria. Molecular and Cellular Biology, 154, 77-82. Go to original source... Go to PubMed...
    27. Papp L.V., Lu J., Holmgren A., Khanna K.K. (2007): From selenium to selenoproteins: synthesis, identity, and their role in human health. Antioxidants and Redox Signalling, 9, 775-806. Go to original source... Go to PubMed...
    28. Qiao L. (2008): Study on the rule of degradation of vitamin A in rumen and evaluation of effects of ruminal protected vitamin A on dairy cows. Ph.D. Thesis. Hohhot, China: Inner Mongolia Agricultural University. (in Chinese)
    29. Ramauge M., Pallud S., Esfandiari A., Gavaret J., Lennon A., Pierre M., Courtin F. (1996): Evidence that type III iodothyronine deiodinase in rat astrocyte is a selenoprotein. Endocrinology, 137, 3021-3025. Go to original source... Go to PubMed...
    30. Rock C., Moos P.J. (2010): Selenoprotein P protects cells from lipid hydroperoxides generated by 15-LOX-1. Prostaglandins, Leukotrienes and Essential Fatty Acids, 83, 203-210. Go to original source... Go to PubMed...
    31. Schmidt H.H.H.W., Walter U. (1994): NO at work. Cell, 78, 919-925. Go to original source... Go to PubMed...
    32. Smith K.L., Hogan J.S., Weiss W.P. (1997): Dietary vitamin E and selenium affect mastitis and milk quality. Journal of Animal Science, 75, 1659-1665. Go to original source... Go to PubMed...
    33. Sordillo L.M., Aitken S.L. (2009): Impact of oxidative stress on the health and immune function of dairy cattle. Veterinary Immunology and Immunopathology, 128, 104-109. Go to original source... Go to PubMed...
    34. Sordillo L.M., Boyle N.O., Gandy J.C., Corl C.M., Hamilton E. (2007): Shifts in thioredoxin reductase activity and oxidant status in mononuclear cells obtained from transition dairy cattle. Journal of Dairy Science, 90, 1186-1192. Go to original source... Go to PubMed...
    35. Tobiume K., Saitoh M., Ichijo H. (2002): Activation of apoptosis signal-regulating kinase 1 by the stress-induced activating phosphorylation of preformed oligomer. Journal of Cellular Physiology, 191, 95-104. Go to original source... Go to PubMed...
    36. Wang Y.K., Hong Y.G., Wei M., Wu Y., Huang Z.Q., Chen R.Z., Chen H.Z. (2010): Curculigoside attenuates human umbilical vein endothelial cell injury induced by H 2O2. Journal of Ethnopharmacology, 132, 233-239. Go to original source... Go to PubMed...
    37. Wellnitz O., Kerr D.E. (2004): Cryopreserved bovine mammary cells to model epithelial response to infection. Veterinary Immunology and Immunopathology, 101, 191-202. Go to original source... Go to PubMed...
    38. Zhao G.Q., Guo Y.H., Sun L.S. (2008): Study on vitamin A optimum requirements of lactating Holsteins. Chinese Dairy Cattle, 9, 7-10. (in Chinese)

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content