Czech J. Anim. Sci., 2015, 60(5):216-224 | DOI: 10.17221/8171-CJAS

Assessment of genetic relationship between six populations of Welsh Mountain sheep using microsatellite markersOriginal Paper

K.M. Huson, W. Haresign, M.J. Hegarty, T.M. Blackmore, C. Morgan, N.R. McEwan
Institute of Biological, Environmental and Rural Sciences, Penglais Campus, Aberystwyth University, Aberystwyth, United Kingdom

This study investigated the genetic relationship between 6 populations of Welsh Mountain sheep: 5 phenotypic breed-types within the Welsh Mountain (WM) sheep breed, which have each been bred in specific geographic areas of Wales, and the Black Welsh Mountain sheep breed. Based on DNA analysis using 8 microsatellite markers, observed heterozygosity levels were similar to those expected in livestock populations subjected to selective breeding (0.530-0.664), and all but one population showed evidence of inbreeding. Using Bayesian cluster and Neighbor-joining analyses, the Black Welsh Mountain sheep were identified as being the outlier group, and the remaining groups could be categorized into five distinct sub-populations, which reflects the geographical separation seen between these populations.

Keywords: sheep; interbreed relationship; population biology; phylogeography

Published: May 31, 2015  Show citation

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Huson KM, Haresign W, Hegarty MJ, Blackmore TM, Morgan C, McEwan NR. Assessment of genetic relationship between six populations of Welsh Mountain sheep using microsatellite markers. Czech J. Anim. Sci. 2015;60(5):216-224. doi: 10.17221/8171-CJAS.
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    References

    1. Agaoglu O.K., Ertugrul O. (2012): Assessment of genetic diversity, genetic relationship and bottleneck using microsatellites in some native Turkish goat breeds. Small Ruminant Research, 105, 53-60. Go to original source...
    2. Alvarez I., Capote J., Traore A., Fonseca N., Perez K., Cuervo M., Fernandez I., Goyache F. (2012): Genetic relationships of the Cuban hair sheep inferred from microsatellite polymorphism. Small Ruminant Research, 104, 89-93. Go to original source...
    3. Arranz J., Bayon Y., San Primitivo F. (2001): Differentiation among Spanish sheep breeds using microsatellites. Genetic Selection and Evolution, 33, 529-542. Go to original source... Go to PubMed...
    4. Blackburn H., Paiva S., Wildeus S., Getz W., Waldron D., Stobart R., Bixby D., Purdy P., Welsh C., Spiller S., Brown M. (2011): Genetic structure and diversity among sheep breeds in the United States: identification of the major gene pools. Journal of Animal Science, 89, 2336-2348. Go to original source... Go to PubMed...
    5. Bowles D., Carson A., Isaac P. (2014): Genetic distinctiveness of the Herdwick Sheep breed and two other locally adapted hill breeds of the UK. PLOS ONE, 9, e87823. Go to original source... Go to PubMed...
    6. Bozzi R., Degl'Innocenti P., Rivera Diaz P., Nardi L., Crovetti A., Sargentini C., Giorgetti A. (2009): Genetic characterization and breed assignment in five Italian sheep breeds using microsatellite markers. Small Ruminant Research, 85, 50-57. Go to original source...
    7. Carrillo J.A., Siewerdt F. (2010): Consequences of longterm inbreeding accumulation on preweaning traits in a closed nucleus Angus herd. Journal of Animal Science, 88, 87-95. Go to original source... Go to PubMed...
    8. Carson A., Elliot M., Groom J., Winter A., Bowles D. (2009): Geographical isolation of native sheep breeds in the UK - evidence of endemism as a risk factor to genetic resources. Livestock Science, 123, 288-299. Go to original source...
    9. Ciani E., Ciampolini R., D'Andrea M., Castellana E., Cecchi F., Incoronato C., d'Angelo F., Albenzio M., Pilla F., Matassino D., Cianci D. (2013): Analysis of genetic variability within and among Italian sheep breeds reveals population stratification and suggests the presence of a phylogeographic gradient. Small Ruminant Research, 112, 21-27. Go to original source...
    10. DEFRA (2003): The breeding structure of the British sheep industry 2003. DEFRA Publications, UK.
    11. DEFRA (2014): Revised approved list of UK native breeds at risk. DEFRA Publications, UK.
    12. Dalvit C., Savva E., Cassandro M., Gervaso M., Pastore E., Piasentier E. (2008): Genetic diversity and variability in Alpine sheep breeds. Small Ruminant Research, 80, 45-51. Go to original source...
    13. Dixit S., Verma N., Aggarwal R., Vyas M., Rana J., Sharma A. (2011): Genetic diversity and relationship among Indian goat breeds based on microsatellite markers. Small Ruminant Research, 105, 38-45. Go to original source...
    14. Duckham A.N. (1963): Farming. Volume 2. The Caxton Publishing, London, UK.
    15. FAO (2011): Molecular genetic characterization of animal genetic resources. FAO Animal Production and Health Guidelines. FAO, Rome, Italy.
    16. Granevitze Z., Hillel J., Chen G.H., Cuc N.T.K., Feldman M., Eding H., Weigend S. (2007): Genetic diversity within chicken populations from different continents and management histories. Animal Genetics, 38, 576-583. Go to original source... Go to PubMed...
    17. Groeneveld L.F., Lenstra J.A., Eding H., Toro M.A., Scherf B., Pilling D., Negrini R., Finlay E.K., Jianlin H., Groeneveld E., Weigend S., GLOBALDIV Consortium (2010): Genetic diversity in farm animals - a review. Animal Genetics, 41, 6-31. Go to original source... Go to PubMed...
    18. Hoffmann I., Ajmone Marsan P., Barker J.S.F., Cothran E.G., Hanotte O., Lenstra J.A., Milan D., Weigend S., Simianer H. (2004): New MoDAD marker sets to be used in diversity studies for the major farm animal species: recommendations of a joint ISAG/FAO working group. In: Proc. 29th Internat. Conference on Animal Genetics, Tokyo, Japan, 123.
    19. Hybu Cig Cymru (2014): Little Book of Meat Facts: Compendium of Welsh Red Meat and Livestock Industry Statistics 2014. HCC-MPW Publications, Aberystwyth, UK.
    20. Ibrahim M., Ahmed S., Swati Z., Kahn M. (2010): Genetic diversity in Balkhi, Hashtnagri and Michni sheep populations using SSR markers. African Journal of Biotechnology, 9, 7617-7628.
    21. Lawson Handley L.J., Byrne K., Santucci F., Townsend S., Taylor M., Bruford M.W., Hewitt G.M. (2007): Genetic structure of European sheep breeds. Heredity, 99, 620-631. Go to original source... Go to PubMed...
    22. Ligda Ch., Altarayrah J., Georgoudis A., ECONOGENE Consortium (2009): Genetic analysis of Greek sheep breeds using microsatellite markers for setting conservation priorities. Small Ruminant Research, 83, 42-48. Go to original source...
    23. McParland S., Kearney J.F., Rath M., Berry D.P. (2007): Inbreeding effects on milk production, calving performance, fertility, and conformation in Irish HolsteinFriesians. Journal of Dairy Science, 90, 4411-4419. Go to original source... Go to PubMed...
    24. Medugorac I., Medugorac A., Russ I., Veit-Kensch C.E., Taberlet P., Luntz B., Mix H.M., Forster M. (2009): Genetic diversity of European cattle breeds highlights the conservation value of traditional unselected breeds with high effective population size. Molecular Ecology, 18, 3394-3410. Go to original source... Go to PubMed...
    25. Notter D. (1999): The importance of genetic diversity in livestock populations of the future. Journal of Animal Science, 77, 61-69. Go to original source... Go to PubMed...
    26. NSA (1998): British Sheep. National Sheep Association, Malvern, UK.
    27. Pritchard J., Stephens M., Donnelly P. (2000): Inference of population structure using multilocus genotype data. Genetics, 155, 945-959. Go to original source... Go to PubMed...
    28. Rendo F., Iriondo M., Jugo B., Mazon L., Aguirre A., Vicario A., Estonba A. (2004): Tracking diversity and differentiation in six sheep breeds from the North Iberian Peninsula through DNA variation. Small Ruminant Research, 52, 195-202. Go to original source...
    29. Roberts J.A.F., White R.G. (1930): Colour inheritance in sheep. V. Dominant black. Journal of Genetics, 22, 181-190. Go to original source...
    30. SanCristobal M., Chevalet C., Haley C.S., Joosten R., Rattink A.P., Harlizius B., Groenen M.A.M, Amigues Y., Boscher M.Y., Russell G., Law A., Davoli R., Russo V., Desautes C., Alderson L., Fimland E., Bagga M., Delgado J.V., Vega-Pla J.L., Martinez A.M., Ramos M., Glodek P., Meyer J.N., Gandini G.C., Matassino D., Plastow G.S., Siggens K.W., Laval G., Archibald A.L., Milan D., Hammond K., Cardellino R. (2006): Genetic diversity within and between European pig breeds using microsatellite markers. Animal Genetics, 37, 189-198. Go to original source... Go to PubMed...
    31. Wood R. (1937): Survey of sheep management in Great Britain: Volume 3 of Reports of the college travelling scholarships in agriculture. College of Estate Management, Reading, UK.

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