Czech J. Anim. Sci., 2011, 56(3):107-113 | DOI: 10.17221/1299-CJAS

The association between microsatellite Bm6438 and milk performance traits in Polish Holstein-Friesian cattle

T. Zabolewicz1, U. Czarnik1, J. Strychalski1, C.S. Pareek2, M. Pierzchała3
1 Department of Animal Genetics, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland
2 Laboratory of Functional Genomics, Institute of General and Molecular Biology, Faculty of Biology and Earth Science, Nicolaus Copernicus University, Toruń, Poland
3 Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzębiec, Poland

The objective of this study was to verify the hypothesis postulating the location of QTL linked marker for milk performance traits in the proximal section of chromosome BTA1 by analyzing the microsatellite BM6438 alleles from heterozygous sires and the milk performance traits of the investigated cattle. The experiment covered 484 Polish Holstein-Friesian primiparous cows, the progeny of three unrelated sires, including 317 cows ‒ the progeny of two sires with 256/268 genotype and 167 cows - the progeny of a sire with 258/268 genotype. BM6438 polymorphism was determined by PCR and polyacrylamide gel electrophoresis. Three alleles (256, 258 and 268), forming 6 genotype groups, were identified among the studied cattle. In the progeny of sires with 256/268 genotype, statistically significant differences were found out in protein yield (the progeny of 2 sires) and in milk yield (the progeny of 1 sire). In both cases, the highest values of performance traits were recorded in 268/268 genotype groups, and the lowest in half-sib groups with 256/258 and 258/268 genotypes. The analysis covering the progeny of two sires with 256/268 genotype confirmed the regularities noted in individual families. 268/268 homozygotes had a highly significantly higher (P ≤ 0.01) protein yield than the remaining genotype groups. Statistically significant differences were also reported in the values of milk yield (P ≤ 0.05).

Keywords: cattle; Polish Holstein-Friesian breed; BM6438 polymorphism; milk performance traits

Published: March 31, 2011  Show citation

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Zabolewicz T, Czarnik U, Strychalski J, Pareek CS, Pierzchała M. The association between microsatellite Bm6438 and milk performance traits in Polish Holstein-Friesian cattle. Czech J. Anim. Sci. 2011;56(3):107-113. doi: 10.17221/1299-CJAS.
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    References

    1. Ashwell M.S., Heyen D.W., Sonstegard T.S., Van Tassell C.P., Da Y., VanRaden P.M., Ron M., Weller J.I., Lewin H.A. (2004): Detection of quantitative trait loci affecting milk production, health, and reproductive traits in Holstein cattle. Journal of Dairy Science, 87, 468-475. Go to original source... Go to PubMed...
    2. Bagnato A., Schiavini F., Rossoni A., Maltecca C., Dolezal M., Medugorac I., Sölkner J., Russo V., Fontanesi L., Friedmann A., Soller M., Lipkin E. (2008): Quantitative trait loci affecting milk yield and protein percentage in a three-country Brown Swiss population. Journal of Dairy Science, 91, 767-783. Go to original source... Go to PubMed...
    3. Bishop M.D., Kappes S.M., Keele J.W., Stone R.T., Sunden S.L., Hawkins G.A., Toldo S.S., Fries R., Grosz M.D., Yoo J., Beattie C.W. (1994): A genetic linkage map for cattle. Genetics, 136, 619-639. Go to original source... Go to PubMed...
    4. Blott S., Kim J.J., Moisio S., Schmidt-Kuntzel A., Cornet A., Berzi P., Cambisano N., Ford C., Grisart B., Johnson D., Karim L., Simon P., Snell R., Spelman R., Wong J., Vilkki J., Georges M., Farnir F., Coppieters W. (2003): Molecular dissection of a quantitative trait locus: a phenylalanine-to-tyrosine substitution in the transmembrane domain of the bovine growth hormone receptor is associated with a major effect on milk yield and composition. Genetics, 163, 253-266. Go to original source... Go to PubMed...
    5. Cohen-Zinder M., Seroussi E., Larkin D.M., Loor J.J., Evertsvan der Wind A., Lee J.H., Drackley J.K., Band M.R., Hernandez A.G., Shani M., Lewin H.A., Weller J.I., Ron M. (2005): Identification of a missense mutation in the bovine ABCG2 gene with a major effect on the QTL on chromosome 6 affecting milk yield and composition in Holstein cattle. Genome Research, 15, 936-944. Go to original source... Go to PubMed...
    6. Czarnik U., Walawski K., Zabolewicz T., Pareek C.S. (2005): Preliminary evaluation of BM6438 polymorphism as a putative candidate marker for milk performance traits associated with a new breeding concept in Polish Black-and-White cattle. Annals of Animal Science, 5, 243-251.
    7. Czarnik U., Galiński M., Zabolewicz T., Pareek C.S. (2007): Study of an association between SNP 775C>T within the bovine ITBG2 gene and milk production traits in Black-and-White cows. Czech Journal of Animal Science, 52, 1-6. Go to original source...
    8. Georges M., Nielsen D., Mackinnon M., Mishra A., Okimoto R., Pasquino A.T., Sargeant L.S., Sorensen A., Steele M.R., Zhao X., Womack J.E., Hoeschele I. (1995): Mapping quantitative traits loci controlling milk production in dairy cattle by exploiting progeny testing. Genetics, 139, 907-920. Go to original source... Go to PubMed...
    9. Gutiérrez-Gil B., Ball N., Burton D., Haskell M., Williams J.L., Wiener P. (2008): Identification of quantitative trait loci affecting cattle temperament. Journal of Heredity, 99, 629-638. Go to original source... Go to PubMed...
    10. Khatkar M.S., Thomson P.C., Tammen I., Raadsma H.W. (2004): Quantitative trait loci mapping in dairy cattle: review and meta-analysis. Genetics, Selection, Evolution, 36, 163-190. Go to original source... Go to PubMed...
    11. Lund M.S., Guldbrandtsen B., Buitenhuis A.J., Thomsen B., Bendixen C. (2008): Detection of quantitative trait loci in Danish Holstein cattle affecting clinical mastitis, somatic cell score, udder conformation traits, and assessment of associated effects on milk yield. Journal of Dairy Science, 91, 4028-4036. Go to original source... Go to PubMed...
    12. Mosig M.O., Lipkin E., Khutoreskaya G., Tchourzyna E., Soller M., Friedmann A. (2001): A whole genome scan for quantitative trait loci affecting milk protein percentage in Israeli-Holstein cattle, by means of selective milk DNA pooling in a daughter design, using an adjusted false discovery rate criterion. Genetics, 157, 1683-1698. Go to original source... Go to PubMed...
    13. Nadesalingam J., Plante Y., Gibson J.P. (2001): Detection of QTL for milk production on Chromosomes 1 and 6 of Holstein cattle. Mammalian Genome, 12, 27-31. Go to original source... Go to PubMed...
    14. Polineni P., Aragonda P., Xavier S.R., Furuta R., Adelson D.L. (2006): The bovine QTL viewer: a web accessible database of bovine Quantitative Trait Loci. BMC Bioinformatics, 7, 283. Go to original source... Go to PubMed...
    15. Rodriguez-Zas S.L., Southey B.R., Heyen D.W., Lewin H.A. (2002): Interval and composite interval mapping of somatic cell score, yield, and components of milk in dairy cattle. Journal of Dairy Science, 85, 3081-3091. Go to original source... Go to PubMed...
    16. Ron M., Feldmesser E., Golik M., Tager-Cohen I., Kliger D., Reiss V., Domochovsky R., Alus O., Seroussi E., Ezra E., Weller J.I. (2004): A complete genome scan of the Israeli Holstein population for quantitative trait loci by a daughter design. Journal of Dairy Science, 87, 476-490. Go to original source... Go to PubMed...
    17. Schnabel R.D., Kim J.J., Ashwell M.S., Sonstegard T.S., Van Tassell C.P., Connor E.E., Taylor J.F. (2005): Finemapping milk production quantitative trait loci on BTA6: Analysis of the bovine osteopontin gene. Proceedings of the National Academy of Sciences of the USA, 102, 6896-6901. Go to original source... Go to PubMed...
    18. Schrooten C., Bink M.C., Bovenhuis H. (2004): Whole genome scan to detect chromosomal regions affecting multiple traits in dairy cattle. Journal of Dairy Science, 87, 3550-3560. Go to original source... Go to PubMed...
    19. Winter A., Kramer W., Werner F.A., Kollers S., Kata S., Durstewitz G., Buitkamp J., Womack J.E., Thaller G., Fries R. (2002): Association of a lysine-232/alanine polymorphism in a bovine gene encoding acyl-CoA: diacylglycerol acyltransferase (DGAT1) with variation at a quantitative trait locus for milk fat content. Proceedings of the National Academy of Sciences of the USA, 99, 9300-9305. Go to original source... Go to PubMed...
    20. Zhang Q., Boichard D., Hoeschele I., Ernst C., Eggen A., Murkve B., Pfister-Genskow M., Witte L.A., Grignola F.E., Uimari P., Thaller G., Bishop M.D. (1998): Mapping quantitative trait loci for milk production and health of dairy cattle in a large outbreed pedigree. Genetics, 149, 1959-1973. Go to original source... Go to PubMed...

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