Czech J. Anim. Sci., 2010, 55(1):37-41 | DOI: 10.17221/1709-CJAS

A novel porcine gene, USP7, differentially expressed in the musculus longissimus from Wujin and Large White pigs

Liu Yongganmg, Gao Shizheng
Key Laboratory of Animal Nutrition and Feed of Yunnan Province, Yunnan Agricultural University, Kunming, P.R. China

The mRNA differential display technique was performed to investigate differences in the gene expression in the musculus longissimus from Wujin and Large White pigs. A fragment of one differentially expressed gene was isolated and sequenced. A complete cDNA sequence was obtained using the rapid amplification of cDNA ends (RACE) method. The sequence prediction analysis revealed that the open reading frame of this gene encodes a protein of 1 103 amino acids, which is homologous with the ubiquitin specific peptidase 7 (USP7) of four species, rat (identity 98%), human (98%), mouse (98%) and chicken (95%), so it can be defined as the porcine USP7 gene. The differences in the USP7 gene expression in muscles from Wujin and Large White pigs were confirmed using semi-quantitative RT-PCR. The gene expression analysis in eight tissues of a Wujin × Large White cross showed that USP7 was expressed in all the tissues, except for fat.

Keywords: pig; mRNA differential display; RACE; USP7 gene

Published: January 31, 2010  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Yongganmg L, Shizheng G. A novel porcine gene, USP7, differentially expressed in the musculus longissimus from Wujin and Large White pigs. Czech J. Anim. Sci. 2010;55(1):37-41. doi: 10.17221/1709-CJAS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Bílek K., Knoll A., Stratil A., Svobodová K., Horák P., Bechyňová R., Van Poucke M., Peelman L.J. (2008): Analysis of mRNA expression of CNN3, DCN, FBN2, POSTN, SPARC and YWHAQ genes in porcine foetal and adult skeletal muscles. Czech Journal of Animal Science, 53, 181-186. Go to original source...
    2. D'Andrea A., Pellman D. (1998): Deubiquitinating enzymes: a new class of biological regulators. Critical Reviews in Biochemistry and Molecular Biology, 33, 337-352. Go to original source... Go to PubMed...
    3. Hong S., Kim S.J., Ka S., Choi I., Kang S. (2002): USP7, a ubiquitin-specific protease, interacts with ataxin-1, the SCA1 gene product. Molecular and Cellular Neuroscience, 20, 298-306. Go to original source... Go to PubMed...
    4. Li M., Chen D., Shiloh A., Luo J., Nikolaev A.Y., Qin J., Gu W. (2002): Deubiquitination of p53 by HAUSP is an important pathway for p53 stabilization. Nature, 416, 648-653. Go to original source... Go to PubMed...
    5. Liang P., Pardee A.B. (1992): Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science, 257, 967-971. Go to original source... Go to PubMed...
    6. Liang P., Averboukh L., Pardee A.B. (1993): Distribution and cloning of eukaryotic mRNAs by means of differential display: Refinements and optimization. Nucleic Acids Research, 21, 3269-3275. Go to original source... Go to PubMed...
    7. Lin C.S., Hsu C.W. (2005): Differentially transcribed genes in skeletal muscle of Duroc and Taoyuan pigs. Journal of Animal Science, 83, 2075-2086. Go to original source... Go to PubMed...
    8. Liu G.Y., Xiong Y.Z. (2007): Isolation, sequence analysis and expression profile of a novel porcine gene, NIP7, differentially expressed in the backfat tissues from Meishan, Meishan × Large White Cross and Large White Pigs. Molecular Biology Reports, 34, 213-219. Go to original source... Go to PubMed...
    9. Liu G.Y., Xiong Y.Z., Deng C.Y., Zuo B., Zhang J.H. (2004): Comparison of gene expression patterns in Longissimus dorsi of pigs between the high-parent heterosis cross combination Landrace × Large White and the mid-parent heterosis cross combination Large White × Meishan. Asian-Australasian Journal of Animal Science, 17, 1192-1196. Go to original source...
    10. Liu G.Y., Xiong Y.Z., Zuo B., Jiang S.W., Deng C.Y., Li J. L., Li F.E., Zheng R. (2006): Isolation, identification and expression profile of fourteen differentially expressed sequence tags in the longissimus dorsi muscle tissues from Meishan, Meishan × Large White cross and Large White pigs. Chinese Journal of Agricultural Biotechnology, 4, 9-14. Go to original source...
    11. Pan P.W., Zhao S.H., Yu M., Xiong T.A., Li K. (1993): Identification of differentially expressed genes in the Longissimus dorsi tissue between Duroc and Erhualian pigs by mRNA differential display. Asian-Australasian Journal of Animal Science, 16, 1066-1070. Go to original source...
    12. Yamazaki M., Saito K. (2002): Differential display analysis of gene expression in plants. Cellular and Molecular Life Sciences, 59, 1246-1255. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content