Czech J. Anim. Sci., 2009, 54(5):210-216 | DOI: 10.17221/1663-CJAS

Missense mutations in exon 2 of the porcine leptin receptor gene and their associations with litter size and body weight

C. Sun, L. Wang, D. F. Jiang, B. Zhang
College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi, China

Leptin receptor (LEPR) gene is regarded as a "candidate-gene" of production traits. The aims of this study were to detect polymorphisms of exon 2 within LEPR gene and to investigate their associations with production traits, litter size and live weight in Luchuan and Large White pig breeds. For this purpose, the single nucleotide polymorphisms (SNPs) of exon 2 within LEPR were detected using the PCR-SSCP procedure and their association with litter size and live weight was also analysed in Luchuan (n = 446) and Large White pigs (n = 405). The results showed that the C155T mutation was found in exon 2 of porcine LEPR gene in analyzed populations, which caused a missense mutation (Met to Thr). The different genotypes of this locus had the effects on total number of piglets born, born alive of the first (1st) and the first to the fourth (1st-4th) litters. The individuals with allele A had a higher number of total piglets born and a higher number of born alive piglets in the first litter (P < 0.05) while the differences in birth weight, weaning weight and average daily gain were not significant among different genotypes of this locus in analyzed populations. It implies that the exon 2 (Thr/Met) mutation of LEPR gene is a potential gene marker of pig reproduction. Therefore, it can be used in the marker-assisted selection (MAS) of pig breeding work.

Keywords: LEPR; SNPs; pigs; litter size; live weight

Published: May 31, 2009  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Sun C, Wang L, Jiang DF, Zhang B. Missense mutations in exon 2 of the porcine leptin receptor gene and their associations with litter size and body weight. Czech J. Anim. Sci. 2009;54(5):210-216. doi: 10.17221/1663-CJAS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Avcin T., Tse S.M., Schneider R., Ngan B., Silverman E.D. (2006): Macrophage activation syndrome as the presenting manifestation of rheumatic diseases in childhood. The Journal of Pediatrics, 148, 683-686. Go to original source... Go to PubMed...
    2. Chen C.C., Chang T., Su H.Y. (2004): Characterization of porcine leptin receptor polymorphisms and their association with reproduction and production traits. Animal Biotechnology, 15, 89-102. Go to original source... Go to PubMed...
    3. Chen S.C., Cunningham J.J., Smeyne R.J. (2000): Expression of OB receptor splice variants during prenatal development of the mouse. Journal of Receptor and Signal. Transduction Research, 20, 87-103. Go to original source... Go to PubMed...
    4. Chmurzynska A., Mackowski M., Szydowski M., Melonek J., Kamyczek M., Eckert R., Rózycki M., Świtonski M. (2004): Polymorphism of intronic microsatellites in the A-FABP and LEPR genes and its association with productive traits in the pig. Journal of Animal and Feed Sciences, 13, 615-624. Go to original source...
    5. Chua S.C. Jr., Chung W.K., Wu-Peng X.S., Zhang Y., Liu S-M., Leibel R.L. (1996): Phenotypes of mouse diabetes and rat fatty due to mutations in the OB (leptin) receptor. Science, 271, 994-996. Go to original source... Go to PubMed...
    6. de Luis Roman D., de la Fuente R., Sagrado M., Izaola O., Vicente R. (2006): Leptin receptor Lys656Asn polymorphism is associated with decreased leptin response and weight loss secondary to a lifestyle modification in obese patients. Archives of Medical Research, 37, 854-859. Go to original source... Go to PubMed...
    7. Ernst C.W., Kapke P.A., Yerle M., Rothschild M.F. (1997): The leptin receptor gene (LEPR) maps to porcine chromosome 6. Mammalian Genome, 8, 266-272. Go to original source... Go to PubMed...
    8. Jin L., Zhang S., Burguera B.G., Couce M.E., Osamura R.Y., Kuling E., Lloyd R.V. (2000): Leptin and leptin receptor expression in rat and mouse pituitary cells. Endocrinology, 141, 333-339. Go to original source... Go to PubMed...
    9. Kojima C.J., Carroll J.A., Matteri R.L., Touchette K.J., Allee G.L. (2007): Effects of weaning and weaning weight on neuroendocrine regulators of feed intake in pigs. Journal of Animal Science, 85, 2133-2139. Go to original source... Go to PubMed...
    10. Komisarek J., Dorynek Z. (2006): The relationship between the T945M single nucleotide polymorphism in the leptin receptor gene (LEPR) and milk production traits in Jersey cows. Animal Science Papers and Reports, 24, 271-277.
    11. Kopečný M., Stratil A., Čepica S. (1997): Polymorphisms at the porcine LEPR gene detected by PCR-DGGE. Animal Genetics, 28, 461-467. Go to original source... Go to PubMed...
    12. Liefers S.C., te Pas M.F.W., Veerkamp R.F., van der Lende T. (2002): Associations between leptin gene polymorphisms and production, live weight, energy balance, feed intake, and fertility in Holstein heifers. Journal of Dairy Science, 85, 1633-1638. Go to original source... Go to PubMed...
    13. Mackowski M., Szymoniak K., Szydlowski M., Kamyczek M., Eckert R., Rozycki M., Switonski M. (2005): Missense mutations in exon 4 of the porcine LEPR gene encoding extracellular domain and their association with fatness traits. Animal Genetics, 36, 135-137. Go to original source... Go to PubMed...
    14. Moritani M., Nomura K., Tanahashi T., Osabe D., Fujita Y., Shinohara S., Yanaguchi Y., Keshavarz P., Kudo E., Nakamura N., Yoshikawa T., Ichiishi E., Takata Y., Yasui N., Shiota H., Kunika K., Inoue H., Itakura M. (2007): Genetic association of single nucleotide polymorphisms in endonuclease G-like 1 gene with type 2 diabetes in a Japanese population. Diabetologia, 50, 1218-1227. Go to original source... Go to PubMed...
    15. Nephawe K.A., Spurlock D.M. (2007): Single nucleotide polymorphisms in five adipokine genes in dairy cattle populations. South African Journal of Animal Science, 37, 154-157 Go to original source...
    16. Olivo C., Fernandez A., Noguera J., Barragan C., Leton R., Rodriguez C., Mercade A., Alves E., Folch J.M., Varona L., Toro M. (2005): Fine mapping of porcine chromosome 6 QTL and LEPR effects on body composition in multiple generations of an Iberian by Landrace intercross. Genetical Research, 85, 57-67. Go to original source... Go to PubMed...
    17. Park K.S., Shin H.D., Park B.L., Cheong H.S., Cho Y.M., Lee H.K., Lee J.K., Oh B., Kimm K. (2006): Polymorphisms in the leptin receptor-putative association with obesity and T2DM. Journal of Human Genetics, 51, 85-91 Go to original source... Go to PubMed...
    18. Sawhney S., Woo P., Murray K.J. (2001): Macrophage activation syndrome: a potentially fatal complication of rheumatic disorders. Archives of Disease in Childhood, 85, 421-426. Go to original source... Go to PubMed...
    19. Schenkel F.S., Miller S.P., Moore S.S., Li C., Fu A., Lobo S., Mandell I.B., Wilton J.W. (2006): Association of SNPs in the leptin and leptin receptor genes with different fat depots in beef cattle. In: Proceedings of the 8th World Congress on Genetics Applied to Livestock Production, Instituto Prociencia, Minas Gerais, Brazil.
    20. Tartaglia L.A., Dembski M., Weng X., Deng N., Culpepper J., Devos R., Richard G.J., Campfield L.A., Clark F.T., Deeds J., Muir C., Sanker S., Moriarty A., Moore K.J., Smutko J.S., Mays G.G., Wool E.A., Monroe C.A., Tepper R.I. (1995): Identification and expression cloning of a leptin receptor. OB-R. Cell, 83, 1263-1271. Go to original source... Go to PubMed...
    21. van der Vleuten G.M., Kluijtmans L.A., Hijman A., Blom H.J., Stalenhoef A.F., de Graaf J. (2006): The Gln223Arg polymorphism in the leptin receptor is associated with familial combined hyperlipidemia. International Journal of Obesity, 30, 892-898. Go to original source... Go to PubMed...
    22. Vincent A.L., Wang L., Rothschild M.F. (1997): Rapid communication: restriction fragment length polymorphism in the porcine leptin receptor (LEPR) gene. Journal of Animal Science, 75, 2287. Go to original source... Go to PubMed...
    23. Xie D.G., Qiu L.T. (2003): The Conservation and selection report of Luchuan pig. Guangxi Journal of Animal Husbandry and Veterinary Medicine, 19, 76-78.
    24. Zhang Y.Y., Mlynarski W., Frazier W., Nolan D., Duffy J., Marescoffi M.C., Gervino E.V., Johnstone M.T., Mantzoros C.S., Avogaro A., Doria A. (2007): Genetic variability at the leptin receptor (LEPR) locus is a determinant of plasma fibrinogen and C-reactive protein levels. Atherosclerosis, 191, 121-127. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content