Czech J. Anim. Sci., 2008, 53(6):246-252 | DOI: 10.17221/363-CJAS

Joint effects of CSN3 and LGB genes on milk quality and coagulation properties in Czech Fleckvieh

A. Matějíček1, J. Matějíčková1, M. Štípková1, O. Hanuš2, V. Genčurová2, J. Kyseľová1, E. Němcová1, T. Kott1, J. Šefrová1, M. Krejčová1, S. Melčová1, I. Hölzelová1, J. Bouška1, J. Frelich3
1 Institute of Animal Science, Prague-Uhříněves, Czech Republic
2 Research Institute for Cattle Breeding, Rapotín, Czech Republic
3 University of South Bohemia, České Budějovice, Czech Republic

The aim of this study was to determine the joint effects of CSN3 and LGB genotypes on parameters of production, quality and coagulation of milk in Czech Fleckvieh cows. Three hundred and twenty-eight Czech Fleckvieh cows were determined for CSN3 (kappa-casein) and LGB (beta-lactoglobulin) genotypes using the PCR-RFLP method, milk quality parameters and coagulation properties. Milk production parameters were obtained from the Official Database of Progeny Testing. Fifteen genotype combinations were detected, with ABAB (21.0%) and AAAB (18.3%) occurring as the most frequent. The observed genes significantly affected the contents of milk protein (crude protein, true protein, casein and whey protein) as well as solid non-fat in milk, casein number and curd quality. BBAA was found to be the genotype with the highest positive impact on most of the milk characteristics evaluated. Whereas ABBB, BBBB, BBAB and ABAB had a positive influence on milk quality and milk coagulation properties, genotypes containing CSN3 allele E had a negative effect. Results presented in this study are applicable in the selection of Czech Fleckvieh cattle.

Keywords: cattle; milk protein genes; kappa-casein; beta-lactoglobulin; milk production parameters; coagulation properties

Published: June 30, 2008  Show citation

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Matějíček A, Matějíčková J, Štípková M, Hanuš O, Genčurová V, Kyseľová J, et al.. Joint effects of CSN3 and LGB genes on milk quality and coagulation properties in Czech Fleckvieh. Czech J. Anim. Sci. 2008;53(6):246-252. doi: 10.17221/363-CJAS.
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    References

    1. Agrawala P.L., Wagner V.A., Geldermann H. (1992): Sex determination and milk protein genotyping of preimplantation stage bovine embryos using multiplex PCR. Theriogenology, 38, 969-978. Go to original source... Go to PubMed...
    2. Amigo L., Martin-Alvarez P.J., Garcia-Muro E., Zarazaga I. (2001): Effect of milk protein haplotypes on the composition and technological properties of Fleckvieh bovine milk. Milchwissenschaft, 56, 488-491.
    3. Aschaffenburg R., Drewry J. (1955): Occurrence of different beta-lactoglobulins in cow's milk. Nature, 176, 218 pp. Go to original source... Go to PubMed...
    4. Comin A., Cassandro M., Ojala M., Bittante G. (2006): Effect of β- and κ-casein genotypes on milk coagulation properties, milk production and content, and milk quality traits in Italian Holstein cows. In: 57th Annual Meeting of the EAAP, 17.-20.9. 2006, Antalya, Turkey.
    5. ČSN 57 0530 (1974): Methods for testing of milk and milk products. Czech Standards Institute, Prague, CR.
    6. ČSN 57 0536 (1999): determination of milk composition by mid-infrared analyser. Czech Standards Institute, Prague, CR.
    7. Eigel W.N., Butler J.E., Ernstrom C.A., Farrell H.M., Harwalkar V.R., Jennes R., Whitney R.McL. (1984): Nomenclature of proteins of cow's milk. J. Dairy Sci., 67, 1599-1631. Go to original source...
    8. Erhardt G. (1989): κ-caseins in bovine milk. Evidence of a further allele (kappa;-Cn E) in different breeds. J. Anim. Breed. Genet., 106, 225-231. Go to original source...
    9. Farrell H.M., Jimenez-Flores R., Bleck G.T., Brown E.M., Butler J.E., Creamer L.K., Hicks C.L., Hollar C.M., NgKwai-Hang K.F., Swaisgood H.E. (2004): Nomenclature of the proteins of cows' milk - sixth revision. J. Dairy Sci., 87, 1641-1674. Go to original source... Go to PubMed...
    10. Hanuš O., Gajdůšek S., Gabriel B., Kopecký J., Jedelská R. (1995): Cheese-making properties of raw and pasteurized milk with respect to milk protein polymorphism. Czech J. Anim. Sci., 40, 523-528.
    11. Hanuš O., Beber K., Kopecký J. (2000): Milk protein variants and characteristics of cows, milk. In: Breeding, Nutritional and Technological Aspects of Milk Production and Quality. Rapotín, Czech Republic, 47-49.
    12. Choi J.W., Ng-Kwai-Hang K.F. (2002): Effects of genetic variants of κ-casein and β-lactoglobulin and heat treatment of milk on cheese and whey compositions. AsianAust. J. Anim. Sci., 5, 732-739. Go to original source...
    13. Ikonen T., Ahlfors K., Kempe R., Ojala M., Ruttionen O. (1999a): Genetic parameters for milk coagulation properties and prevalence of noncoagulating milk in Finnish dairy cows. J. Dairy Sci., 82, 205-214. Go to original source... Go to PubMed...
    14. Ikonen T., Ojala M., Ruottinen O. (1999b): Associations between milk protein polymorphism and first lactation milk production traits in Finnish Ayrshire cows. J. Dairy Sci., 82, 1026-1033. Go to original source... Go to PubMed...
    15. Ikonen T., Bovenhuis H., Ojala M., Ruottinen O., Georges M. (2001): Associations between casein haplotypes and first lactation milk production traits in Finnish Ayrshire cows. J. Dairy Sci., 84, 507-514. Go to original source... Go to PubMed...
    16. Kaminski S., Rymkiewicz-Schymczyk J., Wojcik E., Rusc A. (2002): Associations between bovine milk protein genotypes and haplotypes and the breeding value of Polish Black-and-White bulls. J. Anim. Feed Sci., 11, 205-221. Go to original source...
    17. Kawasaki E.S. (1990): Sample preparation from blood, cells and another fluids. In: PCR Protocols: A Guide to Methods and Applications. Academic Press, New York, USA, 146-152. Go to original source...
    18. Kučerová J., Němcová E., Štípková M., Jandurová O., Matějíček A., Bouška J. (2005): The association between CSN3 genotypes and milk production parameters in Czech Pied Cattle. In: 56th Ann. Meet. EAAP, 5.-8.6. 2005, Uppsala, Sweden. Available at http://www.eaap.org/uppsala/Papers/added/G6.15_Jitka.pdf
    19. Kučerová J., Matějíček A., Jandurová O. M., Sorensen P., Němcová E., Štípková M., Kott T., Bouška J., Frelich J. (2006): Milk protein genes CSN1S1, CSN2, CSN3, LGB and their relation to genetic values of milk production parameters in Czech Fleckvieh. Czech J. Anim. Sci., 51, 241-247. Go to original source...
    20. Lien S., Rogne S. (1993): Bovine casein haplotypes: number, frequencies and applicability as genetic markers. Anim. Genet., 24, 373-376. Go to original source... Go to PubMed...
    21. Matějíček A., Matějíčková J., Němcová E., Jandurová O.M., Štípková M., Bouška J., Frelich J. (2007): Joint effects of CSN3 and LGB genotypes and their relation to breeding values of milk production parameters in Czech Fleckvieh. Czech J. Anim. Sci., 52, 83-87. Go to original source...
    22. Neelin J. M. (1964): Variants of κ-casein revealed by improved starch gel electrophoresis. J. Dairy Sci., 47, 506 pp. Go to original source...
    23. Neubauerová V. (2001): Detection of genetic markers and possibilities of their use in cattle and other subungulates. Thesis University of South Bohemia, Agricultural Faculty, České budějovice, CR, 211 pp.
    24. Panicke L., Freyer G., Erhardt G. (1996): Effekte der Milchproteinpolymorphismen auf die Leistung. In: Kolloquium Milchprotein und Proteinansatz, GraalMüritz, FBN Dummerstorf, Universität Rostock, Germany, 20 pp.
    25. Ron M., Yoffe O., Ezra E., Medrano J.F., Weller J.I. (1994): Determination of effects of milk protein genotype on production traits of Israeli Holsteins. J. Dairy Sci., 77, 1106-1113. Go to original source... Go to PubMed...
    26. Sabour M.P., Lin C.Y., Lee A.J., McAllister A.J. (1996): Association between milk protein variants and genetic values of Canadian Holstein bulls for milk yield traits. J. Dairy Sci., 79, 1050-1056. Go to original source... Go to PubMed...
    27. Strzalkowska N., Krzyzewski J., Zwierzchowski L., Ryniewicz Z. (2002): Effects of κ-casein and β-lactoglobulin loci polymorphism, cows' age, stage of lactation and somatic cell count on daily milk yield and milk composition in Polish Black-and-White cattle. Institute of Genetics and Animal Breeding, Jastrzebiec, Poland. Anim. Sci. Pap. Rep., 20, 21-35.

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