Czech J. Anim. Sci., 2008, 53(4):145-151 | DOI: 10.17221/374-CJAS

Embryo recovery from the oviduct in superovulated ewes: a method to improve MOET systems

J.P. Ramon-Ugalde1, J. Folch2, M.J. Cocero3, R.E. Piña-Aguilar4, J.L. Alabart2
1 Center of Ovine Selection and Reproduction, Technical Institute of Conkal; Conkal, Mexico
2 Department of Animal Production, Centro de Investigacion y Tecnologia Agroalimentaria de Aragon, Zaragoza, Spain
3 Department of Animal Reproduction, INIA; Madrid, Spain
4 Faculty of Medicine, Autonomous University of Yucatan; Merida, Mexico

The efficiency of embryo recovery in a superovulatory treatment was studied by perfusing the oviduct or the uterine horn in 3.5 and 7 days after sponge withdrawal, respectively. Eighty-four and seventy ewes of Aragonesa breed were used as embryo donors and receptors, respectively. The donors were distributed in 3 replications. The oestrus was synchronized with the insertion of FGA intravaginal sponges (30 mg) for 13 days. Six intramuscular injections of 18 mg pFSH were applied in decreasing doses at 12 hour intervals starting 48 hours after sponge withdrawal. The recovery rate (RR) (83.2 vs. 75.8%), the viability rate (VR) (73.5 vs. 47.2%) and the number of viable embryos (VE) per donor ewe (5.9 ± 0.79 vs. 3.0 ± 0.37) were higher (P < 0.01) in ewes whose perfusion was done directly in oviducts. The interval from sponge withdrawal to oestrus had no influence on RR in any groups; however it had an effect on the VR in 3.5 and 7 days old embryos. Embryos from ewes showing the oestrus within 20 hours after sponge withdrawal had a higher viability. On the other hand, using morphologic criteria, 26.5% and 52.7% of 3.5 and 7 days old embryos, respectively, were discarded before transfer. The percentage of ewes in each group that became pregnant was similar in both groups. We conclude that in superovulated ewes the embryo recovery rate, viability and number of viable embryos are higher in embryos recovered from the oviduct compared to those recovered from the uterus, without decreasing the conception rate and the viability rate is higher in ewes that show the oestrus 20 hours before sponge withdrawal.

Keywords: embryo transfer; MOET; oviduct; uterus; superovulated sheep

Published: April 30, 2008  Show citation

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Ramon-Ugalde JP, Folch J, Cocero MJ, Piña-Aguilar RE, Alabart JL. Embryo recovery from the oviduct in superovulated ewes: a method to improve MOET systems. Czech J. Anim. Sci. 2008;53(4):145-151. doi: 10.17221/374-CJAS.
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    References

    1. Alexander A. (1986): Técnica quirúrgica en animales y temas de terapéutica quirúrgica México DF: Nueva Editorial Interamericana.
    2. Bari F., Khalid M., Haresign W., Murray A., Merrell B. (2000): Effect of mating system, flushing procedure, progesterone dose and donor ewe age on the yield and quality of embryos within a MOET program in sheep. Theriogenology, 53, 727-42. Go to original source... Go to PubMed...
    3. Barry D.M., Van Niekerk C.H., Rust J., Van der Walt T. (1990): Cervical embryo collection in sheep after ripening of the cervix with prostaglandin E2 and estradiol. Theriogenology, 33, 190 pp. Go to original source...
    4. Blasco I., Folch J. (1982): Diagnóstico precoz de gestación y determinación del número de fetos por ecograf ía en ganado ovino. ITEA, 82, 22-32.
    5. Brebion P., Vallet J.C., Cognie Y., Lajous D., Baril G. (1989): Ovine embryo transfer in the lacaune breed: efficiency of a new laparoscopic technique. In: 5th Proc. Association Européenne de Transfert Embryonnaire, 134 pp.
    6. Brown G.H. (1988): The statistical comparison of reproduction rates for groups of sheep. Aust. J. Agric. Res., 39, 899-905. Cognie Y., Baril G., Poulin N., Mermillod P. (2003): Current status of embryo technologies in sheep and goat. Theriogenology, 59, 171-88. Go to original source... Go to PubMed...
    7. D'Alessandro A.G., Martemucci G., Colonna M.A., Borghese A., Terzano M.G., Bellitti A. (2001): Superovulation in ewes by a single injection of pFSH dissolved in polyvinylpyrrolidone (PVP): effects of PVP molecular weight, concentration and schedule of treatment. Anim. Reprod. Sci., 3, 255-64. Go to original source... Go to PubMed...
    8. D'Alessandro A.G., Martemucci G., Taibi L. (2005): How the FSH/LH ratio and dose numbers in the p-FSH administration treatment regimen and insemination schedule affect superovulatory response in ewes. Theriogenology, 6, 1764-1774. Go to original source... Go to PubMed...
    9. Donaldson L.E. (1990): FSHp batch variation. Theriogenology, 33, 215 pp. Go to original source...
    10. Driancourt M.A., Fry R.C. (1992): Effect of superovulation with pFSH or PMSG on growth and maturation of the ovulatory follicles in sheep. Anim. Reprod. Sci., 27, 279-292. Go to original source...
    11. Folch J., Ramon J.P., Cocero M.J., Alabart J.L., Beckers J.F. (2001): Exogenous growth hormone improves the number of transferable embryos in superovulated ewes. Theriogenology, 55, 1777-1785. Go to original source... Go to PubMed...
    12. Gandolfi F., Modina S., Brevini T.A., Galli C., Moor R. M., Lauria A. (1991): Oviduct ampullary epithelium contributes a glycoprotein to the zona pellucida, perivitelline space and blastomere membrane of sheep embryos. Eur. J. Bas. Appl. Histochem., 35, 383-392.
    13. Gonzalez-Bulnes A., Santiago-Moreno J., Cocero M.J., Lopez-Sebastian A. (2000): Effects of FSH commercial preparation and follicular status on follicular growth and superovulatory response in Spanish Merino ewes. Theriogenology, 54, 1055-1064. Go to original source... Go to PubMed...
    14. Gonzalez-Bulnes A., Garcia-Garcia R.M., Souza C.J., Santiago-Moreno J., Lopez-Sebastian A., Cocero M.J., Baird D.T. (2002): Patterns of follicular growth in superovulated sheep and influence on endocrine and ovarian response. Reprod. Domest. Anim., 6, 357-361. Go to original source... Go to PubMed...
    15. Gonzalez-Bulnes A., Baird D.T., Campbell B.K., Cocero M.J., Garcia-Garcia R.M., Inskeep E.K., Lopez-Sebastian A., McNeilly A.S., Santiago-Moreno J., Souza C. J., Veiga-Lopez A. (2004): Multiple factors affecting the efficiency of multiple ovulation and embryo transfer in sheep and goats. Reprod. Fertil. Dev., 16, 421-435. Go to original source... Go to PubMed...
    16. Halbert G.W., Dobson H., Walton J.S., Buckrell B.C. (1990): The structure of the cervical canal of the ewe. Theriogenology, 33, 977-992. Go to original source... Go to PubMed...
    17. Hunter G.L., Adams C.E., Rowson L.E.A. (1955): Interbreed ovum transfer in sheep. J. Agric. Sci. Camb., 46, 143-149. Go to original source...
    18. Khan T.H., Hastie P.M., Beck N.F., Khalid M. (2003): hCG treatment on day of mating improves embryo viability and fertility in ewe lambs. Anim. Reprod. Sci., 76, 81-89. Go to original source... Go to PubMed...
    19. Kershaw C.M., Khalid M., McGowan M.R., Ingram K., Leethongdee S., Wax G., Scaramuzzi R.J. (2005): The anatomy of the sheep cervix and its influence on the transcervical passage of an inseminating pipette into the uterine lumen. Theriogenology, 64, 1225-1235. Go to original source... Go to PubMed...
    20. López-Sebastian A., Picazo R.A., Barragan M.L., Cocero M.J., Gómez A., Cabellos B. (1992): Increase in ovulation rate after the treatment with a single injection of FSH-P diluted in propylene glycol in ewes synchronized with cloprostenol. In: Proc. 8th Association Européenne de Transfert Embryonnaire, Lyon. France, 182 pp.
    21. McKelvey W.A.C., Robinson J.J., Aitken R.P., Robertson I.S. (1986): Repeated recoveries of embryos from ewes by laparoscopy. Theriogenology, 25, 855-865. Go to original source...
    22. Niemann H., Freitag M., Elsaesser F. (1989): The role of oestrogens in early embryonic development. J. Reprod. Fertil., 38, 73-83.
    23. Oldham C.M., Lindsay D.R. (1980): Laparoscopy in the ewe: A photographic record of the ovarian activity of ewes experiencing normal or abnormal oestrus cycles. Anim. Reprod. Sci., 3, 119-124. Go to original source...
    24. Ramon J.P., Folch J. Fernandez-Arias A., Alabart J.L., Cocero M.J., Echegoyen E. (1991): La técnica de transferencia de embriones en el ganado ovino. ITEA, Extra, 11, 61-63.
    25. Torres S., Cognie Y. (1984): Superovulation and egg transfer in the ewe. Reprod. Nutr. Dev., 24, 623-631. Go to original source... Go to PubMed...
    26. Vallet J.C., Folch J., Poulin N., Cognie Y. (1989): Surgical or laparoscopic embryo transfer in sheep before or after the first cleavages. In: 5th Proc. Association Européenne de Transfert Embryonnaire, Lyon, France, 188 pp.
    27. Wilmut I., Aahworth C.J., Sales D.I. (1985): The influence of progesterone profile on embryo survival in ewes. In: Embryonic Mortality in Farm Animals. Sreenan J.M., Diskin M.G. (eds.): Martinus Nijhoff Publishers. The Hague, Netherlands, 135-141. Go to original source...
    28. Winterberger-Torres S., Sevellec C. (1987): Atlas du développement embryonaire précoce chez les ovins. Institute National de la Recherche Agronomique. Paris, France.

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