Czech J. Anim. Sci., 2007, 52(11):378-386 | DOI: 10.17221/2320-CJAS

Nitrogen degradability and intestinal digestibility of rumen undegraded protein in rapeseed, rapeseed meal and extracted rapeseed meal

P. Homolka1, J. Harazim2, J. Třináctý3
1 Institute of Animal Science, Prague-Uhříněves, Czech Republic
2 Central Institute for Supervising and Testing in Agriculture, Opava, Czech Republic
3 Research Institute for Animal Breeding, Ltd., Pohořelice, Czech Republic

In this study, nutritive values of rapeseed (R), rapeseed meal-expeller A (RM-A), rapeseed meal-expeller B (RM-B) and extracted rapeseed meal (ERM) were compared. The trials were performed using the in sacco method with three steers of the Czech Fleckvieh breed, which were fitted with a permanent ruminal cannula. Nylon bags with samples were incubated in the rumen for 2, 4, 8, 16, 24 and 48 hours. The effective degradability (ED) of crude protein (CP) was calculated at 0.08, 0.06 and 0.04 1/h of rumen particulate outflow rates (k), and the obtained ED values were 65.4, 70.8 and 77.4% for R, 86.7, 88.1 and 89.7% for RM-A, 82.2, 84.4 and 87.0% for RM-B and 56.3, 62.1 and 69.6% for ERM, respectively. The ED values significantly differed between feeds (P < 0.05) for all rumen particulate outflow rates. Disappearances of amino acids (AA) after 16 hours of incubation in the rumen of R, RM-A, RM-B and ERM were determined. In all cases, the concentrations of AA in the feeds determined after incubation in rumen were lower than in the original feeds. A mobile bag technique was used to determine intestinal digestibility. In the experiment, three dry cows fitted with permanent large ruminal cannula and the T-piece cannula in the proximal duodenum were used. The intestinal digestibilities of rumen undegraded CP (DSI) were estimated 30.0% in R, 15.4% in RM-A, 27.6% in RM-B and 65.3% in ERM. The DSI values significantly differ between the feeds (P < 0.05), except for the difference between R and RM-B.

Keywords: rapeseed; rapeseed meal; extracted rapeseed meal; ruminant; protein; amino acids; degradability; intestinal digestibility

Published: November 30, 2007  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Homolka P, Harazim J, Třináctý J. Nitrogen degradability and intestinal digestibility of rumen undegraded protein in rapeseed, rapeseed meal and extracted rapeseed meal. Czech J. Anim. Sci. 2007;52(11):378-386. doi: 10.17221/2320-CJAS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. AOAC (1984): Association of Official Analytical Chemists. Official Methods of Analysis, 14 th ed. AOAC. Washington, USA, 1141.
    2. Boila J.R., Ingalls R.J. (1992): In situ rumen digestion and escape of dry matter, nitrogen and amino acids in canola meal. Can. J. Anim. Sci., 72, 891. Go to original source...
    3. Boila J.R., Ingalls R.J. (1994): The post-ruminal digestion of dry matter, nitrogen and amino acids in wheat-based distillers dried grains and canola meal. Can. J. Anim. Sci., 49, 173-188. Go to original source...
    4. Dakowski P., Weisbjerg M.R., Hvelplund T. (1996): The effect of temperature during processing of rape seed meal on amino acid degradation in the rumen and diges­ tion in the intestine. Anim. Feed Sci. Technol., 58, 213-226. Go to original source...
    5. Demarquilly C., Andrieu J., Michalet-Doreau B., Sauvant D. (1989): Measurement of the nutritive value of feeds. In: Jarrige R. (ed.): Ruminant Nutrition. Recommended Allowances and Feed Tables. INRA Paris. 389 pp.
    6. Commission Decision 2001/25/EC (2000): Prohibiting the use of certain animal by-products in animal feed (Document no. C2000 4143). Official Journal L 006, 0016-0017.
    7. Frydrych Z. (1992): Intestinal digestibility of rumen unde­ graded protein of various feeds as estimated by the mobile bag technique. Anim. Feed Sci. Technol., 37, 161-172. Go to original source...
    8. Gőpfert E., Trčková M., Dvořák R. (2006) The use of treated rape cake in a calf starter diet. Czech J. Anim. Sci., 51, 491-501. Go to original source...
    9. Harazim J., Třináctý J., Homolka P. (2002): Degradability and intestinal digestibility of crude protein and amino acids of extracted rapeseed meal. Czech J. Anim. Sci., 47, 50-56.
    10. Homolka P., Tománková O., Komprda T., Frydrych Z. (1996): PDI protein evaluation system of feeds for ruminants. ÚZPI, Prague, 4, 1-33. (in Czech)
    11. Kendall M.E., Ingalls R.J., Boila J.R. (1991): Variability in the rumen degradability and post-ruminal digestion of the dry matter, nitrogen and amino acids of canola meal. Can. J. Anim. Sci., 71, 739-754. Go to original source...
    12. Komprda T., Dvořák R., Fialová M., Šustová A., Pechová A. (2005): Fatty acid in milk of dairy cows on a diet with high fat content derived from rapeseed. Czech J. Anim. Sci., 50, 311-319. Go to original source...
    13. Kudrna V., Marounek M. (2006): The influence of feeding rapeseed cake and extruded soyabean on the performance of lactating cows and the fatty acid pattern of milk. J. Anim. Feed Sci., 15, 361-370. Go to original source...
    14. Masoero F., Fiorentini L., Rossi F., Piva A. (1994): Determi­ nation of nitrogen intestinal digestibility in ruminants. Anim. Feed Sci. Technol., 48, 253-263. Go to original source...
    15. Moshtaghi Nia A.S., Ingalls R.J. (1995): Influence of moist heat treatment on ruminal and intestinal disappearance of amino acids from canola meal. J. Dairy Sci., 78, 1552-1560. Go to original source... Go to PubMed...
    16. Ørskov E.R. (1992): Protein nutrition in ruminants. 2nd ed., Academic Press, London, 175 pp.
    17. Ørskov E.R., McDonald I. (1979): The estimation of pro­ tein degradability in the rumen from incubation measure­ments weighted according to rate of passage. J. Agric. Sci., 92, 499-503. Go to original source...
    18. Rooke A.J. (1985): The nutritive values of feed proteins and feed proteins residues resistant to degradation by rumen micro-organism. J. Sci. Food Agric., 36, 629-637. Go to original source...
    19. Sauvant D., Perez J.M., Tran G. (2004): Tables of composition and nutritional value of feed materials. Wageningen Academic Publisher. Netherlands and INRA Paris, France. 304 pp. Go to original source...
    20. SPSS (2000): SPSS 13.0 for Windows. Apache Software Foundation, SPSS Inc.
    21. Strusinska D., Minakowski D., Pysera B. Kaliniewicz J. (2006): Effects of fat-protein supplementation of diets for cows in early lactation on milk yield and composition. Czech J. Anim. Sci., 51, 196-204. Go to original source...
    22. Třináctý J., Šimek M., Komprda T. (1996): The influence of a nylon bag carrier on alfalfa crude protein degradability. Anim. Feed Sci. Technol., 57, 129-137. Go to original source...
    23. Vencl B., Frydrych Z., Krása A., Pospíšil R., Pozdíšek J., Sommer A., Šimek M., Zeman L. (1991): The new sys­ tems of feed evaluation for cattle. Sborník AZV ČSFR, 148, 134 pp.
    24. Vérité E.R., Michalet-Doreau B., Chapoutot P., Peyrand J.L., Poncet C. (1988): Révision du système des protéines digestibles dans l'intestin (PDI). Bull. Tech. CRZV Theix, INRA, 70, 19-34.
    25. Zedník J. (1999): Monitoring for production of forage mixtures. ÚKZÚZ, Brno. 56 pp. (in Czech)
    26. Zeman L. a kol. (1995): Feedstuffs tables. VÚVZ, Pohořelice. 465 pp. (in Czech)

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content