Czech J. Anim. Sci., 2007, 52(1):1-6 | DOI: 10.17221/2329-CJAS

Study of an association between SNP 775C>T within the bovine ITBG2 gene and milk performance traits in Black and White cows

U. Czarnik1, M. Galiński1, Ch.S. Pareek1, T. Zabolewicz1, Z. Wielgosz-Groth2
1 Department of Animal Genetics, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland
2 Department of Cattle Breeding, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland

The exclusive pre-selective effect of BLAD carriers reproducing in the exposed Holstein-Friesian cattle population prompted to search for the candidate gene variants of high quality of milk performance traits within the bovine ITBG2 gene or loci linked with bovine ITBG2 gene. Theoretical considerations indicated that the &Idquo;silent mutation" C→T localized at the position of 775 bp of the gene encoding the CD18 subunit might be a potential QTL marker of high milk productivity. An association study between the polymorphism of SNP 775C>T, and the diversification of milk performance traits was carried out on the progeny of four bulls with genotypes BL/TL and 775C/T and one bull with genotypes TL/TL and 775C/T. The results documented statistically significant differences in the protein content percentage of milk in two half-sib families of bulls with BL/TL and 775C/T genotype and one half-sib family of bull with TL/TL and 775C/T genotype. It was further concluded that the polymorphism of SNP 775C>T was found to be a more efficient QTL marker than that of D128G, since in all the analysed milk performance traits for half-sib families higher values of the F coefficient were obtained for the SNP 775C>T mutation in comparison with D128G.

Keywords: BLAD carriers; point mutation; lethal genes; milk performance traits; QTL marker

Published: January 31, 2007  Show citation

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Czarnik U, Galiński M, Pareek CS, Zabolewicz T, Wielgosz-Groth Z. Study of an association between SNP 775C>T within the bovine ITBG2 gene and milk performance traits in Black and White cows. Czech J. Anim. Sci. 2007;52(1):1-6. doi: 10.17221/2329-CJAS.
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    References

    1. Cox E., Mast J., MacHugh N., Schwenger B., Goddeeris B.M. (1997): Expression of b2 integrins on blood leukocytes of cows with or without bovine leukocyte adhesion deficiency. Vet. Immunol. Immunopath., 58, 249-263. Go to original source... Go to PubMed...
    2. Czarnik U. (2000): Breeding and genetic population aspects of the occurrence of BLAD (Bovine Leukocyte Adhesion Deficiency) in Black-and-White cattle. Dissertation and Monographs, 32, 1-44. (In Polish) 
    3. Czarnik U., Zabolewicz T., Galiński M., Pareek C.S., Walawski K. (2004): Silent point mutation polymorphism of the bovine CD18 encoding gene. J. Appl. Genet., 45, 73-76. Go to PubMed...
    4. Doerchuk C.M., Winn R.U., Harlan J.M. (1989): Mechanisms of neutrophil emigration. In: Springer T.A., Anderson D.C., Rosenthal A.S. et al. (eds.): Structure and function of molecules involved in leukocyte adhesion. Springer-Verlag, New York, 87-94. Go to original source...
    5. Don R.H., Cox P.T., Wainwright B.T., Beker K., Mattick J.S. (1991): Touchdown PCR to circumvent spurious priming during gene amplification. Nucl. Acids Res., 14, 4008. Go to original source... Go to PubMed...
    6. Fett T., Zecchinon L., Baise E., Desmecht D. (2004): The bovine (Bos taurus) CD11a-encoding cDNA: molecular cloning, characterization and comparison with the human and murine glycoproteins. Gene, 325, 97- 101. Go to original source... Go to PubMed...
    7. Georges M., Nielsen D., Mackinnon M., Mishra A., Okimoto R., Pasquino A.T., Sargeant L.S., Sorensen A., Steele M.R., Zhao X., Womack J.E., Hoeschele I. (1995): Mapping quantitative trait loci controlling milk production in dairy cattle by exploiting progeny testing. Genetics, 139, 907-920. Go to original source... Go to PubMed...
    8. Hynes R.O. (1992): Integrins, versality, modulation, and signalling in cell adhesion. Cell, 69, 11-25. Go to original source... Go to PubMed...
    9. Janosa A., Baranyai B., Dohy J. (1999): Comparison of milk production of the progeny of BLAD-carrier and healthy Holstein bulls in Hungary. Acta Vet. Hung., 47, 283-289. Go to original source... Go to PubMed...
    10. Kijas J.M.H., Bauer T.R., Gäfvert S., Marklund S., Trowald-Wigh G., Johannisson A., Hedhammar Å., Binns M., Juneja R.K., Hickstein D.D., Andersson L. (1999): A missence mutation in the b-2 integrin gene (ITGB2) causes canine leukocyte adhesion deficiency. Genomics, 61, 101-107. Go to original source... Go to PubMed...
    11. Lubieniecki K., Grzybowski G., £ukaszewicz M., Lubieniecka J. (1999): Association between the presence of allele BL in the genome of dairy cows and their productivity. Anim. Sci. Pap. Rep., 17, 189-194.
    12. Mosig M.O., Lipkin E., Khutoreskaya G., Tchourzyna E., Soller M., Friedmann A. (2001): A whole genome scan for quantitative trait loci affecting milk protein percentage in Israeli-Holstein cattle, by means milk DNA pooling in daughter design, using an adjusted false discovery rate criterion. Genetics, 157, 1683-1698. Go to original source... Go to PubMed...
    13. Nadesalingam J., Plante Y., Gibson J.P. (2001): Detection of QTL for milk production on Chromosomes 1 and 6 of Holstein cattle. Mam. Genome, 12, 27-31. Go to original source... Go to PubMed...
    14. Shuster D.E., Bosworth B.T., Kehrli M.E. (1992a): Sequence of the bovine CD18-encoding cDNA; comparison with human and murine glycoproteins. Gene, 114, 267-271. Go to original source... Go to PubMed...
    15. Shuster D.E., Kehrli M.E., Ackermann M.R., Gilbert R.O. (1992b): Identification and prevalence of a genetic defect that causes leukocyte adhesion deficiency in Holstein cattle. Proc. Natl. Acad. Sci., 89, 9225-9229. Go to original source... Go to PubMed...
    16. Taralik K. (1998): Association between BLAD carrier and non BLAD carrier Holstein-Friesian bulls and the production of their daughters. Arch. Tierz., 41, 339-344.
    17. Taylor J.F., Eggen A., Aleyasin A., Armitage S.M., Barendse W., Beever J.E., Bishop M.D., Brenneman R.A., Burns B.M., Davis S.K., Elo K., Harlizius B., Kappes S.M., Keele J.W., Kemp S.J., Kirkpatrick B.W., Lewin H.A., Ma R.Z., McGraw R.A., Pomp D., Stone R.T., Sugimoto Y., Teale A.J., Vaiman D., Vilkki J., Williams J.L., Eh C.C., Zanotti M.C. (1998): Report of the first workshop on the genetic map of bovine chromosome 1. Anim. Genet., 29, 228-235. Go to original source... Go to PubMed...
    18. Zecchinon L., Fett T., Baise E., Desmecht D. (2004): Molecular cloning and characterization of the CD18 partner in ovine (Ovis aries) b2-integrins. Gene, 334, 47-52. Go to original source... Go to PubMed...

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