Czech J. Anim. Sci., 2004, 49(12):542-548 | DOI: 10.17221/4342-CJAS

Effects of supplemental phytase on performance and tibia ash of broilers fed different cereals based diets

J. Pintar, B. Homen, K. Gazić, D. Grbeša, M. Sikirić, T. Černy
1 , B. H 1, K. G 1, D. G 1, M. S 1,2, T. Č 1 1Department of Animal Nutrition, Faculty of Agriculture, University of Zagreb, Croatia
2 Laboratory of Radiochemistry, Ruđer Bošković Institute, Zagreb, Croatia

A 21-day experiment with day-old broilers was conducted in order to assess the effect of phytase supplementation to different cereals-soybean meal based diets on broiler performance and tibia ash. Diets were formulated to contain 4 different cereals (maize, wheat, triticale and barley), 2 levels of dietary calcium (0.6 and 1.0%) and 3 levels of supplemental phytase (0, 500 and 1 000 PU/kg). Supplemented phytase had beneficial effects on broiler performance. It significantly increased body weight gain (P < 0.0003) and feed consumption (P < 0.0361) by 6 and 7% in comparison with the control groups, respectively. No influence on feed conversion ratio and tibia ash was detected. Both body weight gain and feed intake were also significantly influenced by different cereals (P < 0.0001 and 0.0348, respectively). The increasing dietary calcium level resulted in a significant increase in body weight gain (P < 0.0024) and tibia ash (P < 0.0016). Effects of 500 and 1 000 PU/kg were not statistically different between themselves.

Keywords: phytase; maize; wheat; triticale; barley; body weight gain; feed consumption; feed conversion ratio; tibia ash

Published: December 31, 2004  Show citation

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Pintar J, Homen B, Gazić K, Grbeša D, Sikirić M, Černy T. Effects of supplemental phytase on performance and tibia ash of broilers fed different cereals based diets. Czech J. Anim. Sci. 2004;49(12):542-548. doi: 10.17221/4342-CJAS.
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    References

    1. Broz J., Oldale P., Perrin-Voltz A.H., Rychen G., Schulze J., Simoes Nunes C. (1994): Effect of supplemental phytase on performance and phosphorus utilization in broiler chickens fed a low phosphorus diet without addition of inorganic phosphates. Brit. Poultry Sci., 35, 273-280. Go to original source... Go to PubMed...
    2. Edens F.W., Parkhurst C.R., Havenstein G.B. (1999): Enzyme phytase reduces phosphorus and nitrogen excretion by caged broilers and by broilers in conventional housing. Under the Microscope, Focal Points for the New Millennium, Biotechnology in the Feed Industry, Proceeding of Alltech's 15th Annual Symposium, 491-509.
    3. Edwards H.M., Jr. (1993): Dietary 1,25-Dihydroxycholecalciferol supplementation Increases Natural Phytate Phosphorus Utilization in Chickens. J. Nutr., 123, 567-577. Go to original source... Go to PubMed...
    4. Harter-Dennis J.M., Sterling K. (1999): Evaluation of phytase in corn based diets in male broilers from 21-42 days of age. Dept. of Agriculture, University of Maryland Eastern Shore, Princess Anne, MD. SPSS Abstracts S169.
    5. Huyghebaert G. (1996): The response of broiler chicks to phase feeding for P, Ca and phytase. Arch. Geflügelkde, 60, 132-141.
    6. Huyghebaert G. (1997): Effect of dietary calcium, phosphorus, Ca/P-ratio and phytase on zootechnical performances and mineralization in broiler chicks. Arch. Geflügelkde, 61, 53-61.
    7. ISO 5498 (1999): Agricultural food products - Determination of crude fibre content - General method.
    8. ISO 5983 (1997): Animal feeding stuffs - Determination of nitrogen content and calculation of crude protein content - Kjeldahl method.
    9. ISO 5984 (2002): Animal feeding stuffs - Determination of crude ash.
    10. ISO 6490-1 (1985): Animal feeding stuffs - Determination of calcium content - Part 1: Titrimetric method.
    11. ISO 6491 (1998): Animal feeding stuffs - Determination of phosphorus content - Spectrometric method.
    12. ISO 6492 (1999): Animal feeding stuffs - Determination of fat content.
    13. ISO 6496 (1999): Animal feeding stuffs - Determination of moisture and other volatile ma er content.
    14. Kiiskinen T., Piironen J., Hakonen T. (1994): Effects of supplemental microbial phytase on performance of broiler chickens. Agric. Sci. Finland, 3, 457-465. Go to original source...
    15. Knuckles B.E., Betschart A.A. (1987): Effect of phytate and other myo-inositol esters on α-amylase digestion of starch. J. Food Sci., 52, 719-721. Go to original source...
    16. Perney K.M., Cantor A.H., Straw M.L., Herkelman K.L. (1993): The effect of dietary phytase on growth performance and phosphorus utilization of broiler chickens. Poultry Sci., 72, 2106-2114. Go to original source... Go to PubMed...
    17. Qian H., Kornegay E.T., Denbow D.M. (1996a): Phosphorus equivalence of microbial phytase in turkey diets as influenced by calcium to phosphorus ratios and phosphorus levels. Poultry Sci., 75, 69-81. Go to original source... Go to PubMed...
    18. Qian H., Veit H.P., Kornegay E.T., Ravindran V., Denbow D.M. (1996b): Effects of supplemental phytase and phosphorus on histological and other tibial bone characteristics and performances of broilers fed semi-purified diets. Poultry Sci., 75, 618-626. Go to original source... Go to PubMed...
    19. Ravindran V., Bryden W.L., Kornegay E.T. (1995): Phytates: occurrence, and implications in poultry nutrition. Poultry Avian Biol. Rev., 6, 125-143.
    20. Ravindran V., Cabahug S., Ravindran G., Bryden W.L. (1999): Influence of microbial phytase on apparent ileal amino acid digestibility of feedstuffs for broilers. Poultry Sci., 78, 699-706. Go to original source... Go to PubMed...
    21. SAS Institute Inc. (1999): The SAS System for Windows. Release 8.00.
    22. Sebastian S., Touchburn S.P., Chavez E.R., Lague P.C. (1996a): Efficacy of supplemental microbial phytase at different dietary calcium levels on growth performance and mineral utilisation of broiler chickens. Poultry Sci., 75, 1516-1523. Go to original source... Go to PubMed...
    23. Sebastian S., Touchburn S.P., Chavez E.R., Lague P.C. (1996b): The effects of supplemental microbial phytase on the performance and utilisation of dietary calcium, phosphorus, copper and zinc in broiler chickens fed corn-soybean diets. Poultry Sci., 75, 729-736. Go to original source... Go to PubMed...
    24. Shirley R.B., Edwards H.M., Jr. (2003): Graded levels of phytase past industry standards improves broiler performance. Poultry Sci., 82, 671-680. Go to original source... Go to PubMed...
    25. Simons P.C.M., Versteegh H.A.J., Jongbloed A.W., Kemme P.A., Slump P., Bos K.D., Wolters M.G.E., Beudeker R.F., Verschoor G.J. (1990): Improvement of phosphorus availability by microbial phytase in broilers and pigs. Brit. J. Nutr., 64, 525-540. Go to original source... Go to PubMed...
    26. Smith A.K., Rackis J.J. (1957): Phytin elimination in soybean protein. J. Am. Chem. Soc., 79, 633-637. Go to original source...
    27. Sohail S.S., Roland D.A. (1999): Influence of supplemental phytase on performance of broilers four to six weeks of age. Poultry Sci., 78, 550-556. Go to original source... Go to PubMed...
    28. Sooncharernying S., Edwards H.M., Jr. (1993): Phytate contents of excreta and phytate retention in the gastrointestinal tract of young chickens. Poultry Sci., 72, 1906-1916. Go to original source...
    29. Um J.S., Paik I.K. (1999): Effects of microbial phytase supplementation on egg production, eggshell quality, and mineral retention of laying hens fed different levels of phosphorus. Poultry Sci., 78, 75-79. Go to original source... Go to PubMed...
    30. Underwood E.J. (1981): The Mineral Nutrition of Livestock. 2nd Ed. Commonwealth Agricultural Bureau X.
    31. Yi Z., Kornegay E.T., Ravindran V., Denbow D.M. (1996): Improving phytate phosphorus availability in corn and soybean meal for broilers using microbial phytase and calculation of phosphorus equivalency values for phytase. Poultry Sci., 75, 240-249. Go to original source... Go to PubMed...
    32. Windisch W., Kirchgessner M. (1996): Effect of microbial phytase supplementation on performance data and metabolism of phosphorus, calcium and nitrogen at different levels of calcium supply in broilers. Arch. Geflügelkde, 60, 42-47.
    33. Zyla K., Korelski J., Swiatkiewicz S., Wikiera A., Kujawski M., Piironen J., Ledoux D.R. (2000): Effect of phosphorylitic and cell wall-degrading enzymes on the performance of growing broilers fed wheat-based diets containing different calcium levels. Poultry Sci., 79, 66-76. Go to original source... Go to PubMed...

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