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The genomic structure of extant cattle populations can contribute to the reconstruction of the history of particular breeds or their subpopulations. Genome-wide population resequencing of extant populations of Czech Red Pied (CRP) cattle, its conserved nucleus herd, and Czech Red (CR) cattle detected a T106C polymorphism in mitochondrial DNA shared by the conserved CRP herd with a geographical belt of Anatolian, Illyrian and Eastern Alpine breeds. On the other hand, this SNP is practically absent in the historical cattle breeds associated with Northern Germany, including German Black Pied cattle, Holstein-Friesian and German Red Mountain cattle (GRM). Correspondingly, this indicator SNP was absent in CR cattle, which, like the GRM, belongs to the group of mountain red cattle breeds. It seems that the precursory cattle population in Central Europe was influenced by the germplasm from the Balkans-related group of breeds, thanks to the political and commercial influence of the Northern Italian, Austrian and Hungarian regions. In addition, the presence of this polymorphism in some European aurochs (Bos primigenius) bone remains suggests possible introgression from local aurochs populations. Alternatively, the T106C presence in yaks and in neighbouring cattle breeds in Northern India, China and Korea raises the possibility of origin of this polymorphism from yak populations. The spread of the T106C mutation in alpine regions is consistent with the known role of this mtDNA region in adaptation to the reduced oxygen pressure.

Our study investigated the genetic variability of specific foot and claw disorders, namely dermatitis digitalis, sole ulcer, and three broader categories (infectious diseases, claw horn lesions, and general claw disorder) in Czech Holstein cows. We also examined their genetic relationships with clinical mastitis and key fertility traits. Using both linear and threshold animal models, we concluded that heritability estimates for foot and claw disorders were consistently higher when employing the threshold model. A significant finding was the genetic correlation between sole ulcer and dermatitis digitalis, which ranged from 0.21 (calving to 305 days in milk) to 0.53 (calving to 90 days in milk). Furthermore, we observed stronger genetic correlations between clinical mastitis and overall foot and claw disorders, claw horn lesions, and infectious diseases during early lactation (calving to 90 days in milk) compared to the entire lactation period. Within this early lactation phase, the strongest genetic correlation was identified between clinical mastitis and claw horn lesions. Interestingly, our research uncovered a unique genetic link between days open and dermatitis digitalis, suggesting that a genetic predisposition to a higher occurrence of dermatitis digitalis may coincide with shorter days open. These results underscore the importance of implementing distinct genetic selection strategies for infectious claw diseases and claw horn lesions in dairy cows. This distinction is crucial due to their differing genetic correlations with other economically important traits, ultimately contributing to a better understanding of claw health genetics and its application in breeding programs.

The aim of the study was to verify whether differences in intramuscular fat quality, evaluation based on fatty acid profile and ratios, occur between cattle genotypes – the combined performance cattle breed Czech Fleckvieh and the beef breed Aberdeen Angus – under identical rearing conditions (same pasture location and nutritional management) with extensive grazing. The results of observation show the difference in the quality of intramuscular fat of bulls with combined performance and bulls with meat performance in extensive pastoral farming. Breed differences were reflected by a statistically significantly higher mean intramuscular fat (P ≤ 0.05) in the beef cattle breed (149 ± 60.1 g/kg dry matter) compared to the combined performance breed (120 ± 51.8 g/kg dry matter). A higher saturated fatty acid (SFA) value of 53.4 ± 22.2 g/kg dry matter was observed in the beef cattle breed compared to SFA 40.8 ± 18.9 g/kg dry matter (P ≤ 0.05) in the combined performance cattle breed; a higher monounsaturated fatty acid (MUFA) value of 43.8 ± 26.3 g/kg dry matter compared to a MUFA value of 42.3 ± 20.8 g/kg dry matter (P > 0.05); and a higher value of polyunsaturated fatty acids n-3 (PUFA n-3) 0.76 ± 0.33 g/kg dry matter (P ≤ 0.05) compared to PUFA n-3 0.48 ± 0.21 g/kg dry matter in combined performance cattle breed. A higher value of polyunsaturated fatty acids n-6 (PUFA n-6) 3.66 ± 1.39 g/kg dry matter was found in the combined performance cattle breed compared to the meat cattle breed with PUFA n-6 3.54 ± 0.96 g/kg dry matter (P > 0.05). A more favourable n-6/n-3 PUFA ratio was observed in the Aberdeen Angus (P ≤ 0.05). The results suggest that the different genotypes of the bull breeds studied, reared on extensively used pasture, may influence the amount of intramuscular fat and its quality.

In addition to their practical importance as a medicinal plant, animal feed and a source of materials for the textile and construction industry, industrial varieties of Cannabis sativa L. (hemp in a wider sense) provide an alternative for controlling infectious diseases in livestock. Despite the genetic divergence between two primary groups of cannabis, i.e. medicinal cannabis and technical hemp, hemp plants also produce a wide spectrum of secondary metabolites. These include the main classes of cannabinoids and terpenoids, as well as representatives of flavonoids, stilbenoids, steroids, alkaloids, spiroindans, dihydrophenanthrenes, and lignanamides. Many of them exhibit antibiotic activity which can substitute or complement the use of traditional antibiotics in animal husbandry. For example, the cannabinoid fraction exhibits activity against the Gram-positive bacteria and some fungi. While the activity against Gram-negative bacteria is not characteristic of cannabinoids, these pathogens can still be affected by hemp terpenoids and flavonoids. The synergy among the secondary metabolite fractions or between the hemp metabolites and traditional antibiotics is also a favourable factor. The search for alternatives to traditional antibiotics is further driven by the increasing prevalence of genetically determined antibiotic resistance among veterinary pathogens, which poses the additional risk of transferring resistance traits to the human pathogens. The content of antibiotically active compounds in hemp can be enhanced through selection among existing genotypes, targeted breeding, cultivation conditions, and even by specific elicitation of secondary metabolites with the natural antibiotic function in the disease resistance of the plant. The switch to hemp metabolites is also supported by their compatibility as natural components of plant-based animal feed, and by favourable economic considerations.

Polyunsaturated fatty acids (PUFAs), including omega-3, are known to yield health benefits to mammals, including bulls and human beings. This meta-analysis was done to determine the influence of omega-3 fatty acids (OMFA) on bull semen quality. The research question formulated for this meta-analysis was centred on the Population-Intervention-Comparison-Outcome (PICO) framework. To determine the influence of omega-3 fatty acids on semen quality, a meta-analysis was conducted using the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines. The different effects were analysed using a random effects model. The variations among the included studies were tested using heterogeneity (I²). Semen volume (MD = 0.28, 95% CI: 0.13; 0.44), sperm concentration (MD = 71.12, 95% CI: 15.97; 126.26), live sperm (MD = 11.93; 95% CI: 7.30; 16.55), sperm motility (MD = 9.24, 95% CI: 7.39; 11.09) and plasma membrane integrity (MD = 11.60, 95% CI: 6.30; 16.89) increased dramatically in the omega-3 fatty acid-supplemented group. Notably, the progressive motility (MD = 1.19, 95% CI: –3.24; 5.61) was the only sperm parameter showing a non-significant influence when omega-3 fatty acids were supplemented. It was then concluded that the omega-3 fatty acid supplementation can be used to improve most of the semen parameters in bulls.

The aim of this study was to identify and quantify the relationships between the nutrient content and the DM (dry matter) content of various maize hybrids (Zea mays L.) in the dynamics of vegetative maturity and various soil-climatic growing conditions. Over the course of 7 growing seasons (years), a set of 1 972 samples of whole silage maize plants consisting of 206 different hybrids grown in two contrasting regions (lowland and foothill areas) was analysed. The focus was on DM content and the content of key energy nutrients (WSC – water soluble carbohydrates, starch, NDF – neutral detergent fibre) and their interactions. Results show that the transformation of WSC into starch has four key points: (i) it begins at a DM content of 150 g/kg; (ii) it peaks at a DM content of approximately 235 g/kg; (iii) it begins to decrease significantly from a DM content of 300 g/kg; and (iv) it practically stops rapidly after exceeding a DM content of 350 g/kg. In the dynamics of vegetative development of maize plants, the DM content is very closely related to the WSC content (R² = 0.728) and the starch content (R² = 0.873). With the gradual increase in vegetative maturity and DM content in maize plants, the transformation of WSC into starch dynamically increases. These characteristics, with small deviations, were also confirmed at different levels of evaluation (all analyses, regions, seasons and individual hybrids). These results show that a DM content of 300 g/kg to 350 g/kg can be considered the optimal harvesting window for maize ensiling and the optimal phase of silage maturity for whole maize plants, because once the DM content exceeds 350 g/kg; the transformation of WSC into starch stops and the drying phase of plants begins.

Innate immunity is the organism’s first line of defence, and the receptors of immune cells play a key role. Interleukin 1 receptor-associated kinase 1 and 4 (IRAK1 and IRAK4) are necessary for signal transmission from Toll-like receptors, which are the most important receptors of immune cells. The main objective of this study was to investigate the gene polymorphisms of IRAK1 and IRAK4 in Czech Fleckvieh cattle (CF) and to compare their diversity with other cattle breeds as there is still little information about these genes. PacBio Technology of next-generation sequencing (NGS) was used to detect genetic variability in 164 CF bulls. The results revealed 17 single nucleotide variants (SNVs) in the IRAK1 gene and 12 SNVs and three deletions in the IRAK4 gene. Three methods were then used to determine allelic variability: Sanger sequencing, the primer extension method and Illumina DNA BeadChip microarray in cooperation with the Czech Moravian Breeder’s Corporation. Sanger sequencing enabled analysis of the population variability in single nucleotide polymorphisms (SNPs) rs210710958 and rs48726521 of IRAK1 in 164 CF bulls. SNaPshot genotyping was employed to determine the allelic frequencies of SNP rs211379365 and a novel polymorphism in IRAK1, and rs380202447 in the IRAK4 gene. DNA microarray method, which was applied to 74 CF dairy cows, revealed the allelic frequency of IRAK1 rs110533802 including the frequency 0.07 of mutant allele T. Additional microarray data allowed the comparison of the allelic frequencies among the Czech and Hungarian populations of Holstein dairy cows and beef breed populations. The results show that the functionally significant diversity in the IRAK1 and IRAK4 genes in the studied CF population is rather limited. The higher frequency 0.4 of the T mutant allele of SNP rs110533802 of IRAK1 in Holstein cattle could be the consequence of breeding for milk performance.

The gilt development level is a critical factor for profitability in the pig industry. To evaluate the impact of different gilt development levels on the age structure and production efficiency of female herds we used the performance data of a pig farm that was simulated over a ten-year period, with current production statistics from the Chinese pig industry as a basis for the model. In a herd of 600 breeding sows, we compared age structure, number born alive (NBA), longevity and sow lifetime productivity in three scenarios with gilt culling rates of 10%, 20%, and 30%. The results show that the gilt development has no significant effects on the age structure. The gilt development had no significant effects either on sow reproductive performance or production efficiency. However, the annual replacement rate, longevity, and sow lifetime productivity varied significantly in the three levels of gilt development (P < 0.01). We conclude that the gilt development is closely related to herd turnover and reproductive performance, and ultimately affects the cost of pig production. Gilt development is, therefore, an extremely important factor in an integrated pig production system.

Based on a data set comprising 2879 animals and 17 nuclear microsatellite DNA markers, we propose the most comprehensive in-depth study mapping the genetic structure and specifying the assignment success rates in horse breeds at the Czech population scale. The STRUCTURE program was used to perform systematic Bayesian clustering via the Markov chain Monte Carlo estimation, enabling us to explain the population stratification and to identify genetic structure patterns within breeds worldwide. In total, 182 different alleles were found over all the populations and markers, with the mean number of 10.7 alleles per locus. The expected heterozygosity ranged from 0.459 (Friesian) to 0.775 (Welsh Part Bred), and the average level reached 0.721. The average observed heterozygosity corresponded to 0.709, with the highest value detected in the Czech Sport Pony (0.775). The largest number of private alleles was found in Equus przewalskii. The population inbreeding coefficient F_IS ranged from -0.08 in the Merens to 0.14 in the Belgian Warmblood. The total within-population inbreeding coefficient was estimated to be moderate. As expected, very large genetic differentiation and small gene flow were established between the Friesian and Equus przewalskii (F_ST = 0.37, Nm = 0.43). Zero F_ST values indicated no differences between the Czech Warmblood-Slovak Warmblood and the Czech Warmblood-Bavarian Warmblood. A high level of breeding and connectivity was revealed between the Slovak Warmblood-Bavarian Warmblood, Dutch Warmblood-Oldenburg Horse, Bavarian Warmblood-Dutch Warmblood, and Bavarian Warmblood-Oldenburg Horse. The breeds' contribution equalled about 6% of the total genetic variability. The overall proportion of individuals correctly assigned to a population corresponded to 82.4%. The posterior Bayesian approach revealed a hierarchical dynamic genetic structure in four clusters (hot-blooded, warm-blooded, cold-blooded, and pony). While most of the populations were genetically distinct from each other and well-arranged with solid breed structures, some of the entire sets showed signs of admixture and/or fragmentation.

Dairy farming is deeply affected by climate change, especially by rising temperatures and heat waves, poorer availability of quality food and water, and the spread of new diseases and pests outside their original ecological niche. Their impact can be mitigated not only by changes in technologies, management and treatment, but also by breeding and selection of more resilient cows. General resilience encompasses the animal's capacity to cope with environmental, social and disease challenges. It is described as the capacity of the animal to be minimally affected by a disturbance or to rapidly return to the physiological, behavioural, cognitive, health, affective and production states that pertained before exposure to a disturbance. As disturbances can be of different natures, general resilience is a composite trait consisting of different resilience types according to the nature of the disturbance. Resilience can be quantified through time series data that capture fluctuations in the daily performance. Recent studies have worked with deviations in the daily milk yield and daily live weight from optimal performance or have focused on the assessment of the daily activity in terms of the daily step count. To observe the duration and magnitude of the response to perturbance, two indicators were suggested: the autocorrelation (r_auto) and the natural logarithm of deviations (LnVar). Based on the daily milk yield deviations, both indicators have shown sufficient genetic variabilities with the estimated heritability ~0.1 for r_auto and ~0.2 for LnVar. Low values of both indicators were genetically related to better udder health, better hoof health, better longevity, better fertility, higher body condition score, less ketosis but also lower milk yield level. The selection for improved resilience could benefit from the use of genomic information as several genes and biological pathways associated with disease resilience and resilience to heat stress have already been identified. The presented results suggest that the integration of resilience into the cattle breeding programmes would improve the capacity of the dairy industry to cope with global climate change.

Seasonal heat stress and metabolic disorders during mid-lactation are the main factors limiting production in Holstein cows, and several proteins and molecules involved in metabolic pathways are altered in response to stress. We investigated the effects of heat stress on the milk yield, milk composition, serum oxidative status, and metabolites in Holstein cows during mid-lactation to identify biomarkers associated with heat stress in serum and milk. Holstein cows with similar body condition scores (3.0 ± 0.25), parity (2.5 ± 0.5), and lactation days (115 ± 5 days) were selected in August (heat stress, HS, n = 20) 2017 and March 2018 (non-heat stress, NHS, n = 20). Milk yield was recorded daily and serum was collected on days 1, 31, and 61. Serum and milk metabolites were analysed by gas chromatography-mass spectrometry on day 1. The results showed a significantly lower average daily milk yield in the HS group than in the NHS group (P < 0.05). The milk compositions of fat (%), lactoprotein, lactose yield, and milk solid-not-fat in the HS group were significantly lower than in the NHS group on days 1, 31, and 61 (P < 0.05). The levels of  malondialdehyde were higher, whereas those of superoxide dismutase and glutathione peroxidase were lower in the serum of the HS group (P < 0.05) than that of the NHS group. The serum concentrations of d-glucose, 9,12-octadecadienoic acid, and d-lactose were significantly higher in  the NHS group than in the HS group (P < 0.05). The concentrations of lactic acid and milk urea nitrogen in the NHS group were lower than those in the HS group (P < 0.05). The present data suggest that metabolic biomarkers are closely associated with heat stress in the serum and milk, which provides a basis for evaluating indicators of heat stress occurrence in mid-lactation cows.

In the experimental section of the barn (which made up 25% of the barn), 103 cubicles in free stall barn were selected in three rows, housing 98 dairy cows of Czech Fleckvieh cattle. Monitoring was done for one calendar year, once a week. From this period, 16 days with temperatures from 0.5–15.0 °C were selected for quantification. Observations were continuously recorded at 10:00 am (one hour after the coming of the last cow from the milking parlour), at hourly intervals until 7:00 pm. Ten observations were carried out every day of the monitoring period. In total, 15 680 individual observations were recorded. The microclimatic parameters (temperature, relative humidity) were monitored within the barns continuously at 15-minute intervals using three data logger sensors. Changes in the proportion of lying to standing dairy cows were observed during the day. A steady trend of a reduced ratio of lying dairy cows during the day (from 70% to 40%) in favour of standing dairy cows was observed. The optimal value of the cow comfort index – CCI (85%) was not reached in any monitored part of the day. The number of dairy cows lying on their left sides increased from 50.4% to 56%, especially after feeding (at 4:00 pm). Differences were found in the proportion of lying and standing cows (P < 0.05) at an optimal microclimate during the day. A significant preference for the left side when lying down was also found.

The aim of this study was to evaluate population parameters and to describe the genetic variability in the Lipizzan horse populations from stud farms in Bosnia and Herzegovina (BH) and Serbia (SRB), reported for the first time in the literature. Pedigree data were collected from the national studbooks. Pedigree data for 2 521 horses were used in the analysis, for a reference population covering live animals in the current population. The average generation interval was 10.78 ± 0.36 (BH) and 12.12 ± 0.59 (SRB) years. The equivalent complete generations ranged from 5.49 (BH) to 14.86 (SRB). The average inbreeding values were 3.96 (BH) and 2.12 (SRB), whereas the average relatedness values were 9.24 (BH) and 4.13 (SRB). Values for f_e, f_a, f_g and the f_e/f_a ratio were 21, 12, 6.031 and 1.75 in BH Lipizzan horses, and 79, 20, 9.766 and 3.95 in SRB Lipizzan horses. The number of ancestors explaining 50% of genetic diversity in the BH and SRB reference populations was five and eight, respectively. The effective population sizes obtained from regression on the birth dates were 43.67 (BH) and 211.78 (SRB). The investigated genetic variability parameters were higher in the SRB Lipizzan population than in the BH population. The results of pedigree and genetic structure research indicate the need for a comprehensive approach to monitoring to improve future breeding and genetic diversity in the BH and SRB Lipizzan populations.

This study elucidates the expression dynamics and biological functions of oar-miR-214_3p in ovarian and uterine tissues of Liangshan Black Sheep across distinct stages of the oestrous cycle, providing novel insights into its regulatory mechanisms governing ovine reproductive physiology. The study employed RT-qPCR, northern blotting, FISH, dual-luciferase reporter assays, and ELISA to analyse the expression and biological functions of oar-miR-214_3p in the ovary and uterus of Liangshan Black Sheep across different stages of the oestrous cycle (proestrus, oestrus, metoestrus, and dioestrus phases). RT-qPCR and northern blotting revealed that oar-miR-214_3p is expressed in the ovarian and uterine tissues, with significant variations across different stages. Compared with the dioestrus and metoestrus phases, oar-miR-214_3p expression was significantly increased during the proestrus and oestrus phases (P < 0.05), with the highest levels observed during oestrus (P < 0.05). FISH analysis indicated that oar-miR-214_3p is primarily localised in the cytoplasm. We constructed wild-type and mutant vectors for mammalian target of rapamycin(mTOR) and Semaphorin 4D (Sema4D). After intervention for 48 h in granulosa cell cultures, RT-qPCR analysis of mTOR and Sema4D expression revealed that the mimic and inhibitor groups suppressed and promoted the expression of these target genes, respectively. The control and NC groups showed stable expression levels with no significant differences. Dual-luciferase reporter assay confirmed that mTOR and Sema4D are oar-miR-214_3p target genes. ELISA revealed that the mimic and inhibitor treatment groups promoted and suppressed oestrogen secretion, respectively. These findings confirm that oar-miR-214_3p, primarily localised in the cytoplasm, regulates ovarian follicle development, ovulation, oestrogen secretion, and the oestrous cycle via its target genes, mTOR and Sema4D. The study provides valuable insights into improving sheep reproductive performance through molecular breeding techniques. These findings have significant clinical and practical applications for enhancing reproductive efficiency.

This study evaluated the effects of feeding European seabass (Dicentrarchus labrax) that developed from the fingerling to juvenile stage a diet supplemented with a blend of Padina sp. and GroBiotic^®-A over a 12-week period. A total of 480 fish (initial weight: 2.08 ± 0.06 g) were randomly allocated to four groups, each comprising three replicates. The fish were fed to satiation with diets containing varying proportions of Padina sp. and GroBiotic^®-A: 0% Padina sp. and 0% GroBiotic^®-A (0P0G), 1% Padina sp. and 1% GroBiotic^®-A (1P1G), 2% Padina sp. and 1% GroBiotic^®-A (2P1G), and 4% Padina sp. and 1% GroBiotic^®-A (4P1G). The 4P1G group demonstrated significant improvements in growth performance parameters, including final weight, weight gain, and specific growth rate (P < 0.05). However, no significant differences were found in other parameters including feed conversion ratio, visceral somatic index, and survival rate (P > 0.05). Histological analysis of liver and intestinal tissues showed no pathological alterations (P > 0.05); more likely, only adaptive and non-pathological morphological changes such as moderate lipid vacuolisation and preserved mucosal structure were noted. Furthermore, dietary supplementation of the Padina sp. and GroBiotic^®-A blend significantly upregulated the expression of growth-related genes, specifically growth hormone and insulin-like growth factor 1 (P < 0.05). These results suggest that the combination of Padina sp. and GroBiotic^®-A has a synergistic potential to enhance the growth performance without inducing digestive disturbances. Nevertheless, further studies are recommended to assess its effects on fish grown to market size before commercial application.

This study investigated the effects of dry cow feeding on colostrum quality. A survey was conducted, and samples of colostrum and feed ingredients were collected from 25 dairy farms in Northern Italy. Colostrum was analysed for quality in terms of Brix value and gamma globulins, while diets and feed ingredients were analysed for their chemical constituents. The mean colostrum quality (n = 163) was 24.3 ± 4.41% Brix and 54.2 ± 20.8 g/l gamma globulin. The refractometer method effectively assessed colostrum quality, correlating accurately with laboratory gamma globulin measurements (R = 0.729). Factor analysis indicated that key nutrients, such as protein and fat daily intakes in dry cow diets, influenced colostrum immunoglobulin G. Furthermore, supplementation of selenium, vitamin A, vitamin B12, and vitamin E also improved colostrum quality. Colostrum feeding occurred, on average, 5.44 ± 2.63 h post-calving, with a quantity of 2.74 ± 0.71 l per meal, which is below the recommended 10% of the calf body weight. However, the maximum feeding time was 12 h, and the minimum colostrum quantity was 1.25 l, highlighting considerable room for improvement.

This study aimed to evaluate the effects of dietary administration of peppermint (Mentha piperita) alone and in combination with southernwood (Artemisia abrotanum) on performance, body and carcass composition, physicochemical characteristics, and amino acid composition of breast meat in broilers under Eimeria challenge. A total of 160 14-day-old male broilers (Ross 308) were randomly assigned to four dietary groups: the control (C) group was fed a basal diet; the CC group received a grower diet containing the coccidiostat robenidine; the M group was fed the diets with the addition of 20 g peppermint/kg of diet; the MS group was fed the diets with the addition of 10 g peppermint + 10 g of southernwood/kg of diet. The addition of peppermint to the diet increased final live weight compared to the CC group (P < 0.05). The highest value of dressing percentage was found in the M group as compared to the CC group (P < 0.01) and C group (P < 0.05). Compared with the C group, a higher intramuscular fat content was found in the breast meat of broilers in both the M and MS groups (P < 0.01; P < 0.05, resp.). Concerning the colour of meat, lower values for yellowness b* and chroma C* characteristics were observed in the C group compared to the MS group (P < 0.05) and mainly to the M group (P < 0.01). In spite of the lower crude protein content (P < 0.05) in the meat of the MS group, the highest content of all essential amino acids (P < 0.05) as well as the total content of all assessed amino acids (P < 0.01) were found in the meat of the MS group, resulting in a considerable increase in the nutritional value of the breast meat proteins of these broilers.

The aim of this work was to evaluate the effect of different particle sizes of hens diets on blood biochemical parameters, ileal digesta viscosity and nitrogen retention. In the experimental part, the effects of different physical structure were investigated on two groups of laying hens of the Bovans Brown hybrid from 76 to 80 weeks of age. A finely ground mash diet [geometric mean diameter (GMD), 632 µm] and a coarsely ground mash diet (GMD, 1 258 µm) with the equal nutritional content were used. In the experiment, the particle sizes of the feed mixtures were analysed and compared with the particle size of unaccepted feed residues using a feed separator. Furthermore, feed consumption, live weight of laying hens, blood biochemical parameters, digestive viscosity and nitrogen retention coefficient were assessed. The study revealed that the particle sizes of hens’ diets significantly influenced the nitrogen retention coefficient, with higher values observed in the finely ground diet as compared to the coarsely ground diet (30.3 vs 24.0%; P < 0.05). However, no significant differences were observed in feed intake, live weight, blood biochemical parameters, or digesta viscosity between the dietary groups (P > 0.05). This finding highlights the potential of diet structure optimisation to improve nutrient utilisation efficiency, which is particularly relevant for reducing nitrogen excretion and its environmental impact. These novel insights provide a foundation for further research on the effects of feed structure on productivity and organ health.

Lactoferrin (LF) is an important bioactive component of colostrum, is critical for the development of immunity in the newborns, and it is an important component of the mammary gland defence system. LF is also an important biomolecule in terms of promoting and restoring the human health. The aim of our study was to monitor the dynamics of changes in lactoferrin concentration in goat milk at varying stages of lactation and its correlation with selected components and physicochemical parameters. Colostrum (n = 24) and milk (n = 120) samples were obtained from 12 goats by hand milking. Lactoferrin was determined using reversed-phase high-performance liquid chromatography with an ion-pairing reagent equipped with a Photodiode Array Detector. The LF concentration in colostrum ranged from 206 mg/l to 1 228 mg/l, and showed a statistically significant decrease in concentration (P < 0.05) over the colostral period. Significant correlation coefficients (P < 0.001) were found between crude protein and LF (r = 0.896), lactose and LF (r = –0.754), as well as between non-fat solids and LF (r = 0.853). The LF content in milk ranged within a relatively wide range of 94 mg/l to 1 115 mg/l although the values were highly variable (vx = 57.0%). Significant correlations were found between fat content and LF in milk (r = 0.429, P < 0.001), crude protein and LF (r = 0.376), non-fat solids and LF (r = 0.361), somatic cell count (SCC) and LF (r = 0.330), as well as log SCC and LF (r = 0.348, P < 0.01).

Lab-grown cells (also known as cell-based or cultured meat) are novel food innovations that face uncertain consumer acceptance. This review examines recent research (2020–2024) on consumer perceptions of lab-grown cells, focusing on three main areas: (1) public awareness and familiarity, (2) psychological and cultural aspects shaping perceptions, and (3) the impact of information on acceptance. Results show that the awareness of lab-grown cells varies widely across regions; in countries with high meat consumption, such as the USA and Australia, or where culinary delights are highly valued, such as France, respondents rated lab-grown cells lower than in those countries where meat consumption is now rising, for example, China or Mexico. The knowledge of lab-grown cells positively impacted attitude, while psychological barriers (e.g. food neophobia) and disgust emerged as key deterrents. The information provided to respondents significantly influenced their willingness to accept, buy, try, eat, and pay premium prices. Personal (mainly health-related) benefits significantly increased the acceptance of lab-grown cells, while societal benefits (e.g. benefits to the environment or animal welfare) had less prominent effects than expected. At the same time, information regarding production technology (high-tech, laboratory, artificial) resulted in lower ratings from respondents. The conclusion is that overlooking the positive attributes of meat and focusing on the unproven advantages of lab-grown cells may lead to misleading results. On the other hand, effective communication – especially messages highlighting personal benefits – can substantially improve the consumer openness.

Selenium (Se) supplementation is a common practice in dairy nutrition. However, the use of biofortified feedstuffs remains a not fully realized strategy to enhance the Se content of animal derived products. This study explored an on-farm biofortification approach by incorporating Se-enriched maize silage into the total mixed ration (TMR) of dairy cows. Sixty Holstein cows were divided into a control group (CON), receiving a conventional diet with selenite supplementation (0.6 mg/kg Se in TMR), and an experimental group (EXP), in which conventional silage was replaced with high-Se silage (0.9 mg/kg Se in TMR). The trial lasted 22 weeks, including one week of adaptation and four weeks after supplementation, when Se concentrations in milk, Se transfer efficiency, and key milk components critical for the production of Se-enriched dairy products were assessed. The higher Se concentration in the TMR had no adverse effects on milk composition or antioxidant status. Milk Se concentration in the EXP group increased rapidly, reaching 68 µg/l within two weeks, significantly higher (P < 0.005) than in the CON group (27 µg/l). Se transfer efficiency to milk was also higher in the EXP group (13.9%) compared to the CON group (8.8%). The diverse Se species in biofortified silage, confirmed through the speciation analysis, may have contributed to these outcomes. However, the gradual decline in milk Se after the initial peak warrants further investigation into physiological factors or changes in silage Se speciation during storage.

The objective of this study was to investigate the effect of papaya peel addition on the quality and in vitro ruminal digestion of napier grass silage. Fresh napier grass was mixed with papaya peel at 0% (control), 15%, 30%, and 45% of fresh weight and ensiled under anaerobic conditions. After fermentation, silages were analysed for pH, organic acids, ammonia-N, and nutrient composition. In vitro ruminal fermentation was conducted using the rumen fluid collected from buffaloes to assess gas production, volatile fatty acid profiles, and hydrogen release. The results showed that papaya peel addition significantly affected silage fermentation and nutrient composition. The inclusion at 15% lowered pH and increased lactic acid concentration compared with the control (P < 0.05). Higher inclusion levels (30% and 45%) reduced neutral detergent fibre and acid detergent fibre content, increased total digestible nutrients and relative feed value (P < 0.05). In vitro ruminal fermentation showed that, compared with the control, hydrogen production was significantly reduced in the 15% treatment, and the acetate-to-propionate ratio was highest in the 45% treatment as a result of decreased propionate acid concentration (P < 0.05). Our results suggested that co-ensiling napier grass with papaya peel improved fermentation quality and feed value, with 15% addition yielding the most desirable balance of fermentation characteristics and ruminal fermentation outcomes. This approach provides a practical strategy for enhancing the forage preservation and utilising fruit-processing by-products in tropical livestock systems.

RE-NUM-OR is a new, flexible, and user-friendly renumbering and reordering data arrangement software for unprocessed pedigree files used in genetic evaluation systems for personal computers. RE-NUM-OR was written using Python (Ver. 2.7.13) 64 byte programming language and compiled with PyInstaller-3.2.1 software which is a set of utilities for freezing Python scripts into executable. RE-NUM-OR not only runs on 32 or 64 byte MS Windows but also runs on 64 byte GNU/Linux. The program has new, practical, and simple interface and the user does not need to create a parameter file for running the program and .txt, .xlsx or .et extension files can be used as input files directly. Output files (.txt, .xlsx, .et, .dat) can be obtained. For animal, sire and dam IDs, it can read all standard characters (ASCII codes from 32 to 126) in input files. The program supports both dot (.) and comma (,) for numerical data. Pedigree lines for parents do not need to be arranged prior to their progeny. If pedigree lines for parents follow their progeny, the program can detect this and it can reorder the animals as pedigree lines placing parents prior to their progeny. Another feature of this software is that it has a pop-up window with error notification. Also, it supports repeated observations. RE-NUM-OR executable, its user manual, and sample input files are available from www.kemalyazgan.com.tr.

This study investigates advancements and trends in Fleckvieh breeding research using the Web of Science Core Collection database to collate articles published since 1994. Through CiteSpace 6.4.R1, we conducted systematic analyses to visualise research trajectories, identify seminal domains, and delineate emerging patterns. Our methodology encompassed publication profiling, scientific collaboration network mapping, co-citation analysis, and keyword co-occurrence/clustering techniques. The findings reveal that the current research frontiers focus on: Genomic applications in genetic selection, Reproductive efficiency optimisation, Environmental adaptability mechanisms, Thermotolerance management strategies, Cross-disciplinary methodological integration. Germany, Czech Republic, Austria, and Switzerland emerged as leading contributors, with institutions including the Technical University of Munich and University of Natural Resources and Life Sciences, Vienna, demonstrating a particular scholarly influence. Knowledge mapping effectively delineated thematic concentrations and temporal evolution patterns, providing actionable insights for future research prioritisation. The field is progressing towards holistic, interdisciplinary frameworks underpinned by robust international consortia. This work introduces novel analytical perspectives to refine breeding protocols and enhance practical implementation.

Some herbs can be used as phytogenic additives as they naturally contain specific bioactive phytochemicals that are effective in controlling coccidiosis and some other poultry diseases due to their immunostimulatory effects and they can be also nutritious. The aim of the study was to evaluate the effect of Artemisia absinthium (WW) meal inclusion to a diet on the production performance, oocyst output and minerals content of meat in broilers infested by Eimeria oocysts. Ross 308 broilers were randomly assigned to four dietary groups. Broilers in the control group were fed basal diets without coccidiostat or WW. Broilers of the R group were fed starter and grower diets supplemented with coccidiostat robenidine. Broilers in the W3 and W6 groups were fed diets containing 3 and 6% of WW in feed, resp. At the age of 16 days, all broilers were infested by oocysts of Eimeria spp. to induce a mild coccidiosis infection. The WW meal had no effect on the production performance and carcass traits of broilers (P > 0.05). However, the used levels of administered WW meal were not effective in reducing the number of oocysts faecal output in infested broilers. Administration of coccidiostat robenidine prevented the development of Eimeria oocysts in the intestine as long as 4 days after the cessation of feeding the medicated diet to broilers (P < 0.05). As for minerals of breast meat, broilers of the W3 group displayed a higher Ca level compared to those in the R group (P < 0.05). On the contrary, the R group showed the highest Cu level compared to all other dietary groups assessed (P < 0.01). It can be concluded that for the coccidiosis control in poultry production, it is advisable to monitor the content of specific bioactive substances with proven anticoccidial activity when using WW products.

Monitoring the genetic diversity in livestock is a critical component for sustainable management of small and endangered breeds. In this paper we follow up the two most often used metrics of genetic diversity, the average inbreeding coefficients (F) and the effective population (N_e) size in 16 sheep breeds from four countries. The analysis was based on single nucleotide polymorphism (SNP) markers, with about 35 to 49 thousand SNPs and 19 to 98 individuals after quality control. The runs of homozygosity (ROH) method with the cgaTOH software was used to estimate F_ROH, for three different time points in the past (3, 6 and 12 generations before present). Three methods were implemented to estimate N_e, using the NeEstimator v2, GONE and CurrentNe software for different time spans in the past. The average F_ROH ranged between 0.001 and 0.035 for 3 generations, 0.003 and 0.059 for 6 generations, 0.005 and 0.074 for 12 generations in the past. The wide range of inbreeding coefficients might be a consequence of different population sizes and breed management strategies in the respective countries. The results for N_e ranged from 7 to 352 for NeEstimator, 25 to 303 for GONE, and 15 to 542 for CurrentNe. Overall, the software showed comparable results for 10 out of 16 breeds, and significantly different results for at least one software for at least one of the methods for 6 out of 16 breeds. These differences show a degree of uncertainty in N_e estimations for certain breeds. Overall, we suggest the continued monitoring of sheep breeds, ideally by routine genotyping in all populations. This monitoring is especially important for small breeds with a greater decline in genetic diversity.

Raw cow’s milk is one of the most strictly controlled commodities in the food industry. Besides basic hygienic and nutritional parameters, the presence of inhibitory and contaminant substances plays a critical role in technological quality and safety. While the vast majority of inhibitory residues are represented by antibiotics originating from mastitis prevention and therapy, milk also contains a broad spectrum of naturally occurring native antimicrobial compounds. These include immunoglobulins, lactoferrin, lysozyme, lactoperoxidase, phosphatases, lipases, and proteases acting as integral elements of the innate immune system within the mammary gland. Although these substances are not legislatively regulated, they significantly influence milk processing, starter culture growth, cheese yield, and shelf-life of final dairy products. Their concentration is strongly associated with animal health status, especially mastitis, and may thus also serve as an indirect quality indicator. This review provides a comprehensive overview of native inhibitory compounds in bovine milk, their concentration ranges, biological significance, impact on dairy technology, and the analytical methods currently used for their detection.

This study evaluated test-day records of clinical mastitis (CM), somatic cell count, and nine udder conformation traits. Somatic cell count was log-transformed into somatic cell score (SCS) in 10 periods, each 30 days long and overall, for the first lactation. CM is a complex disease closely connected with somatic cell count. The optimum udder conformation traits significantly affect dairy cattle health. The CM binary trait was monitored in seven periods throughout lactation, each 50 days long, and for the whole lactation. A logistic regression model was used to estimate the risk of CM. The model included a fixed effect of herd-year-season, age at first calving, and a fixed effect of the linear type traits of the random effect of the animal. The phenotypic correlations for udder conformation traits, CM, and SCS ranged from –0.13 to 0.69 and standard errors were 0.01–0.99. The highest CM incidence and SCS were observed for the medial ligament scores 1–2: convex base of the udder. According to the logistic regression assessment, the medial ligament scores 1–2: convex base of the udder and the CM incidence to 50 days in milk reported a 3.79 times higher probability of the CM incidence at the reference level (extremely deep medial ligament) at the same stage of the lactation. CM incidence and SCS significantly decreased with decreasing udder depth. Udder depth below the hock was associated with the highest risk of CM. For udder depth and the whole lactation, the CM ODDS ratio was 1.00–2.56, CM least squares means were 0.18–0.44, and SCS least squares means were 3.20–4.10. Our study confirmed that the start of lactation is critical for the onset of CM, and somatic cell count is manifested throughout lactation. The effect of the udder conformation is then observable in somatic cell count and CM during the whole lactation.

Since 2020, the National Agricultural Research Institute (INIA) has conserved a Creole sheep flock at INIA Las Brujas. This study genetically characterised this population and compared it with six others: San Miguel National Park Creoles, commercial farms Creoles, Brazilian Creoles, Corriedale, Merilin and Soay sheep. The analysis included 628 individuals and 31 392 autosomal SNPs. Soay and San Miguel National Park Creoles exhibited the lowest genetic diversity (Ho = 0.266 and 0.279) and highest inbreeding (F_HOM: 0.283 and 0.249; F_ROH: 0.199 and 0.202). Merilin and Corriedale showed the highest genetic diversity (Ho = 0.351 and 0.364), while Brazilian Creoles had the highest Ho (0.327) among Creoles. Short runs of homozygosity (ROH) segments (≤4 Mb) predominated, with San Miguel National Park and INIA Las Brujas Creoles exhibiting the highest numbers of ROH (22 773 and 16 762, respectively). Fixation index (Fst) and Reynolds distances highlighted INIA Las Brujas Creoles and Soay as the most distinct (0.318 and 0.321, respectively). INIA Las Brujas Creoles also showed notable differentiation from San Miguel National Park Creoles (Fst = 0.269; Reynolds = 0.272). Principal component analysis (PCA) revealed clear clustering, with Corriedale and Merilin closely related (Fst = 0.060; Reynolds = 0.068). Admixture analysis indicated distinct ancestries for Soay, Corriedale and San Miguel National Park Creoles, while commercial and Brazilian Creoles showed significant admixture. INIA Las Brujas Creoles exhibited a distinct ancestry with traces of Corriedale. Phylogenetic analysis confirmed the divergence between Creole and Corriedale/Merilin populations. Results suggest the historical genetic exchange among INIA Las Brujas Creoles, Corriedale, and commercial farms Creoles, while high differentiation of San Miguel National Park Creoles reflects their closed status since 1929.

This study aimed to evaluate the effects of dietary levels of soluble and insoluble fibre on the performance and digestive development of slow-growing broilers during the starter period. A total of 400 one-day-old Isa Label^TM male chicks were used that were distributed in a completely randomized factorial design 2 × 2 + 1 (inclusion of 2% or 4% of corncobs as a source of insoluble fibre IF and citrus pulp as a source of soluble fibre (SF); and a control treatment) with eight replicates of 10 birds each. The addition of IF to diets improved the weight gain and feed conversion of broilers at 7 and 21 days of age. Both fibre sources added to diets resulted in higher relative weights of proventriculus + gizzard, liver, small and large intestine. The diets containing an SF source presented positive effects on the duodenal mucosa. In addition, positive effects on the caecal villus to crypt ratio were observed in broilers fed diets containing different types of fibre. It is concluded that dietary inclusion of IF up to 4% maintains the performance, nutrient metabolism and improves the development of the proventriculus + gizzard in slow-growing broilers. However, for improving the morphological and histomorphological parameters of slow-growing broilers in the starter phase the addition of SF sources is recommended.