Czech Journal of Animal Science, 2017 (vol. 62), issue 7

Two single nucleotide polymorphisms in the caprine GnIH gene are associated with litter sizeOriginal Paper

Xiaopeng An, Lijuan Bao, Jinxing Hou, Yueyu Bai, Xinyan Zhao, Yuxuan Song, Binyun Cao

Czech J. Anim. Sci., 2017, 62(7):269-275 | DOI: 10.17221/58/2016-CJAS  

Gonadotropin-inhibitory hormone (GnIH) can decrease luteinizing hormone and/or follicle-stimulating hormone levels in rat, mouse, sheep, and cattle by the direct suppression of gonadotropin-releasing hormone (GnRH). The present study investigated polymorphisms in the GnIH genes of two dairy goat breeds and their association with litter size. Single nucleotide polymorphisms (SNPs) g.1837C>G and g.3195G>A (GenBank Accession Nos. KR778885 and KR819142) were detected in the GnIH genes of Xinong Saanen and Guanzhong dairy goat breeds using DNA sequencing and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)....

Genetic evaluation for functional longevity in Polish Simmental cattleOriginal Paper

Małgorzata Morek-Kopeć, Andrzej Zarnecki

Czech J. Anim. Sci., 2017, 62(7):276-286 | DOI: 10.17221/87/2016-CJAS  

The Weibull proportional hazards model was applied for genetic evaluation of functional longevity in Polish Simmentals. Data consisted of production and disposal records for 12 527 Simmental cows, daughters of 294 sires, calving for the first time from 1995 to 2014 in 286 herds. Length of productive life of cows was calculated as number of days from the first calving to culling or censoring. Average length of productive life of 4462 cows with complete (uncensored) survival records was 1198 days (39.3 months); mean censoring time for the remaining 8065 cows was 1093 days (35.8 months). Functional longevity was defined as length of productive life corrected...

Effect of dietary supplement of herbal extract from hop (Humulus lupulus) on pig performance and meat qualityOriginal Paper

Ewa Hanczakowska, Małgorzata ¦wi±tkiewicz, Eugeniusz Ryszard Grela

Czech J. Anim. Sci., 2017, 62(7):287-295 | DOI: 10.17221/49/2016-CJAS  

The study deals with the effect of diets supplemented with a hop extract on growing pigs. Two levels of the extract (500 or 1000 mg/kg of diet) were used. Feeds either unsupplemented or supplemented with butylated hydroxytoluene were used as negative and positive controls. Totally 96 pigs weighing ca. 60 kg were allocated to 4 groups. They were fed individually with restricted feed amounts. Water was available ad libitum. Body weight gain, apparent digestibility of nutrients, carcass and meat quality were estimated and biochemical indices in the blood were analyzed. The herbal extract decreased digestibility of fat and its higher dose also...

Identification and characterization of bovine mammary peptide transporters in response to tripeptide and lactogenic hormone treatmentOriginal Paper

Yan Cui, Xingfu Zhang, Chunli Guo, Ruiping Du, Gaowa Ailun, Changjin Ao, Min Gao

Czech J. Anim. Sci., 2017, 62(7):296-305 | DOI: 10.17221/93/2015-CJAS  

Oligopeptide transportation is mediated by the peptide transporter (PepT), which consists of two isoforms, PepT1 and PepT2. Because PepT play essential roles in amino acid metabolism and cell growth, the aim of the present study was to identify these transporters in bovine mammary glands and to analyze the potential functions of these transporters in mammary epithelial cells. Abundance of PepT1 and PepT2 mRNA was successfully measured in both mammary glands and cultured mammary epithelial cells. In addition, the two proteins were examined using immunohistochemistry, immunocytochemistry, and Western blots. The response of mammary epithelial...

Effects of biological and chemical additives on fermentation progress in maize silageOriginal Paper

Yvona Tyrolová, Luděk Bartoň, Radko Loučka

Czech J. Anim. Sci., 2017, 62(7):306-312 | DOI: 10.17221/67/2016-CJAS  

The objective of this study was to evaluate the effects of bacterial and chemical additives on the number of lactic acid bacteria (LAB) and on fermentation indicators in whole maize silage at 1, 3, 5, 10, and 90 days of fermentation. Maize forage was harvested at approximately 34% dry matter (DM) and treated with (1) no additive (control; C); (2) bacterial inoculant (2 g/t of forage; B) containing the homofermentative LAB Lactobacillus plantarum, Lactobacillus paracasei, and Pediococcus pentosaceus (1.5 × 1011 cfu/g of inoculant); and (3) chemical additive (4 l/t of forage; CH) containing formic acid, propionic...