Czech Journal of Animal Science, 2016 (vol. 61), issue 11

Genome-wide analysis of CpG islands in some livestock genomes and their relationship with genomic featuresOriginal Paper

A. Barazandeh, M.R. Mohammadabadi, M. Ghaderi-Zefrehei, H. Nezamabadi-pour

Czech J. Anim. Sci., 2016, 61(11):487-495 | DOI: 10.17221/78/2015-CJAS  

CpG islands (CGIs) are an important group of CpG dinucleotides in the guanine- and cytosine-rich regions as they harbour functionally relevant epigenetic loci for whole genome studies. As a matter of fact, since there has not been a formal comparative analysis of CGIs in domestic even-toed ungulate genomes, this study was performed to serve this comparison. The Hidden Markov Model was used to detect CGIs in the genomes. The results indicated that the CGIs number and CGI densities had scant variations across genomes. The goat genome had the highest number of CGIs (99 070), whereas the alpaca genome had the highest CGI density (43.39 CGIs/Mb). Significant...

Effect of selenium-enriched defatted rape seeds on tissue cadmium and essential elements utilization in ratsOriginal Paper

A. Myška, J. Száková, A. Fučíková, P. Mlejnek, V. Zídek, J. Tremlová, O. Mestek, R. Koplík, J. Zídková, M. Melčová, P. Tlustoš

Czech J. Anim. Sci., 2016, 61(11):496-505 | DOI: 10.17221/88/2015-CJAS  

The effect of selenium-enriched defatted rapeseeds (DRS) added to the diet on Se uptake by rat organisms (whole blood, liver, kidney) was investigated in model conditions. Additionally, the response of other essential trace and major mineral elements (Cu, Ca, Fe, K, Mg, Mn, P, S, and Zn) in rat organisms on selenium and/or DRS addition was assessed. The experimental diets for the individual experimental groups were prepared as follows: group DRS 0 (control diet), group DRS 30 (30% of soybean meal in the diet replaced with DRS), group DRS60 (60% of soybean meal in the diet replaced with DRS), and group DRS100 (100% of soybean meal in the diet replaced...

In vitro fermentation pattern in the large intestine of hybrids between wild boars and domestic pigs - a preliminary studyOriginal Paper

D. Miśta, B. Króliczewska, E. Pecka-Kiełb, J. Bujok, W. Zawadzki, J. Górecka, J. Piekarska

Czech J. Anim. Sci., 2016, 61(11):506-514 | DOI: 10.17221/7/2016-CJAS  

Breeding of hybrids between wild boars and domestic pigs is in the consumer interest because of the need to ensure food security and diversification via widening the genetic basis of animals reared for meat. To expand the knowledge about their nutritional requirements, this study aimed to investigate hindgut fermentation in these animals. Caecal and colon cultures were incubated for 12 h in vitro with or without wheat bran as a supplementary substrate. Short-chain fatty acids, ammonia, methane, and total gas production were determined. The total concentrations of short-chain fatty acids in unincubated caecal and colon samples were 93.1 and...

Equilibration and freezing interactions affecting bull sperm characteristics after thawingOriginal Paper

M. Doležalová, L. Stádník, Z. Biniová, J. Ducháček, R. Stupka

Czech J. Anim. Sci., 2016, 61(11):515-525 | DOI: 10.17221/23/2016-CJAS  

The objective was to determine effects of equilibration length and freezing curve type as well as their interactions on motility and live spermatozoa proportion in bull sperm after thawing. The ejaculates of 6 sires were repeatedly collected. Fresh semen was diluted with one extender and divided into 3 groups equilibrated for 30, 120, and 240 min. Subsequently, half straws of each group were frozen using standard 3-phase or 2-phase freezing curve differing in the rate of temperature decrease. The spermatozoa motility (M) was evaluated immediately after thawing and at 30, 60, 90, and 120 min of thermodynamic test (TDT). Live spermatozoa proportion was...

Using gene networks to identify genes and pathways involved in milk production traits in Polish Holstein dairy cattleOriginal Paper

T. Suchocki, K. Wojdak-Maksymiec, J. Szyda

Czech J. Anim. Sci., 2016, 61(11):526-538 | DOI: 10.17221/43/2015-CJAS  

When analyzing phenotypes undergoing a complex mode of inheritance, it is of great interest to switch the scope from single genes to gene pathways, which form better defined functional units. We used gene networks to search for physiological processes and underlying genes responsible for complex traits recorded in dairy cattle. Major problems addressed included loss of information from multiple single nucleotide polymorphisms (SNPs) located within or close to the same gene, ignoring information on linkage disequilibrium and validation of the obtained gene network. 2601 bulls genotyped by the Illumina BovineSNP50 BeadChip were used. SNP effects were...