Czech Journal of Animal Science, 2015 (vol. 60), issue 10

Effect of green alga Planktochlorella nurekis on selected bacteria revealed antibacterial activity in vitro Original Paper

L. Čermák, Š. Pražáková, M. Marounek, M. Skřivan, E. Skřivanová

Czech J. Anim. Sci., 2015, 60(10):427-435 | DOI: 10.17221/8522-CJAS  

The green alga Planktochlorella nurekis (Chlorellaceae, Chlorophyta) is considered a producer of antibacterial mixture of long-chain fatty acids, which has possibly similar composition and mode of action as chlorellin produced by another green alga, Chlorella vulgaris. Although the antibacterial properties of C. vulgaris have been reported, the interactions of P. nurekis with bacteria have not been determined yet. The aim of this study was to elucidate the effect of P. nurekis water suspension on growth of selected gastrointestinal bacteria in vitro so that it could be used as a suitable feed supplement...

Egg surface decontamination with bronopol increases larval survival of Nile tilapia, Oreochromis niloticusOriginal Paper

S. Jantrakajorn, J. Wongtavatchai

Czech J. Anim. Sci., 2015, 60(10):436-442 | DOI: 10.17221/8523-CJAS  

Intensive tilapia egg incubation techniques create favourable conditions for microbial proliferation which often leads to massive mortalities of fish larvae. The effects of Nile tilapia (Oreochromis niloticus) eggs exposure to bronopol on decontamination of their bacterial surface and survival of larvae were observed. Immersion treatments of fertilized eggs were applied at 10, 25, 50, 100, and 250 mg/l of bronopol for 10, 20, and 30 min. This treatment substantially reduced the number of bacteria on Nile tilapia eggs. The greatest reduction in bacterial numbers (1.58 × 10³ colony forming units/g of egg) was observed at the maximum...

Characterization of greying, melanoma, and vitiligo quantitative inheritance in Old Kladruber horsesOriginal Paper

B. Hofmanová, L. Vostrý, I. Majzlík, H. Vostrá-Vydrová

Czech J. Anim. Sci., 2015, 60(10):443-451 | DOI: 10.17221/8524-CJAS  

The paper deals with the greying process, melanoma, and vitiligo depigmentation occurrence in Old Kladruber horses (OKH). The study includes 376 animals of both sexes at the age of 1-25 years. The evaluation was performed repeatedly during 4 consecutive years. The greying status was measured spectrophotometrically on four body parts using L*a*b* colour system. Melanoma and vitiligo were detected visually and by palpation in all the animals and classified using 5 (3) grade scale. The GLM procedure of SAS package was used to examine the influence of the effects of line, age, sex, stud, and year of evaluation. The GLM analysis...

Tissue-specific mRNA expression profiles of GDF9, BMP15, and BMPR1B genes in prolific and non-prolific goat breedsOriginal Paper

Z.Y. Pan, R. Di, Q.Q. Tang, H.H. Jin, M.X. Chu, D.W. Huang, J.N. He, Q.Y. Liu, W.P. Hu, X.Y. Wang, Y.X. Yao, L. Liu, C.L. Song

Czech J. Anim. Sci., 2015, 60(10):452-458 | DOI: 10.17221/8525-CJAS  

The tissue-specific mRNA expression profiles of GDF9, BMP15, and BMPR1B genes in goats were investigated. The mRNA expression of the genes was detected in prolific Jining Grey goats and non-prolific Liaoning Cashmere goats by reverse transcription polymerase chain reaction (RT-PCR) and quantitative real-time PCR. The results showed that GDF9 and BMPR1B were widely expressed in 20 tissues, while BMP15 gene was expressed exclusively in ovary and pituitary. The expression levels of GDF9 and BMPR1B genes were the highest in ovary. The expression level of BMP15 gene in the...

Influence of long-term exposure to lead on its accumulation and elimination from tissues and on selected reproductive parameters in the Prussian carp (Carassius gibelio B.) in pond environmentOriginal Paper

E. Łuszczek-Trojnar, E. Drąg-Kozak, M. Socha, P. Szczerbik, W. Popek

Czech J. Anim. Sci., 2015, 60(10):459-472 | DOI: 10.17221/8526-CJAS  

The bioaccumulation of lead in selected tissues of Prussian carp kept in pond condition during 12 and 24 months of exposure to different doses (8, 13, 24, and 49 mg/kg) of this metal in feed and its elimination from tissues during the following 12-month depuration period was studied. Additionally gonadosomatic index and luteinizing hormone (LH) secretion, as the effect of exposure to Pb, were examined. The concentration of lead in all the studied tissues, except for the gonads, correlated positively with the metal concentration in the diet, and the maximum level was usually achieved after 3 months of the exposure. The highest levels of lead, i.e. 2.1...