Czech Journal of Animal Science, 2014 (vol. 59), issue 6
Oral delivery of insulin-loaded nanoparticles in diabetic rabbits and in sheepOriginal Paper
S. Olya, M. Khorvash, H.R. Rahmani, S. Esmaeilkhanian, B. Olya, H. Sadri
Czech J. Anim. Sci., 2014, 59(6):251-256 | DOI: 10.17221/7495-CJAS 
The objective of this study was to produce Eudragit® RS 100 microparticles (ERS) containing insulin (ILNP), and to evaluate the potential of these nanoparticles as a drug carrier for oral administration in alloxan-induced diabetic rabbits and in sheep. After oral administration of ILNP to diabetic rabbits, a significant hypoglycemic effect was observed and this effect lasted for 2 days. Concentrations of blood glucose were significantly decreased in ILNP-treated sheep compared to those in control and ERS groups on day 5 of the experiment. Orally ILNP-treated sheep exhibited higher concentrations of progesterone compared to control and...
Genetic distances between horse breeds in Poland estimated according to blood protein polymorphismOriginal Paper
A. Stachurska, A. Nogaj, A. Brodacki, J. Nogaj, J. Batkowska
Czech J. Anim. Sci., 2014, 59(6):257-267 | DOI: 10.17221/7496-CJAS
The objective of the study was to estimate the heterozygosity and phylogenetic relationship between horse breeds in Poland, according to erythrocyte antigens and protein polymorphisms. The study included 15 434 horses: Polish Coldblood, Małopolski, Wielkopolski, Hucul, Polish Konik, Biłgorajski, Felin Pony, and ponies of Shetland origin. A total of 14 loci were studied: seven blood groups and seven protein polymorphism systems. Phylogenetic trees obtained for the erythrocyte antigens and protein variants were mostly alike which suggests that both kinds of markers may be equally used in estimating the similarity of animal populations. The lower polymorphism...
Effect of Lactobacillus plantarum LS/07 in combination with flaxseed oil on the microflora, enzymatic activity, and histological changes in the development of chemically induced precancerous growth in the rat colonOriginal Paper
L. Strojný, J. Štofilová, E. Hijová, V. Szabadosová, R. Salaj, I. Bertková, A. Chmelárová, D. Čokášová, B. Pramuková, A. Brandeburová, A. Bomba, N. Bobrov, P. Suchánek
Czech J. Anim. Sci., 2014, 59(6):268-277 | DOI: 10.17221/7497-CJAS
This experimental study showed the effect of L. plantarum LS/07 on the changes in total counts of coliform bacteria, enzymatic activities, cytokine and histological changes in the colonic mucosa, chemically induced by the application of 1.2-dimethylhydrazine dihydrochloride (1.2-DMH) in rats once a week during five weeks in the positive control group, probioticum (PRO) group, and probioticum and oil (PRO-O) group. A total of 32 rats were randomly assigned to four treatment groups: negative control group (diet without any supplements), positive control group (1.2-DMH without any supplements), PRO group (L. plantarum LS/07 at the dose...
Nutritive value of maize and sorghum silages: fibre fraction degradation and rumen microbial density in buffalo cowsOriginal Paper
F. Sarubbi, A. Chiariotti, R. Baculo, G. Contò, S.A. Huws
Czech J. Anim. Sci., 2014, 59(6):278-287 | DOI: 10.17221/7498-CJAS
Sorghum could be a potential substitute to maize in Mediterranean buffaloes feed in order to improve sustainability of buffalo-based agriculture, due to its reduced water and nitrogen requirements compared with maize, which is currently fed primarily. The aim of this study is to obtain information on rumen degradability of fibre fraction of maize and sorghum silages and to investigate the relationship between degradability and rumen microbial populations. As such four cannulated buffalo milking cows were fed ad libitum two different iso-energetic and iso-proteic diets based on maize silage (MS) and sorghum silage (SS). Based on plate counts,...
Characterization of proteins in feeds according to the CNCPS and comparison to in situ parametersOriginal Paper
M. Chrenková, Z. Čerešňáková, M.R. Weisbjerg, Z. Formelová, M. Poláčiková, M. Vondráková
Czech J. Anim. Sci., 2014, 59(6):288-295 | DOI: 10.17221/7499-CJAS
Several methods were used for the evaluation of feed protein for rumen degradability and intestinal digestibility of rumen undegraded protein (RUP). The aim of this work was to explore the possibility of using the Cornell method of predicting the fraction RUP. An in situ method was adopted for estimation of degradability parameters (a, b, c) and effective degradable crude protein (EDCP), and five nitrogen fractions (A, B1, B2, B3, and C) were determined according to the Cornell Net Carbohydrate and Protein System (CNCPS). Fifty-one feedstuffs - 13 cereals grains, 5 legume seeds, 3 oilseeds, 11 oilseed by-products,...