Czech Journal of Animal Science, 2010 (vol. 55), issue 8
TaqMan allelic discrimination assay for A1 and A2 alleles of the bovine CSN2 gene
I. Manga, J. Dvořák
Czech J. Anim. Sci., 2010, 55(8):307-312 | DOI: 10.17221/89/2009-CJAS 
Alleles A1 and A2 of the Bos taurus CSN2 gene are the most frequent in a number of dairy cattle breeds. In this study, a new allelic discrimination assay using TaqMan fluorogenic probes was developed to detect single nucleotide substitution characterizing the A1/A2 alleles of the CSN2 gene. The method was validated using DNA samples of known genotypes with different concentrations and the results were compared with those for the commonly used problematic ACRS-PCR. We found the TaqMan method to be more effective, 100% reliable and hundred times more sensitive for testing the CSN2 genetic marker in...
The effect of linseed diet on carcass value traits and fatty acid composition in muscle and fat tissue of fattening pigs
R. Bečková, E. Václavková
Czech J. Anim. Sci., 2010, 55(8):313-320 | DOI: 10.17221/197/2009-CJAS
The aim of the study was to evaluate the effect of linseed in the diet of fattening pigs on carcass value traits and changes in the fatty acid profile in muscle and fat tissue. Thirty crossbred gilts were divided into two groups (control C and experimental L) and fed a commercial feed mixture for fattening pigs. Ground linseed was added to a feed mixture for the experimental group. The average daily gain, lean meat percentage and intramuscular fat content were not affected by the linseed diet but pH1 value and drip loss were significantly influenced by dietary treatment (P < 0.01). The inclusion of linseed increased linoleic and alpha-linolenic...
Effects of cholecystokinin-octapeptide and cerulein on small-intestinal motility in sheep
K.W. Romański
Czech J. Anim. Sci., 2010, 55(8):321-329 | DOI: 10.17221/1692-CJAS
Cholecystokinin (CCK) affects the intestinal motility but in ruminants the question has not been entirely explored. The aim of this study was to examine the precise effects of CCK-octapeptide (CCK-OP)and its amphibian analogue, cerulein, on duodenal motor activity in unfasted rams in the course of chronic experiments. Five rams underwent the implantation of a strain gauge force transducer to the duodenal wall, and - additionally - the bipolar platinum electrodes to the duodenal bulb, distal duodenum, near the strain gauge force transducer, and proximal jejunum. During continuous motor recordings, 0.15M NaCl or CCK peptides were administrated intravenously....
The effect of season and parity order on fertility of rabbit does and kit growth
J. Tůma, E. Tůmová, V. Valášek
Czech J. Anim. Sci., 2010, 55(8):330-336 | DOI: 10.17221/317/2009-CJAS
The effect of season and parity order on doe fertility and rabbit growth was evaluated. Two hundred and fifty Hyplus® does were divided into five groups according to kindling, each in a given season of the year (September, December, February, April and June). Another five groups were separated by parity order (from the first to the fifth kindling). After weaning, each group of rabbits was fed until 77 days of age. The season of the year significantly affected service number of pregnancy, litter size, litter weight at birth, litter weight at 21 days, litter size at weaning as well as live weight at 77 days. Parity order affected service number of...
The influence of litter age, litter temperature and ventilation rate on ammonia emissions from a broiler rearing facility
M. Knížatová, Š. Mihina, J. Brouček, I. Karandušovská, J. Mačuhová
Czech J. Anim. Sci., 2010, 55(8):337-345 | DOI: 10.17221/176/2009-CJAS
The research reported in this article attempts to address the ammonia problem by quantifying the effect of several variables on ammonia concentrations and emissions. These variables include litter temperature, litter age and ventilation rate. Data was collected in a commercial tunnel-ventilated grow-out facility with deep litter, designed for 25 000 broilers, during 6 consecutive flocks. Birds were housed from hatching to approximately 40 days of age. Litter temperature and litter age were positively correlated (P < 0.0001) with the production of ammonia gas. The amount of ammonia emissions increased with the litter age (P <...
Simplified PCR analysis of a mutation in the NHEJ1 gene causing collie eye anomaly in some dog breeds
J. Dostál, P. Horák, A. Hrdlicová, A. Stratil
Czech J. Anim. Sci., 2010, 55(8):346-350 | DOI: 10.17221/259/2009-CJAS
Collie eye anomaly (CEA) is an inherited eye disease affecting development of the choroids and sclera segregating in several, mostly herding breeds of dog. Phenotypic development of the disease varies greatly in the affected animals. Genetic control of its clinical variation is unknown so far. Affected dogs share a 7.8 kb deletion in intron 4 of the NHEJ1 gene. We report here population studies of 379 dogs (Australian Shepherd, Border Collie, Rough Collie, Smooth Collie, Shetland Sheep Dog, and Nova Scotia Duck Tolling Retriever) from breeders in the Czech Republic. A simple PCR method using a PikoTM Thermal Cycler and unclotted...