Czech J. Anim. Sci., 2025, 70(7):267-275 | DOI: 10.17221/43/2025-CJAS

Effects of BRCA1 and TLR1 SNPs on milk production performance and somatic cell score in Holstein, Simmental and crossbred Holstein cattleOriginal Paper

Maja Maurić Maljković ORCID...1, Tomislav Mašek2, Marija Špehar3, Kristina Starčević4
1 Department of Animal Breeding and Livestock Production, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
2 Department of Animal Nutrition and Dietetics, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
3 Croatian Agency for Agriculture and Food, Zagreb, Croatia
4 Department of Chemistry and Biochemistry, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia

Mastitis is a complex, common and economically problematic issue in dairy cattle production. In this study, breast cancer 1 (BRCA1) and toll-like receptor 1 (TLR1) genes were taken as candidate genes for mastitis resistance. This study investigated whether BRCA1 and TLR1 genes were associated with milk production traits (daily milk yield, fat, and protein content) and somatic cell score (SCS). A total of 105 cows (25 Holstein, 48 Simmental, and 32 crossbred Holstein) were genotyped using the PCR-RFLP method. Cows with the BRCA1 c.46126G>T GG genotype had significantly lower SCS than the other genotypes. The TLR1 g.60438363C>T SNP influenced the protein content in all cows, with genotype CC having a higher content than TC. The same SNP in the Simmental breed showed that cows with the CC genotype had significantly higher SCS than the heterozygote. Cows with the GG genotype of TLR1 g.60437324A>G had significantly lower SCS and higher fat and protein content than the heterozygote. The results of this study indicate that BRCA1 c.46126G>T and TLR1 g.60437324A>G SNPs could be useful for improving mastitis resistance in dairy cattle through marker-assisted selection.

Keywords: cow; mastitis; mastitis resistance; somatic cell count; udder health

Received: April 2, 2025; Accepted: July 8, 2025; Published: July 29, 2025  Show citation

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Maurić Maljković M, Mašek T, Špehar M, Starčević K. Effects of BRCA1 and TLR1 SNPs on milk production performance and somatic cell score in Holstein, Simmental and crossbred Holstein cattle. Czech J. Anim. Sci. 2025;70(7):267-275. doi: 10.17221/43/2025-CJAS.
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