Czech J. Anim. Sci., 2024, 69(11):450-461 | DOI: 10.17221/98/2024-CJAS

Dietary antioxidant supplementation improves the in vitro quality and antioxidant capacity of Colombian Creole stallion semenOriginal Paper

Maria Claudia Puerta1, Laura Henao-Salazar1, Isabel Catalina Vélez1, Sergio León2, Banjamín Rojano3, Giovanni Restrepo4, Alexandra Úsuga ORCID...5
1 Faculty of Veterinary Medicine and Animal Science, CES University, Medellín, Colombia
2 Faculty of Agricultural Science, University of La Salle Universidad Nacional de Colombia, Bogotá, Colombia
3 Faculty of Science, the university Universidad Nacional de Colombia, Medellín, Colombia
4 Faculty of Agricultural Science, the university Universidad Nacional de Colombia, Medellín, Colombia
5 Biogenesis Research Group, Faculty of Agricultural Science, University of Antioquia, Medellín, Colombia

Dietary antioxidant supplementation has become popular for managing male infertility because of its impact on the antioxidant capacity of semen. This study evaluates the effect of dietary antioxidant supplementation on the seminal characteristics of Colombian Creole horses. Semen from six Colombian Creole stallions was collected using an artificial vagina. The stallions were randomly allocated to a control group (CG) or a supplementation group (SG) that received a multi-antioxidant supplement. A repeated measures design, with a wash out period of two weeks between treatments, was used. Sperm motility and kinematics were assessed using a computer-assisted semen analysis system, membrane integrity (MI) and the acrosomal integrity (AI) of sperm were assessed using fluorescent microscopy, abnormal morphology (AM) was analysed using supravital staining, and the functionality of the sperm membrane was assessed using the hypoosmotic swelling (HOS) test. A fraction of each ejaculate sample was centrifuged to obtain seminal plasma (SP). The reactive oxygen species and the total antioxidant capacity (TAC) of the SP were measured via spectrofluorimetry. Linear models were fitted, and comparison of means was performed using Tukey’s test. Dietary antioxidant supplementation increased sperm beat cross frequency. In addition, a higher AI was found for the SG. Supplementation also increased the TAC of the SP. Individual analysis revealed that dietary antioxidant supplementation increased the total and progressive motility of the semen of two horses in the SG; however, supplementation reduced these two parameters in two other individuals compared to the CG. It can be concluded that dietary antioxidant supplementation with a nutraceutical that provides vitamins, minerals, amino acids, and omega-3 for horses can increase sperm quality as well as the TAC of SP; however, this may be subject to the individual effect of each horse.

Keywords: equine; nutraceutical; oxidative stress; sperm

Received: June 13, 2024; Accepted: October 20, 2024; Prepublished online: November 8, 2024; Published: November 28, 2024  Show citation

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Puerta MC, Henao-Salazar L, Vélez IC, León S, Rojano B, Restrepo G, Úsuga A. Dietary antioxidant supplementation improves the in vitro quality and antioxidant capacity of Colombian Creole stallion semen. Czech J. Anim. Sci. 2024;69(11):450-461. doi: 10.17221/98/2024-CJAS.
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