Czech J. Anim. Sci., 2024, 69(5):178-190 | DOI: 10.17221/173/2023-CJAS

In vitro and in sacco evaluation of total mixed ration silage added different levels of chitosanOriginal Paper

Ayu Septi Anggraeni ORCID...1, Anuraga Jayanegara ORCID...2, Ahmad Sofyan ORCID...3, Erika Budiarti Laconi ORCID...2, Nur Rochmah Kumalasari ORCID...2, I Nyoman Gunadarma ORCID...4, Hendra Herdian ORCID...3, Rusli Fidriyanto ORCID...5
1 Research Center for Food Technology and Processing, National Research and Innovation Agency, Gunungkidul, Indonesia
2 Graduate School of Nutrition and Feed Science, Faculty of Animal Science, IPB University (Bogor Agricultural University), Bogor, Indonesia
3 Research Center for Animal Husbandry – National Research and Innovation Agency, Bogor, Indonesia
4 Graduate School of Faculty of Animal Science Bogor Agricultural University (IPB University), Dramaga-Bogor, Indonesia
5 Research Center for Research Center for Applied Zoology – National Research and Innovation Agency, Bogor, Indonesia

This experiment aimed to evaluate the effects of chitosan administration to total mixed ration (TMR) silage on ruminal fermentation characteristics and degradability by using both in vitro and in sacco methods. The completely randomized design (CRD) used in this study included five treatments and six replications. The treatments consisted of TMR silage with distilled water as control (SA), TMR with 1% acetic acid (SB), 0.5% chitosan in 1% acetic acid diluent (SC), 1% chitosan in 1% acetic acid diluent (SD), and 1.5% chitosan in 1% acetic acid diluent (SE). The variables observed were ruminal fermentation parameters, methane production and in sacco nutrient degradability. Results revealed that adding 1–1.5% chitosan (SD and SE treatments) reduced (P < 0.05) total protozoa, total gas production, methane production, the percentage of acetic acid, and the ratio of acetic acid to propionic acid, while it increased (P < 0.05) the proportion of propionic acid. The addition of chitosan (SE treatment) decreased (P < 0.05) the degradation of dry matter, but it increased (P < 0.05) the degradability of organic matter and crude protein, as determined by in sacco analysis.

Keywords: chitosan; in vitro; in sacco; rumen modifier; silage

Received: December 16, 2023; Accepted: May 13, 2024; Prepublished online: May 30, 2024; Published: May 31, 2024  Show citation

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Anggraeni AS, Jayanegara A, Sofyan A, Laconi EB, Kumalasari NR, Gunadarma IN, et al.. In vitro and in sacco evaluation of total mixed ration silage added different levels of chitosan. Czech J. Anim. Sci. 2024;69(5):178-190. doi: 10.17221/173/2023-CJAS.
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