Czech J. Anim. Sci., 2023, 68(4):178-187 | DOI: 10.17221/132/2022-CJAS

Studies on the growth, immunomodulation and gut morphometry of Labeo rohita fed pectinOriginal Paper

Humaira Sehrish Hashmi1, Noor Khan1, Khalid Javed Iqbal2, Mahroze Fatima1, Khalid Mahmood Anjum3, Sumaira Abbas1, Muhammad Awais1, Sadia Nazir1, Muhammad Asghar1, Mahanama De Zoysa4
1 Department of Fisheries and Aquaculture, University of Veterinary and Animal Sciences, Lahore, Pakistan
2 Department of Zoology, The Islamia University of Bahawalpur, Bahawalpur, Pakistan
3 Department of Wildlife & Ecology, University of Veterinary and Animal Sciences, Lahore, Pakistan
4 College of Veterinary Medicine, Chungnam National University, Republic of Korea

The present study was designed to evaluate the effect of pectin supplemented feeds on the growth, gut morphology and immunology of Labeo rohita. Experimental feeds containing various concentrations of pectin including control (0%), T1 (0.5%), T2 (1.0%), T3 (2.0%), and T4 (4.0%) were administered to L. rohita twice per day at a rate of 3% body weight. There was a significant (P < 0.05) decrease in average final weight, weight gain%, net weight gain and specific growth rate observed in treatment groups in order of control > T1 > T2 > T3 > T4 while feed conversion ratio was significantly (P < 0.05) higher in T4 compared to the other groups T3 > T2 > T1 > control. Immunological parameters of peroxidase, protease and antiprotease (trypsin) showed significant (P < 0.05) results between control and treated groups. Peroxidase activity was significantly (P < 0.05) higher in T4 group fish compared to T3 > T2 > T1 > control group. Total protein, protease and antiprotease activity were significantly (P < 0.05) higher in the control compared to treatment groups T1 > T2 > T3 > T4. An increased level of the non-starch polysaccharide pectin in feed caused acute effects on gut morphometry. Villus length, villus width, and an increase in mucosal folds were abnormal but no significant (P > 0.05) abnormality was observed in the control group. In the submucosal part of the intestine, hyperplasia was shown in loose connective tissues and in adipose tissue cells in the treated groups. In conclusion, dietary pectin supplementation from 0.5% to 4% had negative effects on the growth, immunological parameters and gut histomorphology of L. rohita.

Keywords: rahu; weight gain; serum; plasma protein; peroxidase

Received: August 15, 2022; Accepted: February 1, 2023; Prepublished online: April 5, 2023; Published: April 24, 2023  Show citation

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Hashmi HS, Khan N, Iqbal KJ, Fatima M, Anjum KM, Abbas S, et al.. Studies on the growth, immunomodulation and gut morphometry of Labeo rohita fed pectin. Czech J. Anim. Sci. 2023;68(4):178-187. doi: 10.17221/132/2022-CJAS.
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