Czech J. Anim. Sci., 2020, 65(6):193-204 | DOI: 10.17221/23/2020-CJAS

Comparison of two muscle fibre staining techniques and their relation to pork quality traitsOriginal Paper

Nicole Lebedová ORCID...*,1, Tersia Needham2, Jaroslav Čítek1, Monika Okrouhlá1, Kateřina Zadinová1, Kamila Pokorná1, Roman Stupka1
1 Department of Animal Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic
2 Department of Animal Science and Food Processing, Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, Prague, Czech Republic

This study compared two histochemical staining methods of muscle fibres and evaluated their relationship with the meat quality traits of two high-value porcine muscles. Immunohistochemical (IHC) and adenosine triphosphatase (ATPase) staining was used to assess the cross-sectional area and proportion of fibre-types I, IIa, IIx and IIb in the samples of longissimus lumborum (LL) and psoas major (PM) muscles collected one-hour post-mortem from 25 crossbred pigs [Large WhiteSire × (Landrace × Large WhiteDam)] at an average age of 152 days. Muscles differed in all fibre parameters, except the proportion and relative area of type IIx fibres. The LL muscle exhibited greater fibre cross-sectional areas of all fibre types, higher proportions of type IIb/IIB, and lower proportions of I and IIa fibres than the PM muscle in both staining techniques. These two muscles also differed marginally in moisture, crude protein and intramuscular fat content. The PM muscle showed a low correlation between fibre types and chemical composition, but the LL muscle showed moderate correlations between fibre CSA and area composition for moisture and ash content. After IHC staining, an increase in LL eye muscle area and drip loss were correlated with lower proportions of type I fibres, while a greater proportion of type IIx fibres resulted in increased LL eye muscle area and moisture content. Furthermore, a higher CSA of all fibre types in the LL decreased redness (a*) and moisture content of the muscle. Results showed that IHC is more appropriate than ATPase staining for the assessment of relationships between muscle fibre parameters and meat quality traits in pigs.

Keywords: ATPase activity; histology; immunohistochemistry; muscle fibre type; myosin heavy chain isoform

Published: June 30, 2020  Show citation

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Lebedová N, Needham T, Čítek J, Okrouhlá M, Zadinová K, Pokorná K, Stupka R. Comparison of two muscle fibre staining techniques and their relation to pork quality traits. Czech J. Anim. Sci. 2020;65(6):193-204. doi: 10.17221/23/2020-CJAS.
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