Czech J. Anim. Sci., 2019, 64(11):439-447 | DOI: 10.17221/101/2018-CJAS

Expression analysis of BMPR1B, BMP15, and GDF9 in prolific and non-prolific sheep breeds during the follicular phaseOriginal Paper

Ji-Shun Tang1,2, Wen-Ping Hu1, Ran Di1, Xiang-Yu Wang1, Xiao-Sheng Zhang3, Jin-Long Zhang3, Qiu-Yue Liu*,1, Ming-Xing Chu*,1
1 Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P.R. China
2 Institute of Animal Husbandry and Veterinary Medicine, Anhui Academy of Agricultural Sciences, Hefei, P.R. China
3 Tianjin Institute of Animal Sciences, Tianjin, P.R. China

To elucidate how expression characteristics of BMPR1B, BMP15, and GDF9 are associated with sheep reproduction, tissue-specific mRNA expression of these genes in Small Tail Han (STH) ewes (a polytocous breed) and Sunite (SNT) ewes (a monotocous breed) in the follicular phase were investigated using transcription profiling and quantitative real-time PCR (qPCR). Expression levels of the three genes were all highest in ovaries of the two sheep breeds, and BMPR1B and GDF9 expression in the ovarian tissue was significantly higher in STH sheep compared with SNT sheep (P < 0.01), whereas BMP15 expression in pituitary, ovarian, oviduct, and uterine tissues was significantly lower in STH sheep compared with SNT sheep (P < 0.01). This study revealed that BMPR1B, BMP15, and GDF9 may play important roles in the hypothalamic-pituitary-gonadal axis, especially in the ovary, which may affect sheep prolificacy. BMPR1B and GDF9 expression might increase litter size, whereas BMP15 expression may decrease litter size. These results provide preliminary information regarding molecular regulatory mechanisms underlying sheep prolificacy.

Keywords: tissue; candidate gene; transcription profiling; qPCR; prolificacy

Published: November 30, 2019  Show citation

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Tang J, Hu W, Di R, Wang X, Zhang X, Zhang J, et al.. Expression analysis of BMPR1B, BMP15, and GDF9 in prolific and non-prolific sheep breeds during the follicular phase. Czech J. Anim. Sci. 2019;64(11):439-447. doi: 10.17221/101/2018-CJAS.
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