Czech J. Anim. Sci., 2018, 63(10):419-427 | DOI: 10.17221/121/2017-CJAS

Effect of supplementation with two combinations of alternative to antimicrobials by stages on cecal fermentation in rabbitsOriginal Paper

Lihui Liu, Yujing Xu, Xiurong Xu*
College of Animal Science and Technology, Northwest A&F University, Yangling, P.R. China

Antimicrobials inhibit cecal fermentation when preventing rabbit from infection. This study aimed to evaluate the effect of supplementation with two combinations of alternative to antimicrobial (combination I: 1 × 109 cfu/kg Bacillus subtilis + 2 g/kg fructooligosaccharide; combination II: 2 g/kg acidifier and 0.6 g/kg essential oil) by stages on rabbit's growth performance and cecal fermentation. Two hundred and forty 15-day-old male kits with similar body weight were distributed randomly to five groups, which were control (basal diet), ZnB (addition of 0.1 g/kg bacitracin zinc in basal diet), II (addition of combination II), I-II (addition of combination I during days 15-35, addition of combination II during days 36-77), and I-II-I (supplemented with combination I during days 15-35 and 57-77, supplemented with combination II during days 36-56). Each group had 6 replicates. One healthy rabbit from each replicate was slaughtered at day 35 and day 77. The results showed: (1) at day 35, the two combinations and bacitracin zinc all inhibited ileal Escherichia coli (P < 0.05), decreased cecal pH, and increased total volatile fatty acid concentration (P < 0.05). Combination І decreased duodenal crypt depth and increased duodenal villi height to crypt depth ratio (VCR) (P < 0.05); (2) at day 77, I-II-I group had more cecal total bacteria than control (P < 0.05). Mode I-II or I-II-I increased cecal Bacteroides-Prevotella (P < 0.05) compared with ZnB. Mode I-II-I shortened duodenal crypt depth and increased VCR compared with control or ZnB (P < 0.05); (3) after weaning, modes I-II-I and I-II had better or similar effect on decreasing diarrhoea and mortality rate compared with ZnB. In conclusion, both modes had better or similar effect on decreasing diarrhoea and mortality rate compared with inclusion of antimicrobial or combination II alone during the whole trial, and mode I-II-I showed better effect than mode I-II.

Keywords: Bacillus subtilis; acidifier; essential oil; fructooligosaccharide; fermentation trait

Published: October 31, 2018  Show citation

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Liu L, Xu Y, Xu X. Effect of supplementation with two combinations of alternative to antimicrobials by stages on cecal fermentation in rabbits. Czech J. Anim. Sci. 2018;63(10):419-427. doi: 10.17221/121/2017-CJAS.
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    References

    1. Cardinali R., Rebollar P.G., Dal Bosco A., Cagiola M., Moscati L., Forti K., Castellini C. (2008): Effect of dietary supplementation of organic acids and essential oils on immune function and intestinal characteristics of experimentally infected rabbits. In: Proc. 9th World Rabbit Congress, Verona, Italy, 573-578.
    2. Chaney A.L., Marbach E.P. (1962): Modified reagents for determination of urea and ammonia. Clinical Chemistry, 8, 130-132. Go to original source...
    3. Converse R.R., Blackwood A.D., Kirs M., Griffith J.F., Noble R.T. (2009): Rapid QPCR-based assay for fecal Bacteroides spp. as a tool for assessing fecal contamination in recreational waters. Water Research, 43, 4828-4837. Go to original source... Go to PubMed...
    4. de Blas C., Mateos G.G. (2010): Feed formulation. In: de Blas C. and Wiseman J. (eds): The Nutrition of the Rabbit. CABI Publishing, Wallingford, UK, 222-232. Go to original source...
    5. Denman S.E., McSweeney C.S. (2006): Development of a real-time PCR assay for monitoring anaerobic fungal and cellulolytic bacterial populations within the rumen. FEMS Microbiology Ecology, 58, 572-582. Go to original source... Go to PubMed...
    6. Euler A.R., Mitchell D.K., Kline R., Pickering L.K. (2005): Prebiotic effect of fructo-oligosaccharide supplemented term infant formula at two concentrations compared with unsupplemented formula and human milk. Journal of Pediatric Gastroenterology and Nutrition, 40, 157-164. Go to original source... Go to PubMed...
    7. Falcao-e-Cunha L., Marounek M., Freire J., Castro-Solla L., Maertens L., Pinheiro V. (2010): Alternatives to antibiotic growth promoters in rabbit feeding: a review. World Rabbit Science, 15, 127-140. Go to original source...
    8. Fekete S. (1989): Recent findings and future perspectives of digestive physiology in rabbits: A review. ActaVeterinaria Hungarica, 37, 265-279.
    9. Fortunlamothe L., Boullier S. (2007): A review on the interactions between gut microflora and digestive mucosal immunity. Possible ways to improve the health of rabbits. Livestock Science, 107, 1-18. Go to original source...
    10. Gopi M., Karthik K., Manjunathachar H.V., Tamilmahan P., Kesavan M., Dashprakash M., Purushothaman M.R. (2014): Essential oils as a feed additive in poultry nutrition. Advances in Animal and Veterinary Sciences, 2, 1-7. Go to original source...
    11. Guedes C.M., Mourcdo J.L., Silva S.R., Gomes M.J., Rodrigues M.A.M., Pinheiro V. (2009): Effects of age and mannanoligosaccharides supplementation on production of volatile fatty acids in the caecum of rabbits. Animal Feed Science and Technology, 150, 330-336. Go to original source...
    12. Hanson N.B., Lanning D.K. (2008): Microbial induction of B and T cell areas in rabbit appendix. Development and Comparative Immunology, 32, 980-991. Go to original source... Go to PubMed...
    13. Harrison A.P., Hansen P.A. (1963): Bacteroides hypermegas nov. spec. Antonie Van Leeuwenhoek, 29, 22-28. Go to original source... Go to PubMed...
    14. Hashemi S.R., Zulkifli I., Davoodi H., Zunita Z., Ebrahimi M. (2012): Growth performance, intestinal microflora, plasma fatty acid profile in broiler chickens fed herbal plant (Euphorbia hirta) and mix of acidifiers. Animal Feed Science and Technology, 178, 167-174. Go to original source...
    15. Huijsdens X.W., Linskens R.K., Mak M., Meuwissen S.G., Vandenbroucke-Grauls C.M., Savelkoul P.H. (2002): Quantification of bacteria adherent to gastrointestinal mucosa by real-time PCR. Journal of Clinical Microbiology, 40, 4423-4427. Go to original source... Go to PubMed...
    16. Iji P., Saki A.A., Tivey D.R. (2001): Intestinal structure and function of broiler chickens on diets supplemented with a mannan oligosaccharide. Journal of the Science of Food and Agriculture, 81, 1186-1192. Go to original source...
    17. Jacquier V., Combes S., Oswald I.P., Rogelgaillard C., Gidenne T. (2014): Early modulation of the cecal microbial activity in the young rabbit with rapidly fermentable fiber: impact on health and growth. Journal of Animal Science, 92, 5551-5559. Go to original source... Go to PubMed...
    18. Jenkins J.R. (2000): Rabbit and ferret liver and gastrointestinal testing. In: Fudge A.M. (ed.): Laboratory Medicine: Avian and Exotic Pets. WB Saunders, Philadelphia, USA, 291-304.
    19. Kapiki A., Costalos C., Oikonomidou C., Triantaf yllidou A., Loukatou E., Pertrohilou V. (2007): The effect of a fructo-oligosaccharide supplemented formula on gut flora of preterm infants. Early Human Development, 83, 335-339. Go to original source... Go to PubMed...
    20. Maenner K., Vahjen W., Simon O. (2011): Studies on the effects of essential-oil-based feed additives on performance, ileal nutrient digestibility, and selected bacterial groups in the gastrointestinal tract of piglets. Journal of Animal Science, 89, 2106-2112. Go to original source... Go to PubMed...
    21. Mao B., Li D., Zhao J., Liu X., Gu Z., Chen Y.Q. (2015): In vitro fermentation of fructooligosaccharides with human gut bacteria. Food and Function, 6, 947-954. Go to original source... Go to PubMed...
    22. Marty J., Vernay M. (1984): Absorption and metabolism of the volatile fatty acids in the hind-gut of the rabbit. British Journal of Nutrition, 51, 265-277. Go to original source... Go to PubMed...
    23. Mourao J.L., Pinheiro V., Alves A., Guedes C.M., Pinto L., Saavedra M.J., Kocher A. (2006): Effect of mannan oligosaccharides on the performance, intestinal morphology and cecal fermentation of fattening rabbits. Animal Feed Science and Technology, 126, 107-120. Go to original source...
    24. NRC (1977): Nutrient Requirements of Rabbits. 2 nd Ed. The National Academies Press, Washington, D.C., USA.
    25. Oso A.O., Idowu O.M.O., Haastrup A.S., Ajibade A.J., Olowonefa K.O., Aluko A.O., Bamgbose A.M. (2013): Growth performance, apparent nutrient digestibility, caecal fermentation, ileal morphology and caecal microflora of growing rabbits fed diets containing probiotics and prebiotics. Livestock Science, 157, 184-190. Go to original source...
    26. Pinheiro V., Guedes C.M., Outor-Monteiro D., Mourao J.L. (2009): Effects of fibre level and dietary mannanoligosaccharides on digestibility, caecal volatile fatty acids and performances of growing rabbits. Animal Feed Science and Technology, 148, 288-300. Go to original source...
    27. Pinheiro V., Mourao J.L., Alves A., Rodrigues M., Saavedra M.J. (2005): Effects of zinc bacitracin on performance, digestibility and caecal development of growing rabbits. In: Proc. 8th World Rabbit Congress, Pueblo, Mexico, 942-947.
    28. Rhee K.J., Sethupathi P., Driks A., Lanning D.K., Knight K.L. (2004): Role of commensal bacteria in development of gut-associated lymphoid tissues and preimmune antibody repertoire. Journal of Immunology, 172, 1118-1124. Go to original source... Go to PubMed...
    29. Romero C., Rebollar P.G., Moscati L., Bosco A.D., Castellini C., Cardinali R. (2012): Effect of substitution of medium-chain organic acids for zinc bacitracin in a diet containing colistin on performance and development of intestinal lymphoid tissues in growing rabbits experimentally infected with Escherichia coli O103 and Clostridium perfringens toxinotype A. Animal Feed Science and Technology, 174, 174-181. Go to original source...
    30. Si W., Gong J., Tsao R., Zhou T., Yu H., Poppe C., Du Z. (2006): Antimicrobial activity of essential oils and structurally related synthetic food additives towards selected pathogenic and beneficial gut bacteria. Journal of Applied Microbiology, 100, 296-305. Go to original source... Go to PubMed...
    31. Zhao X.H., Zhang T., Xu M., Yao J.H. (2011): Effects of physically effective fiber on chewing activity, ruminal fermentation, and digestibility in goats. Journal of Animal Science, 89, 501-509. Go to original source... Go to PubMed...
    32. Zhu K.H., Xu X.R., Sun D.F., Tang J.L., Zhang Y.K. (2014): Effects of drinking water acidification by organic acidifier on growth performance, digestive enzyme activity and caecal bacteria in growing rabbits. Animal Feed Science and Technology, 190, 87-94. Go to original source...

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