Czech J. Anim. Sci., 2018, 63(10):399-407 | DOI: 10.17221/117/2018-CJAS

Effect of thawing method on bull sperm survival in ejaculates frozen in 4 ml and 8 ml volumesOriginal Paper

Zuzana Biniová*,1,2, Luděk Stádník1, Martina Doležalová1, Jaromír Ducháček1
1 Department of Animal Husbandry, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic
2 NATURAL spol. s r.o., Hradištko pod Medníkem, Czech Republic

The aim of this study was to evaluate the effect of different thawing protocols (slow (P1), medium (P2), and fast (P3)) on percentage of motile sperm (MOT) and percentage of sperm cells with intact membranes (INT) in Holstein (4 bulls; 72 samples) and Czech Fleckvieh (4 bulls; 72 samples) semen frozen-thawed in 4 ml and 8 ml volumes. MOT was analysed in fresh semen, as well as immediately after thawing (T0) and 30 min after thawing (T30). INT was analysed using hypoosmotic swelling test (HOS test) in fresh ejaculate (FE), after diluting (DE), and at T0. The differences between FE parameters and frozen-thawed ejaculate parameters, expressing changes that occur during cryopreservation, were calculated. Apart from the effect of thawing protocol, the effect of breed and the effect of quality of FE expressed by MOT immediately after collecting were evaluated, too. Unlike thawing of semen cryopreserved in straws (0.25 and 0.5 ml), thawing using the slow protocol (P1) was the most appropriate (P < 0.05) for both observed volumes. There were found significantly higher MOT in the volume of 8 ml in both T0 and T30 and in the volume of 4 ml in T30 in samples thawed using P1 and P2. MOT in T0 was significantly affected by breed in samples frozen in 8 ml and in T30 in samples frozen in 4 and 8 ml. There were found no significant differences in INT in all reported volumes, however decrease of INT during cryopreservation was affected by breed.

Keywords: bovine semen; large volumes; semen processing, sperm quality parameters

Published: October 31, 2018  Show citation

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Biniová Z, Stádník L, Doležalová M, Ducháček J. Effect of thawing method on bull sperm survival in ejaculates frozen in 4 ml and 8 ml volumes. Czech J. Anim. Sci. 2018;63(10):399-407. doi: 10.17221/117/2018-CJAS.
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    References

    1. Arav A., Yavin S., Zeron Y., Natan D., Dekel I., Gacitua H. (2002): New trends in gamete's cryopreservation. Molecular and Cellular Endocrinology, 187, 77-81. Go to original source... Go to PubMed...
    2. Barth A.D., Bowman B., Bowman P.A. (1988): Determination of the best practical method of thawing bovine semen. Canadian Veterinary Journal, 29, 366-369.
    3. Beran J., Stadnik L., Duchacek J., Tousova R., Louda F., Stolc L. (2011): Effect of bulls' breed, age and body condition score on quantitative and qualitative traits of their semen. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 59, 37-44. Go to original source...
    4. Beran J., Stadnik L., Bezdicek J., Louda F., Citek J., Duchacek J. (2012): Effect of sire and extender on sperm motility and share of live or dead sperm in bulls' fresh ejaculate and in AI doses after thawing. Archiv für Tierzucht, 55, 207-218. Go to original source...
    5. Brannigan R., Lipshultz L. (2008): Sperm transport and capacitation. The Global Library of Women's Medicine. Available at http://editorial.glowm.com/?p=glowm.cml/section_view&articleid=315 (accessed May 13, 2017).
    6. Brinsko S.P., Van Wagner G.S., Graham J.K., Squires E.L. (2000): Motility, morphology and triple stain analysis of fresh, cooled and frozen-thawed stallion spermatozoa. Journal of Reproduction and Fertility, 56, 111-120.
    7. Dolezalova M., Stadnik L., Biniova Z., Duchacek J., Stupka R. (2016): Equilibration and freezing interactions affecting bull sperm characteristics after thawing. Czech Journal of Animal Science, 61, 515-525. Go to original source...
    8. Gaciuta H., Arav A. (2005): Successful pregnancies with directional freezing of large volume buck semen. Theriogenology, 63, 931-938. Go to original source... Go to PubMed...
    9. Gloria A., Carluccio A., Contri A., Wegher L., Valorz C., Robbe D. (2013): The effect of chamber on kinetic results in cryopreserved bull spermatozoa. Andrology, 1, 879-885. Go to original source... Go to PubMed...
    10. Holt W.V., Del Valle I., Fazeli A. (2015): Heat shock protein A8 stabilizes the bull sperm plasma membrane during cryopreservation: Effects of breed, protein concentration, and mode of use. Theriogenology, 84, 693-701. Go to original source... Go to PubMed...
    11. Horvath A., Miskolczi E., Mihalffy S., Osz K., Szabo K., Urbanyi B. (2007): Cryopreservation of common carp (Cyprinus carpio) sperm in 1.2 and 5 ml straws and occurrence of haploids among larvae produced with cryopreserved sperm. Cryobiology, 54, 251-257. Go to original source... Go to PubMed...
    12. James P.S., Wolfe C.A., Mackie A., Ladha S., Prentice A., Jones R. (1999): Lipid dynamics in the plasma membrane of fresh and cryopreserved human spermatozoa. Human Reproduction, 14, 1827-1832. Go to original source... Go to PubMed...
    13. Jeyendran R.S., Van der Ven H.H., Perez-Pelaez M., Crabo B.G., Zaneveld L.J.D. (1984): Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. Journal of Reproduction and Fertility, 70, 219‒225. Go to original source... Go to PubMed...
    14. Kohsaka T., Takahara H., Tagami S., Sasada H., Masaki J. (1992): A new technique for the precise location of lactate and malate dehydrogenases in goat, boar and water buffalo spermatozoa using gel incubation film. Journal of Reproduction and Fertility, 95, 201-209. Go to original source... Go to PubMed...
    15. Lepcock J.R., Frey H.E., Ritchie K.P. (1993): Protein denaturation in intact hepatocytes and isolated cellular organelles during heat shock. Journal of Cell Biology, 122, 1267-1276. Go to original source... Go to PubMed...
    16. Li Y., Sun M., Zhu J., Jiao G., Lin J., Dong H., Tan J., Zhou J. (2017): Species difference in the expression of Fas and Fas ligand in mature mammalian spermatozoa. Czech Journal of Animal Science, 62, 519-526. Go to original source...
    17. Maxwell W.M.C., Parrilla I., Caballero I., Garcia E., Roca J., Martinez E.A., Vazquez J.M., Rath D. (2007): Retained functional integrity of bull spermatozoa after double freezing and thawing using PureSperm® density gradient centrifugation. Reproduction in Domestic Animals, 42, 489-494. Go to original source... Go to PubMed...
    18. Mortimer S.T. (2000): CASA - practical aspects. Journal of Andrology, 21, 515-524. Go to original source... Go to PubMed...
    19. Padrik P., Hallap T., Kaart T., Bulitko T., Jaakma U. (2012): Relationships between the results of hypo-osmotic swelling tests, sperm motility, and fertility in Estonian Holstein dairy bulls. Czech Journal of Animal Science, 57, 490-497. Go to original source...
    20. Pons-Rejraji H., Bailey J.L., Leclerc P. (2009): Cryopreservation affects bovine sperm intracellular parameters associated with capacitation and acrosome exocytosis. Reproduction, Fertility and Development, 21, 525-537. Go to original source... Go to PubMed...
    21. Ramu S., Jeyendran R.S. (2013): Thy hypo-osmotic swelling test for evaluation of sperm membrane integrity. Methods of Molecular Biology, 927, 21-25. Go to original source... Go to PubMed...
    22. Rastegarnia A., Shahverdi A., Rezaei T.T., Ebrahimi B., Shafipour V. (2013): Effect of different thawing rates on post-thaw viability, kinematic parameters and chromatin structure of buffalo (Bubalus bubalis) spermatozoa. Cell Journal, 14, 306-313.
    23. Saragusty J., Gaciuta H., Pettit M.T. (2007): Directional freezing of equine semen in large volumes. Reproduction in Domestic Animals, 42, 610-615. Go to original source... Go to PubMed...
    24. Saragusty J., Gacitua H., Zeron Y., Rozenboim I., Arav A. (2009): Double freezing of bovine semen. Animal Reproduction Science, 115, 10-17. Go to original source... Go to PubMed...
    25. Si W., Hildebrandt T.B., Reid C., Krieg R., Ji W., Fassbender M., Hermes R. (2006): The successful double cryopreservation of rabbit (Oryctolagus cuniculus) semen in large volume using the directional freezing technique with reduced concentration of cryoprotectant. Theriogenology, 65, 788-798. Go to original source... Go to PubMed...
    26. Sichtar J., Nehasilova A., Simonik O., Bubenickova F. (2017): Effect of two freezing extenders on characteristic of fresh and frozen-thawed semen in endangered Old Kladruber stallions - a pilot study. Czech Journal of Animal Science, 62, 227-233. Go to original source...
    27. Simonik O., Sichtar J., Krejcarkova A., Rajmon R., Stadnik L., Beran J., Dolezalova M., Biniova Z. (2015): Computer Assisted Sperm Analysis - the relationship to bull field fertility, possible errors and their impact on outputs: A review. Indian Journal of Animal Sciences, 85, 3-11. Go to original source...
    28. Slanina T., Miskeje M., Tirpak F., Blaszczyk M. (2018): Effect of taurine on turkey (Meleagris gallopavo) spermatozoa viability and motility. Czech Journal of Animal Science, 63, 127-135. Go to original source...
    29. Stadnik L., Rajmon R., Beran J., Simonik O., Dolezalova M., Sichtar J., Stupka R., Folkova P. (2015): Influence of selected factors on bovine spermatozoa cold shock resistance. Acta Veterinaria Brno, 84, 125-131. Go to original source...
    30. Tasdemir U., Buyukleblebici S., Tuncer P.B., Coskun E., Ozgurtas T., Aydin A.N., Buyukleblebici O., Gurcan I.S. (2013): Effects of various cryoprotectants on bull sperm quality, DNA integrity and oxidative stress parameters. Cryobiology, 66, 38-42. Go to original source... Go to PubMed...
    31. Thurston L.M., Watson P.F., Holt W.V. (2002): Semen cryopreservation: A genetic explanation for species and individual variation? Cryo Letters, 23, 255-262.
    32. Tvrda E., Greifova H., Mackovich A., Hashim F., Lukac N. (2018): Curcumin offers antioxidant protection to cryopreserved bovine semen. Czech Journal of Animal Science, 63, 247-255. Go to original source...
    33. Underwood S.L., Bathgate R., Maxwell W.M.C., Evans G. (2009): In vitro characteristics of frozen-thawed, sex sorted bull sperm after refreezing or incubation at 15 or 37°C. Theriogenology, 72, 1001-1008. Go to original source... Go to PubMed...
    34. Underwood S.L., Bathgate R., Maxwell W.M.C., Evans G. (2010): Birth of offspring after artificial insemination of heifers with frozen-thawed, sex-sorted, re-frozen-thawed bull sperm. Animal Reproduction Science, 118, 171-175. Go to original source... Go to PubMed...
    35. Verstegen J., Iguer-Ouada M., Onclin K. (2002): Computer assisted semen analyzers in andrology research and veterinary practice. Theriogenology, 57, 149-179. Go to original source... Go to PubMed...
    36. Watson P.F. (2000): The causes of reduced fertility with cryopreserved semen. Animal Reproduction Science, 60, 481-492. Go to original source... Go to PubMed...

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