Czech J. Anim. Sci., 2018, 63(7):256-262 | DOI: 10.17221/96/2017-CJAS

L-selectin gene polymorphism and its association with clinical mastitis, somatic cell score, and milk production in Polish Holstein-Friesian cattleOriginal Paper

Magdalena Dusza*,1, Joanna Pokorska1, Joanna Makulska1, Dominika Kulaj1, Michal Cupial2
1 Department of Cattle Breeding, Institute of Animal Sciences, University of Agriculture in Krakow, Krakow, Poland
2 Institute of Agricultural Engineering and Informatics, University of Agriculture in Krakow, Krakow, Poland

Bovine mastitis is a widespread disease of the mammary gland, highly contributing to the increase in veterinary costs in dairy industry. In the present study, the genetic polymorphism within bovine L-selectin gene was analysed and its impact on clinical mastitis occurrence, somatic cell score (SCS), and milk production traits in Polish Holstein-Friesian cows was examined. Polymorphism within L-selectin gene, molecule responsible for neutrophil attachment to endothelium, might have a potential role in immune response to bacterial infections and udder health. Two hundred and six Polish Holstein-Friesian cows were genotyped by polymerase chain reaction-restriction fragment length polymorphism method. Two single nucleotide polymorphisms mutations within the coding sequence of L-selectin gene were identified (c.165G>A and c.567C>T). The effect of c.165G>A and c.567C>T mutations on SCS was highly significant (P = 0.0019 and P = 0.0003, respectively). Strong associations (P ≤ 0.0001) were also observed between L-selectin polymorphism and milk production traits (milk yield, milk fat percentage, and milk protein percentage). However, the polymorphism in the analysed gene had no influence on the resistance or susceptibility of cows to clinical mastitis (only the tendency toward significance, P = 0.06 for c.567C>T mutation was found). Potential exploitation of the information on the identified associations in genetic selection needs to confirm the obtained results in further investigations.

Keywords: SELL; udder; inflammation; milk production traits; cows

Published: July 31, 2018  Show citation

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Dusza M, Pokorska J, Makulska J, Kulaj D, Cupial M. L-selectin gene polymorphism and its association with clinical mastitis, somatic cell score, and milk production in Polish Holstein-Friesian cattle. Czech J. Anim. Sci. 2018;63(7):256-262. doi: 10.17221/96/2017-CJAS.
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    References

    1. Alhussien M., Manjari P., Sheikh A.A., Mohammed Seman S., Reddi S., Mohanty A.K., Mukherjee J., Dang A.K. (2016): Immunological attributes of blood and milk neutrophils isolated from crossbred cows during different physiological conditions. Czech Journal of Animal Science, 61, 223-231. Go to original source...
    2. Barrett J.C., Fry B., Maller J., Daly M.J. (2005): Haploview: analysis and visualization of LD and haplotype maps. Bioinformatics, 21, 263-265. Go to original source... Go to PubMed...
    3. Diez-Fraile A., Meyer E., Duchateau L., Burvenich C. (2003): L-selectin and β2-integrin expression on circulating bovine polymorphonuclear leukocytes during endotoxin mastitis. Journal of Dairy Science, 86, 2334-2342. Go to original source... Go to PubMed...
    4. Diez-Fraille A., Mehrzad J., Meyer E., Duchateau L., Burvenich C. (2004): Comparison of L-selectin and Mac-1 expression on blood and milk neutrophils during experimental Escherichia coli-induced mastitis in cows. American Journal of Veterinary Research, 65, 1164-1171. Go to original source... Go to PubMed...
    5. Edwards S.M., Thomsen B., Madsen P., Sorensen P. (2015): Partitioning of genomic variance reveals biological pathways associated with udder health and milk production traits in dairy cattle. Genetics Selection Evolution, 47: 60. Go to original source... Go to PubMed...
    6. Futosi K., Fodor S., Mocsai A. (2013): Neutrophil cell surface receptors and their intracellular signal transduction pathways. International Immunopharmacology, 17, 638-650. Go to original source... Go to PubMed...
    7. Heringstad B., Klemetsdal G., Ruane J. (2000): Selection for mastitis resistance in dairy cattle: a review with focus on the situation in the Nordic countries. Livestock Production Science, 64, 95-106. Go to original source...
    8. Heringstad B., Rekaya R., Gianola D., Klemetsdal G., Welgel K.A. (2003): Genetic change for clinical mastitis in Norwegian cattle: a threshold model analysis. Journal of Dairy Science, 86, 369-375. Go to original source... Go to PubMed...
    9. Hill A.W., Shears A.L., Hibbit K.G. (1979): The pathogenesis of experimental Escherichia coli mastitis in newly calved dairy cows. Journal of Dairy Science, 26, 97-101. Go to original source...
    10. Iso-Touru T., Sahana G., Guldbrandtsen B., Lund M.S., Vilkki J. (2016): Genome-wide association analysis of milk yield traits in Nordic Red Cattle using imputed whole genome sequence variants. BMC Genetics, 22, 17-55. Go to original source... Go to PubMed...
    11. Karthikeyan A., Radhika G., Aravindhakshan T.V., Anilkumar K. (2016): Expression profiling of innate immune genes in milk somatic cells during subclinical mastitis in crossbred dairy cows. Animal Biotechnology, 27, 303-309. Go to original source... Go to PubMed...
    12. Kishimoto T.K., Rothlein R. (1994): Integrins, ICAMs, and selectins: role and regulation of adhesion molecules in neutrophil recruitment to inflammatory sites. Advances in Pharmacology, 25, 117-169. Go to original source... Go to PubMed...
    13. Leyva-Baca I., Schenkel F., Sharma B.S., Jansen G.B., Karrow N.A. (2007): Identification of single nucleotide polymorphisms in the bovine CCL2, IL8, CCR2 and IL8RA genes and their association with health and production in Canadian Holsteins. Animal Genetics, 38, 198-202. Go to original source... Go to PubMed...
    14. Mallard B.A., Emam M., Paibomesai M., Thompson-Crispi K., Wagter-Lesperance L. (2015): Genetic selection of cattle for improved immunity and health. Japanese Journal of Veterinary Research, 63, S37-S44.
    15. Negussie E., Stranden I., Mantysaari E.A. (2008): Genetic analysis of liability to clinical mastitis, with somatic cell score and production traits using bivariate threshold-linear and linear-linear models. Livestock Science, 117, 52-59. Go to original source...
    16. Olsen H.G., Knutsen T.M, Lewandowska-Sabat A.M., Grove H., Nome T., Svendsen M., Arnyasi M., Sodeland M., Sundsaasen K.K., Dahl S.R., Heringstad B., Hansen H.H., Olsaker I., Kent M.P., Lien S. (2016): Fine mapping of a QTL on bovine chromosome 6 using imputed full sequence data suggests a key role for the groupspecific component (GC) gene in clinical mastitis and milk production. Genetics Selection Evolution, 48: 79. Go to original source... Go to PubMed...
    17. Oltenacu P.A., Broom D.M. (2010): The impact of genetic selection for increased milk yield on the welfare of dairy cows. Animal Welfare, 19, 39-49. Go to original source...
    18. Paape M.J., Bannerman D.D., Zhao X., Lee J.W. (2003): The bovine neutrophil: structure and function in blood and milk. Veterinary Research, 34, 597-627. Go to original source... Go to PubMed...
    19. Pant S., Schenkel F., Leyva-Baca I., Sharma B., Karrow N. (2007): Identification of single nucleotide polymorphisms in bovine CARD15 and their associations with health and production traits in Canadian Holsteins. BMC Genomics, 8: 421. Go to original source... Go to PubMed...
    20. Petrovski K.R., Trajcev M., Buneski G. (2006): A review of the factors affecting the costs of bovine mastitis. Journal of the South African Veterinary Association, 77, 52-60. Go to original source... Go to PubMed...
    21. Pokorska J., Kulaj D., Dusza M., Zychlinska-Buczek J., Makulska J. (2016): New rapid method of DNA isolation from milk somatic cells. Animal Biotechnology, 27, 113-117. Go to original source... Go to PubMed...
    22. Prin-Mathieu C., Le Roux Y., Faure G.C., Laurent F., Bene M.C., Moussaoui F. (2002): Enzymatic activities of bovine peripheral blood leukocytes and milk polymorphonuclear neutrophils during intramammary inflammation caused by lipopolysaccharide. Clinical and Diagnostic Laboratory Immunology, 9, 812-817. Go to original source... Go to PubMed...
    23. Purcell S., Neale B., Todd-Brown K., Thomas L., Ferreira M.A., Bender D., Maller J., Sklar P., de Bakker P.I., Daly M.J., Sham P.C. (2007): PLINK: a tool set for whole-genome association and population-based linkage analyses. American Journal of Human Genetics, 81, 559-575. Go to original source... Go to PubMed...
    24. Rupp R., Boichard D. (1999): Genetic parameters for clinical mastitis, somatic cell score, production, udder type traits, and milking ease in first lactation Holsteins. Journal of Dairy Science, 82, 2198-2204. Go to original source... Go to PubMed...
    25. Schennink A., Stoop W.M., Visker M.H., van der Poel J.J., Bovenhuis H., van Arendonk J.A. (2009): Short communication: Genome-wide scan for bovine milk-fat composition. II. Quantitative trait loci for long-chain fatty acids. Journal of Dairy Science, 92, 4676-4682. Go to original source... Go to PubMed...
    26. Swain D.K. (2016): Surface expression of CD11b, CD62L, CD44 receptors on blood and milk neutrophils during subclinical and clinical mastitis in Sahiwal cows. Indian Journal of Animal Sciences, 86, 250-255. Go to original source...
    27. Thompson-Crispi K., Atalla H., Miglior F., Mallard B.A. (2014): Bovine mastitis: frontiers in immunogenetics. Frontiers in Immunology, 5: 493. Go to original source... Go to PubMed...
    28. Van Wer ven T., Noordhuizen-Stassen E.N., Daemen A.J.J.M., Shukken Y.H., Brand A., Burvenich C. (1997): Preinfection in vitro chemotaxis, oxidative burst, and expression of CD11/CD18 receptors and their predictive capacity on the outcome of mastitis induced in dairy cows with Escherichia coli. Journal of Dairy Science, 80, 67-74. Go to original source... Go to PubMed...
    29. Weber P.S., Toelboell T., Chang L.C., Tirrell J.D., Saama P.M., Smith G.W., Burton J.L. (2004): Mechanisms of glucocorticoid-induced down-regulation of neutrophil L-selectin in cattle: evidence for effects at the geneexpression level and primarily on blood neutrophils. Journal of Leukocyte Biology, 75, 815-827. Go to original source... Go to PubMed...

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